Questions
One enzyme-catalyzed reaction in a biochemical cycle has an equilibrium constant that is 10 times the equilibrium constant of a second reaction

One enzyme-catalyzed reaction in a biochemical cycle has an equilibrium constant that is 10 times the equilibrium constant of a second reaction. If the standard Gibbs energy of the former reaction is -300 kJ/mol, what is the standard reaction Gibbs energy of the second reaction?

In: Chemistry

Consider the reaction that converts pyruvic acid (CH3COCOOH) into gaseous acetaldehyde (CH3CHO) and gaseous CO2, which...

Consider the reaction that converts pyruvic acid (CH3COCOOH) into gaseous acetaldehyde (CH3CHO) and gaseous CO2, which is catalyzed in aqueous solution by the enzyme pyruvate decarboxylase. Assume ideal gas behavior for CO2.

Calculate ?rG (298 K) for this reaction at 160 bar.

In: Chemistry

Create DICHOTOMOUS KEY showing which selective media and biochemical tests you would need if you were...

Create DICHOTOMOUS KEY showing which selective media and biochemical tests you would need if you were to test your unknown bacteria (SALMONELLA ENTERICA) in the lab and also, describe the dichotomous key of Salmonella Enterica in paragraph like in example shown below.

[[ Like this is example for Staphylococcus epidermis shown by my professor: You would say this. According to my unknown bacterium 16S DNA sequence result from NCBI database, my unknown bacterium was identified to be Staphylococcus epidermidis. To identify and confirm Staphylococcus epidermidis bacterium in lab traditionally using culture and media, I would first grow the bacterium in nutrient broth, then using the grown culture, I would perform Gram Staining, which should show me purple cells under microscope because this bacterium is gram-positive, then I will procced to testing for Catalase enzyme. This test would help me differentiate Staphylococcus from Streptococcus. The catalase test should be positive. Then, I will test for coagulase enzyme to make sure it is negative because coagulase positive would indicate my bacterium is Staph aureus. Then, I would test for oxidase enzyme to make sure it is negative. Positive oxidase enzyme would indicate my bacterium is Micrococcus. Then, I would perform Kirby-Baur assay using novobiocin antibiotic to test for the antibiotic sensitivity. The larger zone of inhibition means the bacterium is sensitive to novobiocin, which indicates the the bacterium is Staphylococcus epidermidis.

The selective media we can use to ID Staphylococcus Epidermis is the MSA (Mannitol Salt Agar).]]

Like wise in the same way, as above example for Staphylococcus Epidermis, 1) Create DICHOTOMOUS KEY showing which selective media and biochemical tests you would need if you were to test your unknown bacteria (SALMONELLA ENTERICA) in the lab and 2)Describe the dichotomous key of Salmonella Enterica in paragraph like in example shown.

In: Biology

Suppose that you have isolated the enzyme sucrase (able to hydrolyze sucrose into glucose and fructose),...

Suppose that you have isolated the enzyme sucrase (able to hydrolyze sucrose into glucose and fructose), and you wish to determine the nature of inhibitor A for this enzyme. You have prepared five different concentrations of substrate (sucrose), and five different concentrations of inhibitor A (plus the control, with zero mM of inhibitor A). The following Table lists the inhibitor A concentrations [I], substrate concentrations [S], and resulting enzyme velocities (Vo) for all six of these experiments:

[I]

[S]

Vo

1/[S]

1/ Vo

0 mM

0.1 mM

0.333333333333 mM per minute

0 mM

0.2 mM

0.50

0 mM

0.3 mM

0.60

0 mM

0.4 mM

0.666666666667

0 mM

0.5 mM

0.714285714286

0.1 mM

0.1 mM

0.20

0.1 mM

0.2 mM

0.333333333333

0.1 mM

0.3 mM

0.428571428571

0.1 mM

0.4 mM

0.50

0.1 mM

0.5 mM

0.555555555556

0.20 mM

0.1 mM

0.142857142857

0.20 mM

0.2 mM

0.25

0.20 mM

0.3 mM

0.333333333333

0.20 mM

0.4 mM

0.40

0.20 mM

0.5 mM

0.454545454545

0.3 mM

0.1 mM

0.111111111111

0.3 mM

0.2 mM

0.20

0.3 mM

0.3 mM

0.272727272727

0.3 mM

0.4 mM

0.333333333333

0.3 mM

0.5 mM

0.384615384615

0.40 mM

0.1 mM

0.090909090909

0.40 mM

0.2 mM

0.166666666667

0.40 mM

0.3 mM

0.230769230769

0.40 mM

0.4 mM

0.285714285714

0.40 mM

0.5 mM

0.333333333333

0.5 mM

0.1 mM

0.076923076923

0.5 mM

0.2 mM

0.142857142857

0.5 mM

0.3 mM

0.20

0.5 mM

0.4 mM

0.25

0.5 mM

0.5 mM

0.294117647059

Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Vmax/Km ) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor A ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor A?

Since many of you do not have scanners or cameras to send me an e-mail image of your graphs, the grading of this assignment will be based entirely on your calculations. I will be looking for your six Vmax calculations (6 points), your six Km calculations (6 points), your six Vmax/Km or slope of the line calculations (6 points), and the type of reversible enzyme inhibition that you identify for inhibitor A, along with reasons to justify your identification (2 points).

In: Biology

What do you perceive are the different roles and activities commonly required for human resource managers?...

  1. What do you perceive are the different roles and activities commonly required for human resource managers?
  2. How are human resources managerial roles and activities affected by aspects of leadership planning and compensation?
  3. In your opinion is it important for human resources managers to be certified through the Society of Human Resource Management? Explain your stance.

In: Economics

Carboxylic acids can be made by the hydrolysis of nitriles, which in turn can be made from an alkyl halide.

Carboxylic acids can be made by the hydrolysis of nitriles, which in turn can be made from an alkyl halide. Draw the structures of a starting alkyl bromide and the intermediate nitrile that would be used in the synthesis of 3-methylbutanoic acid (isovaleric acid) Do not show free ions.

In: Chemistry

Describe the relationship between chemical equilibrium and the binding of hemoglobin to oxygen and explain why...

Describe the relationship between chemical equilibrium and the binding of hemoglobin to oxygen and explain why strong acids are good conductors of electricity and weak acid are not

please help me and explain in detail so i can understand.( preferably give examples for both questions) thanks! :)

In: Chemistry

7. Polysaccharides play an important role in the cell walls of prokaryotes such bacteria and of...

7. Polysaccharides play an important role in the cell walls of prokaryotes such bacteria and of certain eukaryotes like fungi and plants. Give 3 reasons why polysaccharides as opposed to other biomolecular polymers (fats, proteins, nucleic acids) were better suited to form these walls.

In: Chemistry

Because the oxide ion is basic, metal oxides react readily with acids. (a) Write the net ionic equation for the following reaction:

Because the oxide ion is basic, metal oxides react readily with acids.

(a) Write the net ionic equation for the following reaction:

FeO(s) + 2 HCIO,(aq) → Fe(CIO,)2(aq) + H20(1)

 

(b) Based on the equation in part (a), write the net ionic equation for the reaction that occurs between NiO(s) and an aqueous solution of nitric acid.

In: Chemistry

Identify the following as acids, bases, or neutral solutions: contains more hydronium ions than hydroxide ions...

Identify the following as acids, bases, or neutral solutions:

contains more hydronium ions than hydroxide ions

H2O

[H3O+] = 3.4 × 10-5 M

[OH-] = 2.8 × 10-2 M

Ca(OH)2

[H3O+] = 1.0 × 10-7 M

In: Chemistry