Questions
1. Why does E. coli need both DNA polymerase III and DNA polymerase I? a. The...

1. Why does E. coli need both DNA polymerase III and DNA polymerase I?

a. The DNA replication is bidirectional; one polymerase is used for each direction.

b. Each polymerase is specific for only one strand of DNA. DNA polymerase III acts only on the leading strand, and DNA polymerase I acts only on the lagging strand.

c. Only DNA polymerase I has proofreading ability.

d. DNA polymerase III lacks the 5' → 3' exonuclease activity needed to remove RNA primers.

2. The replication of DNA is ________ because ________.

a. semiconservative; one strand of parental DNA is retained in each daughter DNA.

b. semiconservative; each daughter molecule has two new strands copied from the parental DNA template

c. conservative; each daughter molecule has two new strands copied from the parental DNA template.

d. conservative; one strand of parental DNA is retained in each daughter DNA.

3. Active transport proteins in biological membranes are

a. always transporting Ca2+ ions.

b. never driven by concentration gradients.

c. never driven by ATP to ADP conversion.

d. sometimes driven by concentration gradients.

4. Why should it not be surprising that for many cells water requires a protein for its transport across a membrane?

a. The transport protein is needed to prevent the hydrolysis of the phospholipid chains as water crosses the membrane.

b. Water is very polar which inhibits its free diffusion across the membrane.

c. All molecules require transport proteins to cross a membrane.

d. There is never a concentration gradient for water across the membrane to drive its transport.

5. The conversion of pyruvate to lactate in muscles also causes the ________.

a. reduction of NAD+.

b. oxidation of NAD+

c. oxidation of NADH

d. reduction of oxygen to water.

6. ATP is a cosubstrate of the enzyme PFK-1. In most species ATP is also an inhibitor of PFK-1 at higher concentrations. Which statement below would provide a suitable explanation?

a. PFK-1 must be phosphorylated by ATP in the active site and the phosphorylated PFK-1 must be the less active form.

b. There are two sites on PFK-1 that bind ATP. One is the active site; the other is the regulatory site where inhibition occurs allosterically.

c. ATP actually activates the reverse of the reaction preceding the PFK-1 step in the pathway. It likely has no direct effect on PFK-1.

d. There must be another cofactor interacting with ATP at high concentrations to achieve inhibition of PFK-1.

7. Briefly describe two ways for deactivation of GPCR-signaling.

In: Chemistry

Problem Statement: 1: Using a pivot table, show in an appropriate chart the total number of...

Problem Statement:

1: Using a pivot table, show in an appropriate chart the total number of confirmed voters by percentage and by age group in the United States

2: What conclusions do you draw from the study and chart?

