Kinetic Properties of a Key Enzyme of Prostaglandin Synthesis

Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. The enzyme converts arachidonic acid to PGG₂, the immediate precursor for many prostaglandins. The table below show the result of enzyme activity with or without an inhibitor, ibuprofen.

You need first to determine the V_max and K_m, with and without the inhibitor, and then determine which type of inhibitor Ibuprofen is.   The data given here makes it does not afford convenient assessment of V_max and K_m by conventional Michaelis Menten graph (since saturation is not reached under the experimental conditions). Therefore, a Lineweaver-Burke analysis is required. Fill the table with the reciprocal values. The results then are to be drawn as a non-logarithmic graph (next page).

Please show the equation on how to find the 1/[s], the 1/Vo and the 1/Vo[Ib] in the chart. Thank you

Calculate the pI of ASN (α-carboxyl group pK a= 2.1; : α-amino group pK a=9.0

When Da Vinci's first airplane model was sold at auction in 1895, it went for $230. In 2016, the original owner sold the paininting again and this time Sotheby's auctioneers were able to sell it for the staggering sum of $1,450,000.

a. What was the annual percentage increase in price of the painting over this period? (Do not round intermediate calculations and enter your answer as a percent rounded to 2 decimal places, e.g., 32.16.)

b. How much money should the painting be worth in 2030 if the price of the painting increases at the same rate as (a)? (Do not round intermediate calculations and round your answer to 2 decimal places, e.g., 32.16.)

da Sidha Karya Company is a family-owned company located on the island of Bali in Indonesia. The company produces a handcrafted Balinese musical instrument called a gamelan that is similar to a xylophone. The gamelans are sold for $990. Selected data for the company’s operations last year follow:

The absorption costing income statement prepared by the company’s accountant for last year appears below:

Required:

1. Under absorption costing, how much fixed manufacturing overhead cost is included in the company's inventory at the end of last year?

2. Prepare an income statement for last year using variable costing.

DA Inc. is currently an all-equity firm, with a value of $500. It has 25 shares outstanding. The EBIT is 153.85 per year forever. The tax rate is 35%. The payout is 100%. It is planning to do a capital restructuring by issuing $200 of perpetual debt, costing 10% and use the proceeds to repurchase stock.
- What is the cost of unlevered equity?
- How many shares will it repurchase? At what price?
- What is the cost of levered equity? Confirm your answer by both computing the PV of all cash flows to shareholders at levered equity cost and using MMII.

  Explain whether you prefer the representation of the Last Supper by Tintoretto, Veronese, or Leonardo da Vinci, identifying specific elements of the Mannerist and Renaissance artistic styles.

This part is easy,

I prefer ______________who is a _____________________ artist.

                                                          Renaissance or Mannerist

The two elements of the _____________________ artistic styles that are

                                             Mannerist or Renaissance

interesting to me are 1_________________ and 2________________ and why?

De Vinci is a Renaissance painter-Tintoretto& Veronese are Mannerist painters

Discuss whether you would have sided with Veronese or with Inquisition court, considering the issues of artistic freedom, a right of the patron, and appropriate subject matter. Provide a rationale for your response.

This is easy too. I would have sided with _________________________

Because of His/their views on:

Artistic Freedom which says_________________________________

Right of the Patron which says__________________________________

Appropriate subject Matter which says__________________________

This last one is easy to describe…….

Describe a real modern situation where similar issues arise, like a controversial film, a professor with controversial views, a business owner expressing a religious opinion in public, a business owner who has an employee who is "just different," etc.

The situation you are describing is it:
Artistic Freedom

A right of the Patron

Appropriate subject Matter

​

Module 05

1. What is the definition of metabolism?

2. The breaking down of body compounds is known as what?

3. True or False: Amino acids becoming protein is an anabolic reaction.

4. What is the sugar that helps make up ATP?

5. From which B vitamin is CoA, or coenzyme A, derived?

6. What are the features/characteristics of aerobic metabolism?

7. For short, intense exercise, which energy-producing pathway does the body rely on most?

8. Anaerobic means ________.

9. When a person performing intense physical exercise begins to feel fatigue and a burning pain in the muscles, the muscles respond by synthesizing more of what substance? (Lactate vs. glucose vs. fatty acids?)

10. True or False: Low carbohydrate diets induce ketosis

11. An individual is described as ________ when they have the endurance to engage in daily activities, as well enough reserve energy to handle added challenges.

12. A minimum of ______ minutes at a time of aerobic physical activity is recommended per activity session.

13. ________ refers to an increase to the size and strength of muscles in response to use.

14. True or False: Putting a demand on muscles repeatedly by making them work harder is the most effective, safe way for athletes to add muscle tissue.

15. According to ACSM guidelines, resistance or strength exercise should be incorporated on ________ per week.

16. An athlete getting ready to compete in the 100-meter dash will rely primarily on what substance for quick energy in this event? (Fats vs. lactate vs. creatine phosphate?)

17. The body’s primary source of glucose during physical activity is ________.

18. True or False: With regards to the energy systems and fuels used by the body, all energy systems are used at all times, but one system which dominates depending on intensity and conditioning of athlete.

19. What type of diet does research suggest can provide endurance athletes with ability to go longer without fatigue?

20. ________ are the dietary nutrients most effective at raising muscle glycogen concentrations.

21. Activities lasting longer than 20 minutes will gradually start using more ________ for fuel and less ________.

22. True or False: Consuming a sports beverage to obtain carbohydrates is recommended during exhausting endurance activities lasting more than 1 hour.

23. The phenomenon known as “hitting the wall” occurs in athletes when ________ is depleted.

It’s recommended that an athlete

• 1: classify the below list as Strong acids    Weak acids Strong bases Weak bases

• HNO2

• HCl

• LiOH
• HClO4
• Ca(OH)2
• NH3
• HCN
• Na3PO4
• 2:classify as forward or reverse favored reaction