1- Why is the FRAP technique applicable only to live cells?

2- A researcher wishes to confirm that the adenine nucleotide translocase (ANT) is localized to mitochondria by using immunofluorescence microscopy. He has an anti-ANT antibody fluorescently labelled with Alexa Fluor red and plans to show co-localisation with mitochondria using mitotracker red. Why will this not work? Suggest a better approach.

3- Briefly describe the principle of the FISH technique and indicate one type of sample that this technique could be used with.

4- You are working in a lab that had access to a range of deletion collections. A company approaches your supervisor with a new drug that has great anti-tumor properties and also inhibits the growth of yeast. The company wants to check that the mechanism of action of the drug is novel. That is, it is not similar to other known anti-tumour compounds. what techniques could you use to:

a. Demonstrate the drug has a different mechanism of action from known anti-tumor compounds.

b. Elucidate the mechanism of action of the drug

You are a researcher at a small biotech company and your company has just obtained the license for use of a human GENOMIC DNA fragment putatively encoding a potentially novel protein, which is thought to regulate p53, the known tumor supressor protein. The scientists who originally cloned this GENE fragment HDM5 "claim" that HDM5 shares 90% DNA sequence homology with one of the HDM2 genes (refer to the review Levine & Oren, 2009). They propose that HDM5 may have HDM2-like properties and may be involved in regulating cell proliferation, and thus a good target to potentially develop as a cancer therapy. Your company has asked you to characterize the gene and gene products, as well as to provide an opinion as to its potential human therapeutic uses.

1. Before proceeding, you wish to know whether this gene is actually expressed in humans ?Describe, in detail, 2 efficient experimental approaches to answer this question. ?Discuss whether your proposed methods measure synthesis or accumulation of the product

Find a poet who appeals to you from a culture other than your own in our text Compact Literature. Why does his or her work appeal to you? Explain your ideas and feelings so that your colleagues can understand why you have chosen this particular author. *Note that the poet that you choose to discuss in this posting should come from a culture that is different from your own. You need to explain those differences and how they are reflected in the poet’s work. You will have to do a little research for this particular discussion posting, but you can also use your textbook to help you. Make sure to include a specific poem from this poet and explain why you like it. Once you have examined the cultural elements of the chosen poem, ask yourself: how does the culture in Achebe’s novel impact my understanding of the story? Does your chosen poet bring new meaning to his/her culture? …to your culture?

1. Using your knowledge of cell & molecular biology techniques, design an experiment to investigate a novel hypothesis for the process of vesicle transport. The hypothesis proposes that a network of defined microfilament tracts in the cytoplasm exist (like railroad tracks!) and that are not visible under a microscope and that do not stain with the regular histochemical dyes that are available. These tracts are of distinctly different types and are made up of proteins – each type of unique tract specifically interacts with specific and unique type of target vesicles and guides them from ER to their final destination (eg., lysosomes, membrane, secretory, etc.).

a. Provide a specific strategy (design) of an experimental plan to investigate this hypothesis with appropriate rationale.

b. Experimental Plan – provide specific details – separate them using sub-topics if a multiple step plan is used. Describe: Cell & Molecular Approach Reagents used Instrumentation used

c. Observations & Results Expected

d. Make – pros and cons – if a approach is challenging – explain why and provide a discussion.

e. Conclusion

(a) According to the Ministry of Health, the height of Guinean travellers who were quarantined in Tamale for the novel coronavirus were normally distributed about a mean of 160cm and a standard deviation of 8cm. Find the probability that a traveller selected at random has
i) Height between 148cm and 175cm                                                                        ii) Height above 164cm                                                                                               iii) Height below 179cm                                                                                              
b) The average time taken for the nearest Covid-19 ambulance team to convey a patient showing symptoms to the nearest designated hospital follows the normal distribution about a mean of 60 seconds and a standard deviation of 8 seconds. If 200 patients were selected at random, how many of them could be taken to the hospital in
i) Less than 50 seconds                                                                                             ii) More than 64 seconds                                                                                           
c) The Shelf life of a particular dairy product is normally distributed with a mean of 12 days and a variance of 9 days. About what percentage of the products last between 13 and 16 days
                                                                                                                                                             