State Age Total Population Citizen Population Registered Voters Confirmed Voters
Alabama 18 to 24 439000 428000 212000 155000
Alabama 25 to 34 576000 535000 359000 271000
Alabama 35 to 44 615000 582000 410000 330000
Alabama 45 to 64 1297000 1275000 1051000 939000
Alabama 65+ 667000 660000 523000 459000
Alaska 18 to 24 63000 61000 31000 20000
Alaska 25 to 34 109000 103000 70000 46000
Alaska 35 to 44 86000 80000 60000 50000
Alaska 45 to 64 186000 182000 144000 122000
Alaska 65+ 72000 69000 55000 51000
Arizona 18 to 24 586000 545000 301000 234000
Arizona 25 to 34 859000 709000 399000 289000
Arizona 35 to 44 870000 713000 437000 382000
Arizona 45 to 64 1656000 1502000 1015000 901000
Arizona 65+ 892000 846000 659000 606000
Arkansas 18 to 24 288000 281000 126000 70000
Arkansas 25 to 34 362000 336000 194000 140000
Arkansas 35 to 44 357000 341000 234000 205000
Arkansas 45 to 64 742000 704000 498000 442000
Arkansas 65+ 449000 448000 324000 267000
California 18 to 24 3962000 3479000 1787000 1447000
California 25 to 34 5357000 4153000 2548000 2070000
California 35 to 44 5030000 3675000 2478000 2118000
California 45 to 64 9356000 7827000 5459000 4926000
California 65+ 4653000 4284000 3084000 2902000
Colorado 18 to 24 493000 461000 278000 259000
Colorado 25 to 34 693000 602000 411000 363000
Colorado 35 to 44 684000 616000 484000 452000
Colorado 45 to 64 1354000 1287000 1025000 989000
Colorado 65+ 593000 577000 437000 432000
Connecticut 18 to 24 333000 308000 145000 123000
Connecticut 25 to 34 402000 331000 210000 177000
Connecticut 35 to 44 441000 382000 255000 223000
Connecticut 45 to 64 1047000 993000 768000 695000
Connecticut 65+ 503000 486000 383000 351000
Delaware 18 to 24 82000 77000 38000 33000
Delaware 25 to 34 106000 86000 54000 49000
Delaware 35 to 44 119000 102000 77000 67000
Delaware 45 to 64 240000 232000 182000 168000
Delaware 65+ 146000 145000 118000 113000
District Of Columbia 18 to 24 71000 61000 43000 38000
District Of Columbia 25 to 34 154000 133000 114000 102000
District Of Columbia 35 to 44 79000 67000 59000 55000
District Of Columbia 45 to 64 140000 129000 109000 99000
District Of Columbia 65+ 73000 71000 60000 57000
Florida 18 to 24 1669000 1467000 751000 614000
Florida 25 to 34 2259000 1903000 1158000 987000
Florida 35 to 44 2456000 1960000 1335000 1177000
Florida 45 to 64 5345000 4814000 3474000 3107000
Florida 65+ 3304000 3182000 2384000 2222000
Georgia 18 to 24 920000 867000 469000 349000
Georgia 25 to 34 1308000 1148000 847000 700000
Georgia 35 to 44 1341000 1228000 825000 726000
Georgia 45 to 64 2658000 2547000 1886000 1741000
Georgia 65+ 951000 948000 739000 652000
Hawaii 18 to 24 107000 91000 33000 24000
Hawaii 25 to 34 172000 154000 65000 55000
Hawaii 35 to 44 166000 153000 83000 69000
Hawaii 45 to 64 343000 317000 217000 196000
Hawaii 65+ 224000 215000 149000 137000
Idaho 18 to 24 130000 121000 51000 39000
Idaho 25 to 34 194000 173000 114000 99000
Idaho 35 to 44 203000 187000 141000 132000
Idaho 45 to 64 401000 382000 274000 250000
Idaho 65+ 201000 201000 164000 160000
Illinois 18 to 24 1228000 1122000 608000 396000
Illinois 25 to 34 1707000 1480000 1020000 846000
Illinois 35 to 44 1688000 1481000 1066000 872000
Illinois 45 to 64 3407000 3182000 2471000 2206000
Illinois 65+ 1620000 1566000 1259000 1108000
Indiana 18 to 24 570000 558000 269000 203000
Indiana 25 to 34 861000 800000 462000 356000
Indiana 35 to 44 828000 801000 550000 420000
Indiana 45 to 64 1754000 1733000 1329000 1212000
Indiana 65+ 840000 832000 660000 610000
Iowa 18 to 24 260000 243000 150000 121000
Iowa 25 to 34 402000 379000 276000 238000
Iowa 35 to 44 385000 358000 275000 238000
Iowa 45 to 64 828000 812000 652000 586000
Iowa 65+ 444000 440000 392000 365000
Kansas 18 to 24 294000 262000 127000 88000
Kansas 25 to 34 406000 359000 236000 172000
Kansas 35 to 44 300000 269000 203000 169000
Kansas 45 to 64 757000 721000 583000 535000
Kansas 65+ 363000 363000 318000 285000
Kentucky 18 to 24 446000 437000 225000 165000
Kentucky 25 to 34 513000 462000 320000 235000
Kentucky 35 to 44 547000 534000 411000 330000
Kentucky 45 to 64 1173000 1152000 879000 757000
Kentucky 65+ 613000 608000 468000 408000
Louisiana 18 to 24 431000 415000 226000 174000
Louisiana 25 to 34 579000 552000 422000 369000
Louisiana 35 to 44 543000 526000 399000 340000
Louisiana 45 to 64 1199000 1184000 968000 846000
Louisiana 65+ 568000 562000 483000 420000
Maine 18 to 24 118000 118000 61000 50000
Maine 25 to 34 134000 131000 93000 78000
Maine 35 to 44 157000 149000 117000 101000
Maine 45 to 64 413000 407000 329000 300000
Maine 65+ 219000 215000 187000 170000
Maryland 18 to 24 585000 558000 301000 246000
Maryland 25 to 34 777000 621000 439000 393000
Maryland 35 to 44 721000 590000 434000 396000
Maryland 45 to 64 1557000 1450000 1107000 1025000
Maryland 65+ 809000 787000 607000 550000
Massachusetts 18 to 24 694000 633000 395000 316000
Massachusetts 25 to 34 810000 705000 526000 450000
Massachusetts 35 to 44 888000 783000 580000 530000
Massachusetts 45 to 64 1784000 1677000 1424000 1327000
Massachusetts 65+ 994000 975000 833000 759000
Michigan 18 to 24 905000 894000 536000 394000
Michigan 25 to 34 1182000 1107000 830000 629000
Michigan 35 to 44 1221000 1136000 900000 768000
Michigan 45 to 64 2676000 2611000 2141000 1906000
Michigan 65+ 1512000 1480000 1214000 1134000
Minnesota 18 to 24 531000 510000 329000 303000
Minnesota 25 to 34 650000 596000 435000 384000
Minnesota 35 to 44 701000 659000 518000 478000
Minnesota 45 to 64 1410000 1387000 1151000 1081000
Minnesota 65+ 762000 751000 652000 613000
Mississippi 18 to 24 300000 294000 222000 187000
Mississippi 25 to 34 397000 390000 333000 279000
Mississippi 35 to 44 320000 309000 255000 222000
Mississippi 45 to 64 734000 727000 614000 563000
Mississippi 65+ 416000 410000 371000 337000
Missouri 18 to 24 525000 520000 332000 223000
Missouri 25 to 34 857000 809000 585000 448000
Missouri 35 to 44 669000 651000 498000 427000
