To-Air-is-Human, Inc. (TAH) manufactures lightweight unpowered and human-powered aircraft. They have decided to produce a brand-new low-cost two-seater glider kit for the consumer market, which they will sell direct to the consumer.  They will have a dedicated facility that they rent for $100,000 for the year. They will also rent two large molding machines for fabrication of the glider hull at $4500 apiece. The production line will be manned by four skilled workers, whose salaries total $115,000 for the year. Hull parts will total 680 lbs. of high-grade aluminum, which may be purchased at a delivered price of $2300/ton. Other parts will be contracted for inclusion in the kit: four windows per plane at $95 per window, two padded seats (with built-in seat assembly) at $110 apiece, steering mechanism at $225, all ailerons and louvers in a set for $400, and fixed landing gear for $175. The crate for shipping the product costs $40 for a single kit, and all shipping costs are assumed by the consumer. Advertising in a glider enthusiast magazine costs $500 for the year.  The advertised price will be $6999. How many glider kits does TAH have to sell in order to break even?

Follow up question: Neiman-Marcus wants to sell the TAH glider exclusively through their upscale Christmas catalog, and is willing to buy 120 of them from TAH, if TAH will chip in $16,000 for the catalog distribution. If all other parameters (except Q , of course, and the fact that they would no longer advertise in the magazine) from part A are the same, what breakeven price should TAH charge Neiman Marcus?

Explain how human resource management and human resource information systems evolve over time. Explain which HRIS types your current or previous employer utilizes. If your current or previous organization does not utilize a HRIS, which types would you recommend? How does the utilization of those systems promote transformational HR activities?

Why is feedback from HRIS customers/users important to a HRIS implementation team? Explain your experiences with HRIS as both an employee and non-employee. Next, explain how N-tier architecture or cloud computing has simplified HRIS usage and maintenance?

The inside of a cell is extremely crowded. We will try to gain some insight for exactly how crowded by considering some numbers that are estimates of the situation in the common bacterium E. coli. One can estimate the total number of proteins present in a cell in several ways. For example, one can measure the dry mass of a cell (the amount of material left once the cell is dried out), analyze the composition to determine what fraction is amino acids, estimate the average molecular weight of a protein, and use all these numbers to calculate the total number of protein molecules present. Alternatively, one can use experimental analysis to determine the approximate amount of proteins present in a sample consisting of a known number of cells. All of these methods agree that a typical E. coli bacterial cell contains about 3 x 10⁶ proteins.

a) We can estimate the volume of an E. coli cell to be about 1 µm³, that is, equivalent to a cube of 1 µm on each side. Convert that volume to liters, and assuming there are 3 x 10⁶ total proteins present, calculate the total molar concentration of proteins contained in a bacterial cell.

b) Take the reciprocal of the molar concentration obtained in part a, and convert units to find the number of cubic nanometers per protein molecule.

c) One way to think about the volume per protein molecule found in part b is that if you divided up the total volume of the cell into tiny cubes, with each cube having the volume found in part b, then on averge, each cube would contain one protein. The length of each side of one of these cubes would just be the cube root of the volume per protein molecule. Further, the length of each side of the cube is an estimate of the center-to-center separation distance of each protein molecule. From the volume per protein obtained in part b, use this approach to estimate the center-to-center distance between protein molecules (on average) in an E. coli cell.

d) Averaged across a cell's inventory of proteins, the average protein molecular weight is about 30000 grams per mole (i.e., 30 kiloDaltons). For proteins, an average mass density is found of about 1.4 g/cm³. Use these numbers, after appropriate unit conversions, to calculate the average volume of a protein molecule in cubic nanometers. Compare this number to the value obtained in part b, and estimate what fraction of the total volume of a cell is filled with proteins.

1. A superinfection will most likely occur from taking and antibiotic that

A. targets translation.

B. is of the beta-lactam class.

C. is static.

2. Which of the following is a mechanism by which acquired resistance can occur?

A. mutation of an antibiotic target

B. using a pump to remove antibiotic from the cytoplasm

C. producing an enzyme that metabolizes the antibiotic

D. all of the answers are correct

3. Antimicrobials that are effective against a wide variety of microbial types are termed

A. antibiotics

B. narrow-spectrum drugs

C. semi-synthetic drugs

D. synthetic drugs

E. broad-spectrum drugs

Which of the following would be an example of an alteration in drug effect because of a pharmacodynamic change?

Question 5 options:

Arthur is conducting an experiment of plasmid insertion. He has prepared some live bacteria and gone through all essential steps of plasmid insertion in a correct manner:

i) Cut open the plasmid by restriction enzyme and insert the gene by DNA ligase.

ii) Insert the plasmid into bacteria by heat shock.

iii) Grow the plasmid-containing bacteria in culture plate. However, after incubation, he failed to observe any successful cloning.

Assuming the enzymes involved could function properly.

List and expain FIVE possible errors that Arthur may have and lead to this unexpected result.

1. An increase in which of the following serum components would induce an episode of gout?

2. Which of these intermediates in cholesterol biosynthesis is the substrate for the enzyme at the key regulatory step of the pathway?

3. Which of the following is NOT involved in the activation of glucose for glycogen synthesis?

Which of the following statements about DNA repair is correct

Group of answer choices

during excision repair, an exonuclease is the first enzyme involved in removing a single-stranded segment of DNA from the template strand

none of the answer is correct

during mismatch repair, a methylated guanine in the consensus sequence GATC found on the parent strand activates the Mut complex

the Okazaki fragments synthesized during base excision repair are synthesized in a 3’ -- > 5’ fashion

repair of methylated DNA requires a set of proteins operating with the following order: endonuclease, exonuclease, DNA polymerase I, ligase and demethylase

Using circles to depict glucoses, draw a glycogen molecule with a main branch containing 12 glucose monomers. From the 5th glucose starting from the reducing end, draw a branch with 6 glucose monomers (Total 18 glucose monomers).

b)) Consider a liver cell with a debranching enzyme that lacks the ability hydrolyze 1,6 glycosidic linkages (it still has its other activity; all other enzymes are also working correctly). If this cell digests the above glycogen molecule, what would the products of this digestion look like? Draw the structure using circles to depict glucoses.

A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood enzyme reactions. It has been studied extensively and we know the two critical side chains in the active site of TIM that catalyze the isomerization reaction. Write the mechanism of TIM showing the two catalytic side chains.

B) In the TIM reaction, the histidine residue starts off neutral, not positively charged (as one might expect since it donates a proton to the O-group). Explain why it makes energetic sense for the histidine not to start off positively charged.