                                                                                                                                                         [Total: 20 Mark

a) According to the Ministry of Health, the height of Guinean travellers who were quarantined in Tamale for the novel coronavirus were normally distributed about a mean of 160cm and a standard deviation of 8cm. Find the probability that a traveller selected at random has
i) Height between 148cm and 175cm                                                                        ii) Height above 164cm                                                                                               iii) Height below 179cm                                                                                              
b) The average time taken for the nearest Covid-19 ambulance team to convey a patient showing symptoms to the nearest designated hospital follows the normal distribution about a mean of 60 seconds and a standard deviation of 8 seconds. If 200 patients were selected at random, how many of them could be taken to the hospital in
i) Less than 50 seconds                                                                                             ii) More than 64 seconds                                                                                           
c) The Shelf life of a particular dairy product is normally distributed with a mean of 12 days and a variance of 9 days. About what percentage of the products last between 13 and 16 days
                                                                                                                                                             
                                                                                                                                                         [Total: 20 Mark

Please work on the assignment below: Please review the instructions

INTEGRATIVE LEARNING ASSIGNMENT

Writing about Statistics

• Choose one of the following Health issues (migraine cures, cancer cures, the spread of malaria, pediatric health issues, blood pressure medicines, controversial or novel techniques for curing illnesses….)
• Write the General summary of the article including the type of study discussed
• Statistics involved in the topic (be very detailed)
• Technical details or ne points about the topic
• Conclusion, including your opinions about the study and why this article is important to you

You will write a detailed summary of the article, addressing all the following:

How do I proceed?

STEP 1 Find an article.

Cite the reference to the article of your choice. If you used a print magazine/paper, enter the name of the source.

STEP 2 Write your detailed summary of the article addressing the criteria listed above. Give meaningful title to your paper. Limit the length of your paper to 1000 words.

The chapter discussed the Internet as a disruptive innovation that has facilitated online retailing. It also, however, has presented challenges to brickand-mortar retailers. How might retailers such as Nordstrom, Neiman Marcus, or Macy’s need to change their in-store experience in order to continue to attract a flow of customers into their stores to expand sales using direct selling and
store displays of the actual merchandise? If the Internet continues to grow and sales of brick-andmortar retailers decline, how might the retailers attract, train, and retain high-quality employees if the industry is perceived as in decline?

b. Much has been said about competitive advantage gained from innovations such as the Internet, hightechnology gadgets, and apps. The chapter points out, however, that low-technology innovations such as the razor–razor-blade business model can also create value. Think of other low-technology innovations that are/were novel, useful, and successfully implemented so that the innovating firm gained a competitive advantage. Find information about the entrepreneurial story behind the innovation.

The world post Covid 19 has created the concept of social distancing which will impact on the way we do business. Online purchasing is expected to increase because it will reduce human interaction and contribute to the reduction of Covid 19 related illnesses. However, online purchases have created contemporary legal issues which may be a novel for most legal systems in several Caribbean Countries.

You are required to

critically discuss the concept of online purchases and the various methods used in online transactions with supporting case laws. Secondly, critically discuss FIVE (5) CONTEMPORY CASE LAWS related to online purchasing at the International Level, focusing on the judgements handed down in those cases and most importantly how do you think that will impact on the Caribbean online purchaser. Finally, bearing in mind your answers above; what do you think (your opinion) that Caribbean Governments can initiate via its legal systems to enhance and protect online purchasers legal rights?

The world post Covid 19 has created the concept of social distancing which will impact on the way we do business. Online purchasing is expected to increase because it will reduce human interaction and contribute to the reduction of Covid 19 related illnesses. However, online purchases have created contemporary legal issues which may be a novel for most legal systems in several Caribbean Countries.

You are required to critically discuss the concept of online purchases and the various methods used in online transactions with supporting case laws.

Secondly, critically discuss FIVE (5) CONTEMPORY CASE LAWS related to online purchasing at the International Level, focusing on the judgements handed down in those cases and most importantly how do you think that will impact on the Caribbean online purchaser.

Finally, bearing in mind your answers above; what do you think (your opinion) that Caribbean Governments can initiate via its legal systems to enhance and protect online purchasers legal rights?