Missouri 45 to 64 1714000 1684000 1344000 1175000
Missouri 65+ 755000 745000 624000 545000
Montana 18 to 24 82000 79000 53000 33000
Montana 25 to 34 138000 137000 77000 65000
Montana 35 to 44 109000 105000 69000 62000
Montana 45 to 64 254000 249000 201000 189000
Montana 65+ 186000 184000 153000 146000
Nebraska 18 to 24 168000 151000 71000 61000
Nebraska 25 to 34 242000 224000 137000 110000
Nebraska 35 to 44 235000 218000 145000 128000
Nebraska 45 to 64 462000 445000 331000 300000
Nebraska 65+ 264000 259000 217000 200000
Nevada 18 to 24 320000 295000 145000 122000
Nevada 25 to 34 364000 310000 189000 158000
Nevada 35 to 44 307000 247000 158000 146000
Nevada 45 to 64 680000 606000 413000 376000
Nevada 65+ 369000 350000 270000 246000
New Hampshire 18 to 24 123000 119000 71000 61000
New Hampshire 25 to 34 160000 147000 99000 91000
New Hampshire 35 to 44 150000 143000 100000 90000
New Hampshire 45 to 64 425000 414000 337000 312000
New Hampshire 65+ 171000 169000 145000 134000
New Jersey 18 to 24 792000 707000 379000 276000
New Jersey 25 to 34 1068000 842000 556000 475000
New Jersey 35 to 44 1186000 939000 655000 536000
New Jersey 45 to 64 2424000 2217000 1732000 1545000
New Jersey 65+ 1259000 1224000 1004000 838000
New Mexico 18 to 24 177000 163000 84000 66000
New Mexico 25 to 34 286000 248000 157000 130000
New Mexico 35 to 44 257000 224000 139000 123000
New Mexico 45 to 64 588000 551000 414000 390000
New Mexico 65+ 246000 240000 185000 169000
New York 18 to 24 2028000 1790000 926000 710000
New York 25 to 34 2775000 2171000 1387000 1145000
New York 35 to 44 2334000 1871000 1288000 1133000
New York 45 to 64 5217000 4706000 3381000 3046000
New York 65+ 2712000 2543000 1904000 1642000
North Carolina 18 to 24 863000 779000 496000 389000
North Carolina 25 to 34 1220000 1021000 816000 676000
North Carolina 35 to 44 1320000 1172000 919000 792000
North Carolina 45 to 64 2470000 2373000 1925000 1710000
North Carolina 65+ 1390000 1366000 1138000 1057000
North Dakota 18 to 24 67000 66000 43000 32000
North Dakota 25 to 34 111000 106000 74000 62000
North Dakota 35 to 44 71000 69000 55000 44000
North Dakota 45 to 64 194000 190000 148000 129000
North Dakota 65+ 85000 84000 64000 61000
Ohio 18 to 24 1145000 1105000 629000 511000
Ohio 25 to 34 1413000 1352000 917000 772000
Ohio 35 to 44 1340000 1274000 903000 772000
Ohio 45 to 64 3213000 3179000 2325000 2159000
Ohio 65+ 1640000 1640000 1302000 1181000
Oklahoma 18 to 24 325000 323000 130000 88000
Oklahoma 25 to 34 567000 540000 296000 184000
Oklahoma 35 to 44 433000 418000 281000 212000
Oklahoma 45 to 64 1001000 980000 720000 602000
Oklahoma 65+ 483000 473000 378000 345000
Oregon 18 to 24 391000 364000 216000 186000
Oregon 25 to 34 461000 399000 256000 215000
Oregon 35 to 44 527000 479000 345000 296000
Oregon 45 to 64 1004000 961000 756000 709000
Oregon 65+ 616000 604000 513000 491000
Pennsylvania 18 to 24 1198000 1137000 645000 478000
Pennsylvania 25 to 34 1543000 1442000 984000 790000
Pennsylvania 35 to 44 1520000 1442000 1054000 928000
Pennsylvania 45 to 64 3674000 3547000 2648000 2331000
Pennsylvania 65+ 1913000 1883000 1463000 1297000
Rhode Island 18 to 24 99000 89000 61000 45000
Rhode Island 25 to 34 121000 108000 70000 58000
Rhode Island 35 to 44 150000 127000 89000 76000
Rhode Island 45 to 64 290000 272000 213000 185000
Rhode Island 65+ 158000 155000 119000 105000
South Carolina 18 to 24 474000 451000 265000 243000
South Carolina 25 to 34 510000 476000 364000 293000
South Carolina 35 to 44 601000 561000 405000 343000
South Carolina 45 to 64 1319000 1289000 956000 866000
South Carolina 65+ 612000 604000 489000 442000
South Dakota 18 to 24 95000 94000 52000 31000
South Dakota 25 to 34 103000 101000 65000 46000
South Dakota 35 to 44 82000 79000 64000 53000
South Dakota 45 to 64 204000 202000 167000 145000
South Dakota 65+ 132000 130000 106000 96000
Tennessee 18 to 24 610000 590000 306000 207000
Tennessee 25 to 34 833000 773000 517000 395000
Tennessee 35 to 44 783000 739000 514000 402000
Tennessee 45 to 64 1607000 1563000 1081000 904000
Tennessee 65+ 1016000 1013000 793000 698000
Texas 18 to 24 2538000 2249000 971000 572000
Texas 25 to 34 3695000 2965000 1734000 1228000
Texas 35 to 44 3445000 2748000 1839000 1473000
Texas 45 to 64 6115000 5429000 3981000 3399000
Texas 65+ 2849000 2671000 2225000 1971000
Utah 18 to 24 279000 263000 115000 97000
Utah 25 to 34 446000 407000 236000 194000
Utah 35 to 44 357000 307000 182000 161000
Utah 45 to 64 592000 576000 396000 375000
Utah 65+ 242000 240000 208000 195000
Vermont 18 to 24 61000 59000 30000 23000
Vermont 25 to 34 69000 68000 41000 30000
Vermont 35 to 44 78000 76000 53000 46000
Vermont 45 to 64 195000 193000 153000 133000
Vermont 65+ 93000 91000 79000 75000
Virginia 18 to 24 695000 612000 351000 292000
Virginia 25 to 34 1192000 1026000 698000 605000
Virginia 35 to 44 989000 889000 717000 634000
Virginia 45 to 64 2140000 2050000 1591000 1473000
Virginia 65+ 1079000 1069000 853000 774000
Washington 18 to 24 563000 511000 275000 215000
Washington 25 to 34 1017000 911000 596000 488000
Washington 35 to 44 916000 809000 649000 577000
Washington 45 to 64 1660000 1570000 1183000 1101000
Washington 65+ 1074000 1032000 830000 791000
West Virginia 18 to 24 163000 162000 78000 37000
West Virginia 25 to 34 213000 210000 131000 66000
West Virginia 35 to 44 237000 237000 162000 111000
West Virginia 45 to 64 479000 475000 326000 253000
West Virginia 65+ 361000 359000 286000 222000
Wisconsin 18 to 24 539000 513000 308000 287000
Wisconsin 25 to 34 699000 662000 475000 438000
Wisconsin 35 to 44 714000 695000 548000 514000
Wisconsin 45 to 64 1552000 1532000 1224000 1192000
Wisconsin 65+ 847000 845000 763000 697000
Wyoming 18 to 24 56000 55000 21000 18000
Wyoming 25 to 34 73000 71000 44000 39000
Wyoming 35 to 44 68000 66000 41000 36000
Wyoming 45 to 64 155000 154000 101000 95000
Wyoming 65+ 74000 73000 61000 59000

