Describe how would you prepare the GTE (50 mM Glucose, 20 mM Tris, EDTA) Resuspension Solution for use in a mini-prep. You have the following stock solutions: 0.5 M glucose, 0.2 M Tris-HCl (pH 8.0), 0.5 M EDTA (pH 8.0).
How would you prepare 150 mL of GTE re-suspension solution using the above stocks?

Also, describe how to prepare the solution in appropriate container and aseptic technique.

A maximum of 10% of the energy present in any trophic level is passed on to the next level when an organism from the lower level is consumed by an organism from the higher level.  Imagine that an acre of land produces 50 pounds of plant biomass per week.  Calculate how many acres of land would be required to support a tertiary consumer (some type of carnivore) that consumes 10 pounds of prey per week.

Nuclear Fuel Cycle:

Assuming that the price per SWU is $80 and the cost of conversion is $4/kgU, what is the
price of the U3O8 ($/lb U3O8) beyond which it will cost less to enrich the already mined, purified, and
converted (to UF6) tails that contain 0.2% U-235 rather than mine new uranium? Assume the product
will be 3% enriched in U-235 in either case and the new tails will be 0.1% (when the old tails are
enriched). Tails stored as UF6 cost nothing.

Nurses Pay is $80K/year and Physician Pay is $300K/year. The clinic is running with 2 physicians at a physician to nurse factor input ratio of 0.2. However, there is an increase in physician pay to $330K/year. The firm will now run with 1 physician with the physician to nurse factor input ratio of 0.072. In addition, please also calculate savings with the new factor input ratio. Then calculate the elasticity of substitution. What can you say about the type of service provided by this clinic? Please explain.a

$200,000 house at 5.0% interest, 30 year loan, the LTV is 90%. Assume that the PMI has an upfront premium of 2% and an annual premium of 0.2%.

1. How much is the upfront PMI payment and how much is the monthly PMI payment?

2. How many months you have to pay PMI (when can you call your lender to request drop PMI payment)?

3. How many months your lender have to drop your PMI payment?

A pharmaceutical product is crystallized and washed with absolute ethanol. A 100 kg batch of product containing 10% ethanol by weight is to be dried to 0.1% ethanol by weight by passing 0.2 m3/min of 50�C nitrogen through the dryer. Estimate the rate (mol/min) that ethanol is removed from the crystals, assuming that ethanol exerts the same vapor pressure as if it were pure liquid. Based on this assumption, estimate the residence time for the crystals in the dryer. The dryer operates at 0.1 MPa and the vapor pressure of the pharmaceutical is negligible.

5. Suppose f(1) = 2, g(1) = −1, f′(1) = 3, g′(1) = 2, f(2) = 1, and f′(2) = 0.2. Calculate the derivatives of the following functions at the provided point (be careful with using the correct values) 3 pts each:

   (a) d/dx (e^xf(x)) when x=2

   (b) d/dx (f(x)/g(x)) when x=1

   (c) d/dx (ln(xf(x))) when x=1

Your classmate finds a bottle simply marked HCl in the laboratory and decides to conduct an acid-base titration using a pH meter. They add 0.2 N sodium hydroxide (NaOH) to 25 mL of the HCl solution. The next day another lab-user tells you that they prepared the HCl solution in question and that it has a molarity of 0.15 M. What may explain the discrepancy between the experimental and theoretical values?

From the curve, pH at equivalence point is 7 and Volume of NaOH= 22ml

An enzyme was obtained in Dr. Cai’s lab. To study the kinetics of this enzyme, a

graduate student measured the velocity of the reaction at a substrate concentration

of 0.2 mM substrate. He found the initial rate decreased to 1/3 after 1.2 mM

competitive inhibitor was added. KI, the dissociation constant for the inhibitor

binding to the enzyme, is 0.5 mM. What is the KM, the Michaelis constant?

A. 0.33 mM
B. 0.5 mM
C. 0.1 mM

D. 1 mM

E. 10 mM