You plan on isolating a protein of interest by cutting a band out of a gel. Your protein is a phosphatase (a certain type of enzyme) and you hope to measure its activity. Should you use SDS-PAGE or a native gel to isolate your protein? Why?

How will you describe and contrast on enzyme activity based on Km and Vmax from Michaelis menten equation? (low or high Km/Vmax)
what does it mean when Km is High or low
what does it mean when Vmax is High or Low

4. List two properties of ammonia that cause problems for cells trying to acquiring nitrogen. (Don’t just say ammonia is too scarce in the environment) (1) _______________________________ (2) ______________________________ Describe in general terms how the cell solves this nitrogen acquisition problem (no enzyme names): ____________________________________________

Hi! I'm doing a lab report on the synthesis of divanillin from vanillin and h202 using horseradish peroxidase type 1 enzyme. I'm wondering what the steps are in the reaction (drawing), and what the roles of h202, acetic acid, and ph changes are? :) Thank you!

describe two enzymes found in the mouth, two enzymes found in the stomach, and two enzymes found in the small intestines and describe one organic compound that each enzyme breaks down.....i need a good and understandable explanation , answer for this question ,thank u

1.In order for the electron transport chain and ATP synthase to function, the inner membrane of the mitochondrion has to be impermeable to most solutes, especially ions. Why?

2.Would you expect a competitive or a noncompetitive inhibitor to be more similar to the natural substrate of an enzyme? Why?

1. Discuss the composition and structure of enzymes and discuss the factors that modify enzyme structure and function

2. Describe the production and processing of a protein that will be exported from a eukaryotic cell. Begin with the separation of the mRNA from the DNA template and end with the release of the protein at the plasma membrane.

One molecule of glyceraldehyde-3-phosphate is fed into glycolysis. Which and how many electron carrier molecule(s) are made during (A) the glycolytic breakdown of this molecule, during (B) acetyl-CoA production and during (C) the citric acid cycle? (D) How many H⁺ are translocated across the mitochrondrial membrane due to the re-oxidation of these electron carriers? Write the numbers into the respective boxes.

This question is very important for me to understand. Please only respond if you are answering all parts and are confident they are correct. Thank you!

Under anaerobic conditions, pyruvate is converted into lactate in vertebrate muscle tissue. This conversion allows the muscles to continue carrying out glycolysis because

Group of answer choices The decarboxylation of pyruvate to lactate regenerates NAD+, which maintains redox balance. The reduction of pyruvate to lactate regenerates NADH, which maintains redox balance. None of the other choices is correct. The decarboxylation of pyruvate to lactate regenerates NADH, which maintains redox balance. The reduction of pyruvate to lactate regenerates NAD+, which maintains redox balance.

Trypanosomes living in the bloodstream obtain all their free energy from glycolysis. They take up glucose from the host’s blood and excrete pyruvate as a waste product. In this part of their life-cycle, trypanosomes do not carry out any oxidative phosphorylation, but they do use another oxygen dependent pathway, which is absent in mammals, to oxidize NADH.

Why is this other pathway necessary?

Would the pathway be necessary if the trypanosomes excreted lactate rather than pyruvate?

Why would this pathway be a good target for antiparasitic drugs?