In: Operations Management

Part I—Prenatal Visit It had been a hectic day for the doctor, and his last patient...

Part I—Prenatal Visit It had been a hectic day for the doctor, and his last patient was on time for her appointment. “Hi. Sis.” “Hi, Jim. How’s my kid brother?” “Tired—how’s the first-time mom? Have you stopped work yet?” “Come on, Jim, I’m a career woman. Te chemical industry needs me. It’s tough to go cold turkey. When Dave and I decided to have a family, we agreed that I should work as long as I could.” “You’re the size of a house, Jen. You’re 37 years old, you have twins that are 28 weeks, and I really would like you to carry them as long as you can.” “Oh, oh, here comes the lecture again. I know you didn’t like the fertility procedure we did, but I just couldn’t get pregnant. Anyway, as you so tactfully pointed out, doctor, I am not getting any younger. Look at it this way; we’re having our family all at once.” Tere was a knock at the door and the nurse entered. “How did the ultrasound go?” “It’s like Times Square,” replied the nurse. “Arms and legs everywhere—it’s almost impossible to see anything, and those kids are so active. I just hope they slow down after they’re born, otherwise you’ll get no rest until they go to college. It’s crazy in there, with two hearts beating. And with yours in the background, it’s tough to isolate one. As best as I can tell, the rates are around 130 to 160 beats a minute.” “OK, well let’s see if the expert can hear anything.” Both women glared at the doctor. “Do you need the crane or can you get up on the table by yourself?” Jen looked at the nurse and said, “Is he like to this to all his patients?” “No comment; I need this job.” Tey laughed and Jim went to work. Moving the head of his stethoscope to different locations, he tried to isolate the sounds from one heart. His expression changed as he listened intently. Jen began to worry as her brother took longer than usual with his stethoscope. “Is everything OK?” she asked. “Anyone Who Had A Heart” by Phil Stephens Page 1 Jim looked concerned. He was reluctant to tell his sister that he thought he had heard a heart murmur. Like his nurse had said, it was difficult to hear just one heart. He didn’t want to alarm his sister, so he made a joke of the situation. “Must be a girl in there, she won’t stop talking. I think she said something about not leaving the hospital in just any outfi t.” Jen went to hit her brother, but he easily evaded the playful smack. “It’s tough to predict when the babies will arrive, so I think you should start coming to see me every week, Jen.” “Is there a problem? You did all of the tests, and you said that I’m not diabetic. Te alpha-fetoprotein levels indicate no neural tube defects, and that horrible amniocentesis procedure showed no birth defects or Rhesus problems.” “It’s OK, Sis. It’s quite normal for women who are close to delivery to visit their OB-GYN every week. It’s tough to predict delivery with multiple births, because they rarely go full term. So, to be on the safe side, let’s get together every week from now on. In that way I can see your lovely smiling face more often.” Jen scowled at her brother. “For once in your life, do what I ask,” Jim said. “And bring Dave with you next time.” A little later, as Jim was finishing his paper work for the day, the nurse returned to his office. “Everything OK with Jen?” “I can’t stop thinking about that vague sound I heard. It sounded like a heart murmur, but it’s so tough to single out with two kids in there.” “I thought I heard it too. Te babies seem active and are about the same size. No history of birth heart defects in either family, but I guess you never know with twins in an older mom.” “My thoughts exactly. I don’t want to worry her because you know how hyper she can be. I guess we’ll need to keep a close eye on her, and on my nephew and niece.” “Does Jen know the sexes?” “She and Dave don’t want to know. Tey want to be surprised.” Questions 1. Draw a diagram of the heart of a human adult. Show the circulation of the blood through the heart and to and from the body and the lungs. 2. Draw a diagram of the heart of a human fetus. Show the circulation of the blood through the heart and to and from the body and the lungs. 3. What are the major structural differences between the fetal and the adult human heart? Draw and label these differences on your diagram of the fetal heart. How do these structures alter the circulation of blood? 4. Why is the pulmonary circulation reduced in the human fetus? 5. Are heart rates of 130 to 160 normal in a human fetus? “Anyone Who Had A Heart” by Phil Stephens Page 2 Part II—The Arrival Te ambulance that carried Jen sped through the crowded streets with its sirens blasting and lights fl ashing. T e traffic parted, and people watched as the vehicle made its way to the hospital. “My water had to break right in the middle of a meeting.” “Don’t worry, Jen. I’m sure they understand.” Her husband, Dave, was trying to comfort her, but then, true to form, he said something wrong. “Anyway, it’ll give them something to talk about around the coff ee machine.” Jen thought how lucky she was that her husband worked in the same office, but she didn’t like the idea of being the topic of office gossip. She was beginning to feel motion sickness from lying on a stretcher in a moving vehicle. Just then the ambulance stopped, its doors opened, and she was being wheeled down a long corridor. Finally, the stretcher turned a corner and she felt Dave leave her side as she entered a delivery room. She felt the sting of a needle in her left hand. Ten she felt Dave’s hand slip into hers and saw his eyes like slits between the cap and the surgical mask he had put on. Jen felt a needle go into her back and almost immediately the lower half of her body felt numb; the epidural had taken effect. She looked up into the mirror and saw the surgeon with a child in his hands. Te noise of first one crying baby and then another filled the room. Jen held the babies and everyone, except Dave and the nurse, left the room. Te babies were passed between the two of them as the nurse performed various tests. “I came as quickly as I could,” Jim said as he entered the room like a hurricane. Jen thought that he had been late for almost everything in his life, but she would forgive him this time. Jim kissed his sister, smiled at his brother-in-law, and then went over to the nurse. Jen and Dave could hear them talking softly. “No problem, doctor.” Jim came over to the new parents. “So, one of each. Any names picked out?” “Brianna and Christopher,” said Jen. “OK, Doc.” Dave smiled because he knew that Jim hated being called Doc. “Why were they blue when they were born?” “Tat’s quite normal, but they should have changed to pink a minute or so after they were born.” “So why does Brianna still have blue finger tips and lips?” asked Jen. “It’s called cyanosis; it’s not uncommon. Don’t worry. One advantage of being related to a doctor is that all three of you will get first-class treatment.” “I hope so, because Chris has pink lips, and he’s breathing at a much slower rate than his sister.”

