Using 1.0 nM enzyme X for the enzyme kinetics experiments, you obtained the reaction rates at different concentrations of substrate (shown in the following table). Assume that the enzyme X follows a typical Michaelis-Menten kinetics.
[substrate] (μM) Reaction rate (μmol/s)
2 7
5 17
10 29
20 44
50 67
100 80
200 89
500 95
1000 98
a) Estimate the Vmax and Km values of the enzyme X in this reaction using the double reciprocal plot.
b) If 5.0 nM of the enzyme X is used in the experiment, what will be the Vmax and Km values of the enzyme X?
In: Chemistry
elemental analysis of an unknown compound was found to give the following data 70.4% carbon, 8.08% hydrogen and 10.12 % of nitrogen. it was found to have a molecular weight of 137.18 Da. determine the empirical formula, molecular formula, and the degree of unsaturation for this compound
In: Chemistry
Evaluate the integral by making an appropriate change of variables.
3 cos
|
|||||||
| R |
where R is the trapezoidal region with vertices (6, 0), (10, 0), (0, 10), and (0, 6)
In: Math
In: Operations Management
Which of the following would be expected to lower the Tm for a phospholipid bilayer?
replacing a lipid containing unsaturated fatty acids with one containing saturated fatty acids.
replacing a lipid containing 18-C fatty acids with one containing 16-C fatty acids.
adding ions to bind to charged groups in the polar head groups.
decreasing the concentration of phospholipids.
In: Chemistry
In: Biology
On its income statement for a recent year, Continental Airlines, Inc. reported a net loss of $68 million from operations. On its statement of cash flows, it reported $457 million of cash flows from operating activities.
Explain this apparent contradiction between the loss and the positive cash flows.
In: Accounting
Use the genetic code and the table that indicates the base
pairing that occurs according to the wobble hypothesis to answer
the following.
What is the three letter abbreviation of your favorite amino acid?
How many codons specify this amino acid?
What is the minimum number of tRNAs required to recognize the codons that specify this amino acid?
What is/are the anticodon sequence(s) of this amino acid? Enter your responses in the 5' to 3' direction.
In: Biology
IN DETAIL EXPLAIN the Regulation of the glycolysis pathway in eukaryotic cells
In: Biology
The active site of an enzyme is a small portion of the enzyme molecule
What is the function of the rest of the huge molecule?
Check all that apply.
| To pass through cell membranes. |
| To provide sites for enzyme control and regulation. |
| To hold the substrate in place. |
| To stabilize the three-dimensional structure of the enzyme. |
| To align the active-site functional groups that catalyze the reaction. |
In: Other