1. Describe any five Employee Sourcing Challenges facing Human Resource Managers in Organizations Today

1. A superinfection will most likely occur from taking and antibiotic that

A. targets translation.

B. is of the beta-lactam class.

C. is static.

2. Which of the following is a mechanism by which acquired resistance can occur?

A. mutation of an antibiotic target

B. using a pump to remove antibiotic from the cytoplasm

C. producing an enzyme that metabolizes the antibiotic

D. all of the answers are correct

3. Antimicrobials that are effective against a wide variety of microbial types are termed

A. antibiotics

B. narrow-spectrum drugs

C. semi-synthetic drugs

D. synthetic drugs

E. broad-spectrum drugs

Which of the following would be an example of an alteration in drug effect because of a pharmacodynamic change?

Question 5 options:

Arthur is conducting an experiment of plasmid insertion. He has prepared some live bacteria and gone through all essential steps of plasmid insertion in a correct manner:

i) Cut open the plasmid by restriction enzyme and insert the gene by DNA ligase.

ii) Insert the plasmid into bacteria by heat shock.

iii) Grow the plasmid-containing bacteria in culture plate. However, after incubation, he failed to observe any successful cloning.

Assuming the enzymes involved could function properly.

List and expain FIVE possible errors that Arthur may have and lead to this unexpected result.

1. An increase in which of the following serum components would induce an episode of gout?

2. Which of these intermediates in cholesterol biosynthesis is the substrate for the enzyme at the key regulatory step of the pathway?

3. Which of the following is NOT involved in the activation of glucose for glycogen synthesis?

Which of the following statements about DNA repair is correct

Group of answer choices

during excision repair, an exonuclease is the first enzyme involved in removing a single-stranded segment of DNA from the template strand

none of the answer is correct

during mismatch repair, a methylated guanine in the consensus sequence GATC found on the parent strand activates the Mut complex

the Okazaki fragments synthesized during base excision repair are synthesized in a 3’ -- > 5’ fashion

repair of methylated DNA requires a set of proteins operating with the following order: endonuclease, exonuclease, DNA polymerase I, ligase and demethylase

Using circles to depict glucoses, draw a glycogen molecule with a main branch containing 12 glucose monomers. From the 5th glucose starting from the reducing end, draw a branch with 6 glucose monomers (Total 18 glucose monomers).

b)) Consider a liver cell with a debranching enzyme that lacks the ability hydrolyze 1,6 glycosidic linkages (it still has its other activity; all other enzymes are also working correctly). If this cell digests the above glycogen molecule, what would the products of this digestion look like? Draw the structure using circles to depict glucoses.

A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood enzyme reactions. It has been studied extensively and we know the two critical side chains in the active site of TIM that catalyze the isomerization reaction. Write the mechanism of TIM showing the two catalytic side chains.

B) In the TIM reaction, the histidine residue starts off neutral, not positively charged (as one might expect since it donates a proton to the O-group). Explain why it makes energetic sense for the histidine not to start off positively charged.

1. If the pO2 is 30 mmHg in a given tissue, is more or less O bound at pH 7.6 compared with that bound at pH 7.2. Explain.

2. What role does CO2 play in determining/regulating blood pH? Explain.

3. Bis-phosphoglycerate (BPG) binds to hemoglobin. Discuss where and why it binds and the effect it has on the oxygen affinity of hemoglobin.

4. Describe two similarities and two differences between myoblobin and hemoglobin.

5. When the enzyme trypsin is denatured and then renatured it only regains 8% of its activity. Why is full activity not regained as it is with RNAase?

Consider the following kinetic data for an enzyme. Construct both an M&M and Lineweaver-Burke plots using this data. Estimate the Km and Vmax from both graphs and compare the values. Discuss any differences you see between the numbers you derive from the two different plots.