Questions 1. What vital signs or symptoms do the two babies exhibit?

2 What is cyanosis?

3. Would you be alarmed that Brianna has cyanosis and Christopher does not?

Why? 4. Why would the cyanotic baby have a faster breathing rate?

5. What are the possible diagnoses for Brianna’s condition at this time?

For each diagnosis that you come up with, describe the signs or symptoms that relate to that diagnosis.

In: Biology

Pick the correcty answer If a mutation that inactivated telomerase occurred in a cell (telomerase activity...

Pick the correcty answer

If a mutation that inactivated telomerase occurred in a cell (telomerase activity in the cell = zero), what do you expect the outcome to be?

The position of the centromere would shift at each replication cycle, eventually leading to mutations in the genetic information and cell death.
The number of tandem repeats would increase with each replication cycle, eventually leading to a large unstable chromosome and cell death.
The telomeres would shorten at each replication cycle, eventually leading to loss of essential genetic information and cell death.
DNA primase would be unable to bind to the 5 end of the template strand, eventually causing a reduction in chromosome size and cell death.

Somatic or body cells have very little telomerase. As a result, the telomeres within these (proliferating) cells _____ with each cell division.

shorten
lengthen
remain constant in size
can either shorten or lengthen
None of the answers is correct.

A researcher is studying telomerase in four types of mouse cells: brain cells, skin cells, blood cells, and gametes (sperm/eggs). What do you suspect she will find?

telomerase activity/amount will be highest in skin cells
telomerase activity/amount will be highest in brain cells
telomerase activity/amount will be highest in blood cells
telomerase activity/amount will be highest in gametes (sperm/eggs)
telomerase activity/amount will be the same in all of the cell types
Examples of hybridization of single-stranded DNAs used in Chapter 10 include:
primers used in DNA sequencing and in PCR
sticky-end cloning
probes used in Southern and Northern blotting and in diagnosis of mutations
All of the answer options are correct.
Examples of proteins that bind to DNA and then act on it, found in Chapter 10, include:
restriction enzymes
Taq DNA polymerase
DNA ligase
All of the answer options are correct.
Why is ligase needed to make recombinant DNA?
It enables the donor fragments and the vector DNA molecules to initially hybridize.
It is responsible for forming the complementary sticky ends of the donor fragments and the vector DNA.
It is only used when blunt-ended vectors are used to form recombinant plasmids.
It creates phosphodiester bonds at the sites where inserted DNA and vector have attached by complementary base pairing.
It is used in the isolation of recombinant plasmids from those plasmid vectors that did not take up donor DNA.
What would be the immediate consequence in the cloning process if someone forgot to add ligase?
There would be no hybridization between the vectors and the donor DNA fragments.
No complementary sticky ends would form in either the vectors or the donor DNA molecules.
Blunt-ended vectors would be able to form covalent bonds with donor DNA.
The inserted DNA and the vector would be held together only by hydrogen bonds, so recombinant plasmids would lose the inserted DNA.
Recombinant plasmids could not be separated from nonrecombinant plasmids.

In the PCR process, if we assume that each cycle takes five minutes, how many-fold amplifications would be accomplished in one hour?

12-fold
32-fold
512-fold
4096-fold
The position of the gene for the protein actin in the haploid fungus Neurospora is known from the complete genome sequence. If you had a slow-growing strain that you suspected of being an actin mutant and you wanted to verify that it was one, would you (1) clone the mutant by using convenient restriction sites flanking the actin gene and then sequence it, or (2) amplify the mutant sequence by using PCR and then sequence it?
The better choice is to clone and sequence the actin gene to confirm that it has mutated. This is a dependable method provided that suitable restriction sites are available.
The better choice is to amplify the actin gene sequence from the mutant and sequence it to confirm that it has mutated. PCR requires fewer steps, and it can be completed more quickly. However, it requires the production of suitable primers.
Why is it necessary to use a special DNA polymerase (Taq polymerase) in PCR?
because the reaction starts with very small amounts of DNA template
because the polymerase has to be able to remain active after heating to 95 degrees Celsius
because the reaction uses primers which are not usually required for DNA replication
because the amount of template increases with each cycle

One feature that virtually all plasmid vectors have in common is the polylinker (also called a multiple cloning site). This is important because

it helps select transformants from nontransformants.
it carries convenient restriction enzyme sites into which the DNA to be cloned may be inserted.
it allows the vector to replicate independent of host.
All of the answer options are correct.
Virtually all plasmid vectors have a selectable marker. This is important because
it helps select transformants from nontransformants.
it carries convenient restriction enzyme sites into which the DNA to be cloned may be inserted.
it allows the vector to replicate independent of host.
All of the answer options are correct.
Prototrophy is often the phenotype selected to detect transformants. Prototrophic cells are used for donor DNA extraction; then this DNA is cloned and the clones are added to an auxotrophic recipient culture. Successful transformants are identified by plating the recipient culture on minimal medium and looking for colonies. What experimental design would you use to make sure that a colony that you hope is a transformant is not, in fact, a revertant (mutation back to prototrophy by a second mutation in the originally mutated gene) of the auxotrophic mutation?
Use an auxotroph that cannot revert as the recipient, such as one that contains a deletion.
Include a selectable marker on the vector that is added to the auxotrophic recipients. Then use the selection conditions to confirm that the colony is a transformant.
Sequence the gene of interest isolated from a putative transformant to determine if additional mutations have occurred.
All of the answer options are correct.

