Questions
What does it mean for a lake to be stratified? What kind of tests can be...

What does it mean for a lake to be stratified? What kind of tests can be done to show whether a lake is or is not stratified? Please explain in detail.

In: Biology

SLO2- Understand how factors in the social structure, such as the economy, public policy, religion, and...

SLO2- Understand how factors in the social structure, such as the economy, public policy, religion, and education influence patterns and changes in marriage, families, and relationships.

For this question students will choose and discuss TWO factors in the social structure and their influence. The basics for this are touched upon in chapter one but every chapter from CH3 – CH14 discusses various factors in the social structure and the influence they have so students will need to choose, be specific, and focus on the detail for one example of influence/change per factor.

Choose a factor in the social structure, define it in your own words, then explain using an example, a specific way that this factor has influenced patterns and/or changes in either marriage, families, or relationships. Detail and terminology are expected.

Choose a different factor in the social structure, define it in your own words, then explain using an example, a specific way that this factor has influenced patterns and/or changes in either marriage, families, or relationships. Detail and terminology are expected.

In: Psychology

Escherichia coli Pseudomonas malli Bacillus megaterium Bacillusanthracis, Lactobacillusacidophilus, Pseudomonas aeruginosa, Serratia marcescens Staphylococcus caprae Streptococcus mutans...

Escherichia coli

Pseudomonas malli

Bacillus megaterium

Bacillusanthracis,

Lactobacillusacidophilus,

Pseudomonas aeruginosa,

Serratia marcescens

Staphylococcus caprae

Streptococcus mutans

Neisseria gonorrhoeae,

Staphylococcus aureus,

Streptococcus pyogenes,

Proteus myxofaciens,

Klebsiella pneumoniae sub sp pneumoniae

Salmonella bongrori

Shigella sonnei

Corynebacterium xerosis

Enterobacter aerogenes

Proteus vulgaris

Escherichia vulneris

Escherichia blattae

Salmonella choleraesuis (subsp. diarizonae)

Enterobacter hormaechei

Enterobacter asburiae

Citrobacter diversus

Citrobacter freudii

In order to receive full credit for this assignment, you must provide the following;

The correct identification of Unknown organism in this homework assignment.

The correct interpretation of the biochemical tests in the table below for this unknown organism.

Create a flow chart which demonstrates the tests that allowed you separate the unknown organism from the list.

Use Microsoft PowerPoint, it is easier for creating flow charts compared to Microsoft Word

You do not need to isolate the entire list of organisms from each other, just your unknown from the list. See the sample flow chart.

Using your biochemical and morphological results to indentify the identity of your orphan organism by using the Bergey’s manual.

Create a flow chart and identify your orphan organism.

UNKNOWN # 3

The follow biochemical tests were performed after an unknown bacterium was isolated into a pure culture. Each of the tests was incubated at 37°C for 24 hours and when appropriate the use of reagents was added in the correct volumes to each of the tests. The following table describes these results.

      

      

Name of the Biochemical Test/ Media

Appearance after addition of the reagent

POS/NEG

Interpret the Resulting Reactions of the test

( POS or NEG) 1 pts each

Catalase (example)

Bubbles

POS

POS for catalase/ H2O2  àH20 + O2

Urea/ Urea broth

Reddish / yellow

Phenol red Lactose

Red

Phenol red Mannitol

Yellow

Simmons Citrate

Blue

Methyl red (MR)/ MRVP broth

Red

Vogus Proskauer (VP)/MRVP broth

No change/copper color

Indole production

/ SIM media

No red ring

Phenol red Glucose

Yellow

Gram Stain

Pink

FTM tube/ fluid thioglycollate media

Growth though out the tube

0.4% Agar with Nutrient broth and1% TTC.

Growth and Red though out the tube

Starch Hydrolysis/ Starch agar

Brown around the inoculation

Gelatin Hydrolysis/ Gelatin media

Solid

Oxidase

No color change

Nitrate reduction/ Nitrate broth

Red After Nitrate A&B reagent is added

Shape

ROD

Fat hydrolysis/ Spirit blue agar

White around the inoculation

H2S production/ SIM media

Black

In: Biology

Q1) Explain the difference between contractionary and expansionary fiscal policy. Regarding the business cycle, when would...

Q1) Explain the difference between contractionary and expansionary fiscal policy. Regarding the business cycle, when would you use each type of fiscal policy? Explain you answer with sufficient detail so the instructor has clear evidence that you under the difference between the policies, the types of action each policy might entail, and the economic impact of each.

In: Economics

4- In detail using the variables measured in this lab (pulmonary function, respiratory rate, and heart...

