Question

A facility with 100 L fermentation broth production capacity, Glucose will be used as substrate for biotransformation. Taking into account the microorganism production capacity and maintenance, determine the yield, productivity, annual operating cost and investment cost calculations related to production,
Also, determine the Monod kinetic constants

Answer 1

The fermentation broth is a very complex soup or solution. Fundamentally, the fermentation broth is the sea of nutrients in which the microorganisms grow, reproduce and 'swim' . ... The fermentation broth too act as the medium for various physical, biochemical and physical reactions to take place.

The Monod equation is a mathematical model for the growth of microorganisms.

The empirical Monod equation is:

{\displaystyle \mu =\mu _{\max }{S \over K_{s}+S}}

where:

• μ is the specific growth rate of the microorganisms
• μmax is the maximum specific growth rate of the microorganisms
• S is the concentration of the limiting substrate for growth
• Ks is the "half-velocity constant"—the value of S when μ/μmax = 0.5

μmax and Ks are empirical coefficients to the Monod equation. They will differ between microorganism species and will also depend on the ambient environmental conditions