In: Biology

Calculating Life Insurance There are three common ways that insurance agents can determine the life insurance...

Calculating Life Insurance

There are three common ways that insurance agents can determine the life insurance requirements of an individual: multiple income approach, human life value approach, or needs analysis.

Multiple Income Approach

The simplest way to calculate life insurance is a multiple of the individual’s income, usually five to seven times one’s income. However, one should consider existing properties and other sources of protection (Social Security, pension plans, savings—all discussed in later chapters) that may be included in the portfolio. A person needs to buy protection only for the uncovered balance. Agents also understand the subjective preferences of families: the desire or need to prefer current consumption over future savings, natural optimism, and so forth. These topics are related to complex economic theories that are not handled in this book.

Human Life Value Approach

This method considers your client’s age, gender, occupation, current and future earnings, and employee benefits. There are several steps to determining the overall value of the client if they were to die today:

  • Estimate the client’s earnings from now until a set point in the future — typically their expected retirement age. Be sure to factor in future wage increases as well.
  • Subtract the insured’s annual taxes and living expenses from the total. It’s usually safe to assume 30 percent of their salary will go to taxes.
  • Select an assumed rate of return on the remaining total and subtract it from the gross amount. In other words, subtract the interest you expect the money to earn.
  • Add the cost of additional benefits provided through employment, such as health care, that will need to be replaced when the client dies. Remember to account for inflation.

The primary goal of this method is to replace income lost. It doesn’t necessarily account for funeral costs, children’s educational expenses, or other specific future needs.

Capital Needs Analysis

The capital needs analysis is the most widely-used approach for estimating life insurance coverage. In addition to replacing the client’s salary, it also accounts for other sources of income and the specific needs of survivors.

This method factors in:

  • Current and future income of both the insured and surviving spouse
  • Immediate lump-sum cash needs upon death, such as funeral expenses, debt repayment, and mortgage payoff
  • Future expenses such as college, weddings, long-term care expenses, and retirement funding
  • Existing family assets, retirement funds, or insurance policies

Once all future needs are taken into consideration, there are then two ways to calculate how much insurance the client needs, based on how they want to utilize the funds in the future.

  • Earnings-Only Approach: The survivors will live off only the investment earnings of the policy without cashing in the principal value. This method is preferable if the client wants funds to be available for their children after their spouse has also died. Like any investment, this method is subject to the risk of changing market interest rates. To provide a sufficient income stream, the death benefit is usually significantly higher than in the liquidation approach.
  • Liquidation Approach: The surviving beneficiary utilizes a portion of the principal as well as the investment earnings. There is more risk with this approach, particularly if the investment earns less than originally predicted. The surviving spouse may not have sufficient income to live on for the remainder of their life.

What type of individual or couple would benefit from each of the life insurance calculation alternatives presented above?

In: Accounting

Answer (A) True or (B) False 51. Non-Verbal Communication is used 60% of the time. 52....

Answer (A) True or (B) False 51. Non-Verbal Communication is used 60% of the time. 52. All Feedback is positive in communication. 53. Noise can be only what you hear. 54. The receiver is always responsible for the message. 55. Upward communication is always good for the staff in a organization. 56. There are 4 basic communication styles. 57. Social media allows users to create and share content. 58. Informal communication needs to be written in order to be impactful. 59. When communication flows along official paths in a organization that is bad. 60. Horizontal messages flow between the Supervisor and the staff below. 61. Ethics only applies to the individual, not to the organization. 62. A span of control means you are responsible for 20 to 100 people in the organization. 63. Division of Labor means who does what. 64. Chain of command means who reports to whom and how communication flows. 65. Traditional organizations are mainly upward, informal and not written. 66. Human relations organization is not concerned with social and psychological needs. 67. Human resources is based on people’s desire for a sense of accomplishment. 68. Systems/Contingency organizations contends there is no single best way. 69. Organizational Culture is defined as the patterns of beliefs and expectations shared. 70. The transformational organization is the oldest in the business world. 71. Mutual adjustment involves horizontal communication 72. Learning groups make a series of decisions to solve a problem. 73. Self-maintenance groups seek to inspire desirable attitudes and understanding. 74. Small-group involves a small number of people in face to face interaction. 75. Groupthink is a method for effective means of making decisions. 76. Listening is cognitive. 77. The “Big Five” personality factors are not considered important in successful teams. 78. Task functions must be performed to maintain interpersonal relationships. 79. Maintenance functions encourage, harmonize, relieve tension and gatekeep. 80. Storytelling, confessing, dominating, blocking is a part of dysfunctional behavior. 81. Leadership style has nothing to do with how you handle yourself and others. 82. Authoritarian leadership make decisions and use one-way communication. 83. Democratic leadership style make decisions without followers. 84. Laissez-faire leadership style engages the team to make work assignments. 85. Transformational leaders transform society with their visions. 86. Virtual teams meet face to face often and are in the same location. 87. Situational leadership style has one direct method for delegation. 88. Communication anxiety is never an obstacle to effective communication. 89. Situational anxiety is perfectly normal. 90. Trait anxiety needs no preparation. 91. Social media has no place in the business community. 92. Email is not important to use in a business, it is only for personal use. 93. Sexual harassment is a form of sex discrimination that violates Civil Rights. 94. The Me 2 movement has not impacted business leaders. 95. Nonverbal communication involves only how you look at a person. 96. Monochronic cultures take a laid back approach to time and consider relationships. 97. Polychronic cultures view time as a scarce resource that can be wasted or saved. 98. Listening is not effective for good leaders. 99. The sender is never responsible for the message. 100. Physical, psychological and physiological noise is never in a presentation.

In: Accounting

User Centric Approach CASE STUDY Frontier’s challenge was to deploy flexible, branded, high-performance Wi-Fi and networking...

