Question

immunology

Q1:An immunologist stained macrophages by immunofluorescence using an antibody for the TAP1/TAP2 complex. Which intracellular compartments would exhibit positive staining with this antibody? And why?

*Immunofluorescence is a technique to visualize proteins or antigens inside cells by using fluorescent dyes linked to antibodies. We can see colors under the immunofluorescence microscope.

Q2:An immunologist wants to conduct 2 experiments; activate T cells and stop T cells activation. Advise her which cytokines she should choose. And why?

Answer 1

IMMUNOFLUORESCENCE:

TAP1 and TAP2 proteins belong to the ABC superfamily. Both of them act as a transportar protein. The main function is to transport intracellular short peptide to the MHC-I and them this peptide is displayed to the outer surface of the cell for display to the cytotoxic T-cell. For this transportation, the TAP1 and TAP2 protein make a heterodimeric complex  in the menbrane of the Endoplasmic reticulum and then deliver the short peptide that they are holding into the leumen of the ER. So, when a immunofluorescence is done that target TAP1 and TAP2, will definitely stain the ER.

ACTIVATION AND DEACTIVATION OF T-CELL:

If an immunologist want to conduct an experiment that include activation and deactivation of T-cell then the immunologist will choose IL-2, IL-10 and  IL-4. Here the different ILs(interleukins) are different types of cytokines which either activate or proliferate or deactivate the activity of the different T-cells. IL-2 activate T-cell and IL-4 and IL-10 deactivate activity and further proliferation of T-cells.