4- In detail using the variables measured in this lab (pulmonary function, respiratory rate, and heart rate), explain potential physiological benefits an elite level athlete might gain by exercising at high altitude and then competing at sea level compared to someone who only trained at sea level; and explain why these changes are only temporary. Use at least 5 references

In: Mechanical Engineering

Executive Summary The executive summary is a company marketing document intended to excite potential investors, customers,...

Executive Summary

The executive summary is a company marketing document intended to excite potential investors, customers, advisers, and employees. Its purpose is to get the call back and start the serious discussions—the process that will result in action.

Your executive summary should include:

  • The Mission Statement
  • Company information
  • Growth highlights - Graphs and charts can be helpful in this section.
  • Your Products/Services
  • Financial information – If you are seeking financing, include any information about your current bank and investors.
  • Summarize future plans –
  • The elevator speech—for this assignment it will take the place of an abstract. It should be crisp, clear, and convey what the company is all about in less than 250 words.

This paper should have a title page, abstract (the elevator speech), body, and reference page. Points will be deducted if any of these four sections are missing. Make sure papers are written in correct APA style.

The executive summary should be between 400-600 words (not including the elevator speech/abstract), double space, and use font size 12. Structure the paper along the lines advised above.

In: Economics

JAVA Input a phrase from the keyboard, if the phrase contains "red" or "RED" print out...

JAVA Input a phrase from the keyboard, if the phrase contains "red" or "RED" print out "red" . if the phrase contains "blue" or "BLUE" output "blue" In all other cases print "No Color" For example: If the input was "Violets are BLUE" your output should be "blue" If the input was "Singing the blues" output "blue" If the input was "I have a pure bred puppy" your output should be "red" If the input was "Today is Monday" output "No color". If the input was "My shirt is Blue" output "No color". (because you are only looking for "blue", "BLUE", "red", "RED")

In: Computer Science

Answer the following about metabolic syndrome in as much detail as you can recall (10 pts)...

Answer the following about metabolic syndrome in as much detail as you can recall (10 pts)
         i. What is metabolic syndrome and what are the causes or the conditions that cause and/or associated with metabolic syndrome? (3 pts)
             (type answer here)

         ii. What are the metabolic/biochemical characteristics of metabolic syndrome? (remember this is a biochemistry class and pathway and signaling and specific reaction discussions should be included with this answer – what pathways are affected and what is happening to them? ) (4 pts)
            (type answer here)

          iii. What factors have been shown to help alleviate the effects of metabolic syndrome and their effects on metabolism? Explain why these factors or changes have had these effects on metabolic syndrome

In: Biology

The age of distribution of the Canadian population and the age distribution of a random sample...

The age of distribution of the Canadian population and the age distribution of a random sample of 455 residents in the Indian community of Red Lake Village (Northwest Territories) are shown in the chart. Use a 5% level of significance to test the claim that the age distribution of the general Canadian population fits the age distribution of the residents of Red Lake Village.

Age (years)

Percent of Canadian Population

Observed Number in Red Lake Village

under 5

7.2%

47

5 to 14

13.6%

75

15 to 64

67.1%

288

65 and older

12.1%

45

What can you conclude?

In: Statistics and Probability

Questions 1 You are the principle investigator of a research unit in the Botany and Plant...

Questions 1

You are the principle investigator of a research unit in the Botany and Plant Biotechnology department that studies medicinal plant usage in South Africa. Your work focused extensively on devil’s claw. You have identified that one of the plant extracts, harpagoside that is an iridoid glycoside, is of medicinal use. You would like to produce this iridoid glycoside in a liquid culture of Escherichia coli at commercial scale. You have identified that the key enzyme that you would need to introduce into E. coli is an iridoid synthase from devil’s claw. This gene is known and studied in Arabidopsis thaliana but remains to be identified from devil’s claw. How would you go about using the known Arabidopsis sequence to isolate the full length gene from devil’s claw? Describe the steps that you will follow in detail and provide all the details of the techniques that you use in each step and how your approach will impact on these steps. Hint: It is critical to remember that devil’s claw is a eukaryote and E. coli, where you want to express the iridoid synthase protein, is a prokaryote.                                                                                              (29)

Question 2

How will you ensure that the PCR amplicon that you have obtained is the correct gene from devil’s claw? Describe the steps that you will follow in detail and provide all the details of the techniques that you use in each step.                                                                                                                                                              (11)

Question 3

You want to clone and express the gene that you’ve isolated from devil’s claw in E. coli. Describe all the necessary adjustments that you would need to make to your gene to ensure correct expression in E. coli.                                                                                                                                                                                 (5)

Question 4

Describe all the steps that you would take to clone your gene into E. coli. Draw a diagram of your vector containing the gene insert. Discuss how you will identify a clone in an E. coli cell that you can work further with. Describe the steps that you will follow in detail and provide details of the techniques that you use in each step and how your approach will impact on these steps.                          (25)

In: Biology