User Centric Approach CASE STUDY

Frontier’s challenge was to deploy flexible, branded, high-performance Wi-Fi and networking services to commercial subscribers across more than 30 states— rapidly. To this end, reliable, fast Internet connectivity has become a universal expectation. People assume they can be online at small businesses, public buildings, outdoor spaces and venues ranging from airports to arenas, hotels to branch offices, churches to RV parks, and hundreds of other places. Providing seamless connectivity across the full spectrum of work/play/live applications is a major revenue-generating opportunity for Communication Services Providers and Broadband Operators, but only if they can make complexity disappear and shrink time-to-market exponentially.

In order to eliminate the complexity of building its own managed networking and wireless offerings from the ground up, Frontier Business turned to CommScope for a hosted, white label, comprehensive carrier-grade solution. The CommScope Solution: a world-class solution of ready-to-deploy technology and services CommScope was the best way for Frontier to “deliver more, faster.” Its comprehensive, ready-to-go solution includesindustry-leading technology like powerful Ruckus access points, wireless access controllers, ICX switches firewalls and the CommScope Virtual Managed Edge. CommScope helped simplify the entire process, from design, such as developing heat maps for optimal AP locations and switches, through installation and integration. CommScope also managed the on-boarding process, which involved incorporating hardware gear onto the hosted core, and setting up customized user portals and admin dashboards for advanced analytics and reporting.

To eliminate the burden of dealing with different vendors for each component, CommScope manages and monitors the networking platform. Proactive NOC-to-NOC support ensures optimal uptime. In fact, problems are often resolved before the subscriber is even aware of them. CommScope leveraged its unparalleled track record of enabling service provider success, aligning itself to support Frontier’s business objectives. The result was the creation of an expertly coordinated package of service components and software and hardware, tailored to meet Frontier’s specific service performance and budget requirements.

Frontier is in the process of deploying CommScope managed Wi-Fi and managed networking offerings to new and existing commercial customers in multiple market segments across a footprint spanning 28 states. One important goal for the Telecom Provider is to move beyond business relationships that are based on one-time fees, and focus on “stickier” services with monthly recurring revenue. Frontier now has managed Wi-Fi and networking solutions that bring world-class capabilities and competitive differentiation. With CommScope helping to deepen and expand the product portfolio, the door is now open for Frontier to provide new branded offerings, such as cybersecurity services, at a reduced time-to-market.

Answer the following questions based on the case study

1. Discuss the significance of Human Centred (HC) design concept for the company, Frontier, in terms of complexity reduction and shrink time to market exponentially.

2. Analyse in terms of HC design concept why Frontier approached CommScope for its WiFi and networking services over 30 states.

3. How has Frontier benefitted from CommScope design? Illustrate in light of the human-centred design concept.

In: Computer Science

QUESTION – User Centric Approach CASE STUDY Frontier’s challenge was to deploy flexible, branded, high-performance Wi-Fi...

QUESTION – User Centric Approach CASE STUDY


Frontier’s challenge was to deploy flexible, branded, high-performance Wi-Fi and networking
services to commercial subscribers across more than 30 states— rapidly. To this end, reliable,
fast Internet connectivity has become a universal expectation. People assume they can be
online at small businesses, public buildings, outdoor spaces and venues ranging from airports
to arenas, hotels to branch offices, churches to RV parks, and hundreds of other places.
Providing seamless connectivity across the full spectrum of work/play/live applications is a
major revenue-generating opportunity for Communication Services Providers and Broadband
Operators, but only if they can make complexity disappear and shrink time-to-market
exponentially.
In order to eliminate the complexity of building its own managed networking and wireless
offerings from the ground up, Frontier Business turned to CommScope for a hosted, white
label, comprehensive carrier-grade solution. The CommScope Solution: a world-class solution
of ready-to-deploy technology and services CommScope was the best way for Frontier to
“deliver more, faster.” Its comprehensive, ready-to-go solution includesindustry-leading
technology like powerful Ruckus access points, wireless access controllers, ICX switches
firewalls and the CommScope Virtual Managed Edge. CommScope helped simplify the entire
process, from design, such as developing heat maps for optimal AP locations and switches,
through installation and integration. CommScope also managed the on-boarding process,
which involved incorporating hardware gear onto the hosted core, and setting up customized
user portals and admin dashboards for advanced analytics and reporting.
To eliminate the burden of dealing with different vendors for each component, CommScope
manages and monitors the networking platform. Proactive NOC-to-NOC support ensures
optimal uptime. In fact, problems are often resolved before the subscriber is even aware of
them. CommScope leveraged its unparalleled track record of enabling service provider
success, aligning itself to support Frontier’s business objectives. The result was the creation
of an expertly coordinated package of service components and software and hardware,
tailored to meet Frontier’s specific service performance and budget requirements.

Frontier is in the process of deploying CommScope managed Wi-Fi and managed networking
offerings to new and existing commercial customers in multiple market segments across a
footprint spanning 28 states. One important goal for the Telecom Provider is to move beyond
business relationships that are based on one-time fees, and focus on “stickier” services with
monthly recurring revenue. Frontier now has managed Wi-Fi and networking solutions that
bring world-class capabilities and competitive differentiation. With CommScope helping to
deepen and expand the product portfolio, the door is now open for Frontier to provide new
branded offerings, such as cybersecurity services, at a reduced time-to-market.


[Adapted form https://www.commscope.com/globalassets/digizuite/62453-frontier-
managed-networks-cs-113957-en.pdf]

Answer the following questions based on the case study
1. Discuss the significance of Human Centred (HC) design concept for the company,
Frontier, in terms of complexity reduction and shrink time to market exponentially.

2. Analyse in terms of HC design concept why Frontier approached CommScope for its
WiFi and networking services over 30 states.

3. How has Frontier benefitted from CommScope design? Illustrate in light of the
human-centred design concept.

In: Computer Science

1          According to evolutionary theory, organisms with very similar phenotypes: a.         are always very closely related...

1          According to evolutionary theory, organisms with very similar phenotypes:

a.         are always very closely related

b.         always have very similar genotypes

c.         will evolve under very similar conditions.

d.        can have very different genotypes.

e          both c and d are correct.

2          Which of the following is NOT supported by scientific evidence?

a.      Probability Theory

b.      Evolutionary Theory

c.      Genetic Theory

d.      Cell Theory

e.      all of the above ARE scientific theories supported by large amounts of evidence

3.         At the beginning of western settlement millions of buffalo lived on the Great Plains. By 1890 only about 750 survived in the entire country. This is an example of:

a.         a severe mutation

b.         negative gene flow

c.         the founder effect

d.         a genetic bottleneck.

4.         Livestock production in the U.S. today uses nearly 3 times as many antibiotics as are prescribed for all human patients. It is most likely that routine use of antibiotics in animal feed will result in:

a.         an effective, long term way to eliminate disease-causing bacteria in the animals and therefore increase food production.

b.         the animals developing resistance to the antibiotics.

c.         an increased number of antibiotic-resistant bacterial strains.

d.         the elimination of viral infections and thus increased food production.

e.         ever fewer disease-causing bacteria in the environment.

5.         Roaches are a common indoor pest in the south. Why must people routinely change the brand of insecticide (roach killer) they use?

a.         The active ingredients in the insecticide break down over time.

b.         Antibiotic-resistance genes in roach DNA are transferred to uninfected roaches by contact with infected ones.

c.         The direct action of insecticides on roach DNA cause individual roaches to slowly become immune.

d.         Some individual roaches in the population may have a mutation providing some natural immunity to the first insecticide and their offspring will begin to predominate.

e          Changes in the chemical formulation of insecticides constantly improve their effectiveness.

6.         Both Darwin and Wallace were greatly influenced by Thomas Malthus who observed that

            a.         populations increase geometrically (exponentially).

            b.         populations increase at the same rate as the food supply.

            c.         populations remain at their carrying capacity.

            d.         food supply usually increases faster than the population that depends on it.

7.         Examples of adaptive radiation of species would be easiest to find:

a.         near nuclear power plants.

b.         in isolated regions with many diverse habitats.

c.         on mountain tops exposed to higher UV radiation.

d.         in moist, humid tropical rainforests.

e.         in areas with much human contact.

8.         Gene flow is an evolutionary factor associated with:

a.         artificial selection

b.         random mating

c.         increased mutations

d.         migration of individuals

e.         small populations

9.         Evolution occurs in:

a.         gametes.

b.         somatic cells.

c.         individuals.

d.         organelles.

e.         populations.

10.       English moths originally had a normal (bell-curve) distribution phenotypes. What was the result of the smoke and soot from the industrial revolution on those English moths?

a.         All phenotypes in the original population were reduced as the population declined.

b.         Both extremes of the curve were selected against and the curve became narrow and tall.

c.         Heterozygote individuals were weaker and died off.

d.         The entire population of moths became darker but retained the bell-shaped curve.

e.         The moth population went extinct.

In: Biology

Business and Data Management Module 3 Case Study One Instructions Scenario: You have been hired as...

Business and Data Management

Module 3

Case Study One Instructions

Scenario:

You have been hired as a security analyst for Garbo Rheumatology Research Center. This lab is producing cutting edge treatments for rheumatology and similar autoimmune diseases. Despite being cutting edge, this research is not top secret. Funding is provided by government grants and sponsored by a major research university in the area.

While there is not an intense amount of security required, the data being researched does include medical and laboratory data taken from human and animal subjects. This information comes from a small, attached, on-site clinic and several small animal labs on university premise. Currently, accessibility to the facilities employ appropriate physical controls to ensure that only authorized individuals are permitted within the given facilities; via a combination of security badges and pin codes.

Laboratory

The labs are dedicated to research materials. Lab technicians utilize three shared desktops located in the rear of the labs to register their data. They use separate logins and are required to log out after they complete their work. Many of these technicians are Ph.D. candidates or post doctorate students who, also, utilize these computers to correspond with their advisors. Some of these advisors are the researchers who work on projects within the labs, while others are professors on the university’s main campus.

The actual data itself is stored in a server room, which also functions as the office of the systems administrator. Access to this room is available only to the systems administrator and the Primary investigator (PI) of the project. The medical doctors/researchers, who work on site, each have a private office where they review and analyze the research data. Each office is accessible only to the occupant and building maintenance. These researchers deal with patient medical records, student coursework, and research data produced by the lab.

Data held in the database -

  • Medical patient profile
  • Medical test results
  • Demographic information of Individual patients
  • Clinical visits organized by patient

Paperwork spread around the office -

  • Physical version of patient’s medical chart
  • Printouts of test results and biological samples from both human patients and animal subjects
  • Student assignments with and without grades

Medical Clinic

The medical clinic is permanently staffed with receptionists, office staff, and nurses. The medical doctors/researchers rotate shifts seeing patients and do not have permanent office space in this area. Instead they share desks and computers, while the nurses and office staff have permanent space and computers assigned to them individually. There is a shared workspace at the front desk which is utilized for patient booking, patient sign-in, and processing patient payments. Patients pay for appointments with credit and debit cards.

Data found in the Clinic –

  • Patient logs (sign-in sheets)
  • Printouts of credit card transactions
  • Receipts left by patients
  • Medical records of patients being seen that day
  • New patient profiles

Assignment:

Part of evaluating the security of an organization is understanding the environment which the business operates. It is important not to just identify the data being handled, but, also, to understand what laws and regulations protect the data. This information might force us to handle and protect the data in ways that we would not otherwise have planned to in order to comply with the regulations.

  • Based on the above scenario, list in “table format” the laws and regulations that this organization should consider in terms of welfare and data privacy best practices? There are numerous laws and regulations available to research.
  • Based on these standards (i.e., HIPAA), what best practices would you suggest for healthcare compliance requirements?

In: Computer Science