How will I find the pI for a non-ionizable amino acid?

For each of the following tRNA anticodon sequences, determine which amino acid would be charged onto the tRNA. (note: these are ANTIcodon sequences, not codon sequences) If more than one tRNA is necessary to recognize all codons for that amino acid, state the anticodon sequences of the other tRNAs that would also be charged with that amino acid. If a given sequence is not a possible tRNA anticodon sequence, explain why not.

A. 5’- IAU - 3’

B. 5’- GCC - 3’

C. 5’- CCG - 3'

D. 5’- UAU - 3’

E. 5’- AGG - 3’

F. 5’- ICA - 3’

Now, for any one of the possible tRNAs, sketch a ribosome with the charged tRNA interacting with an appropriate codon in the A site. Then show two things that must happen before that tRNA can be found in the ribosome’s P-site.

20. Alcohol and caffeine increase urine volume by
A. decreasing blood pressure and increasing ADH production
B. increasing blood pressure and decreasing ADH production
C. increasing blood pressure and increasing ADH production
D. decreasing blood pressure and decreasing ADH production

Ubiquitination relies on 3 enzymes E1, which activate ubiquitin; E2, which are the conjugating enzymes, and E3 enzymes, which help guide the E2 enzymes to specific substrates. In any given cell there are just a couple types of E1 enzymes, several types of E2 enzymes that partner with small subsets of E3 enzymes, and even more types of E3 enzymes, which bind with small subsets of specific protein substrates. Given this information, which of these mutations would you hypothesize to be the most harmful to a cell?"

Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table. When the enzyme concentration is 3 nmol ml−1, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.00426 (μmol−1ml s).

a. Calculate Kcat for the reaction

b. Calculate Km for the enzyme

cWhen the reactions in part (B) are repeated in the presence of 12 μM of an uncompetitive inhibitor, the y-intercept of the Lineweaver-Burk plot is 0.352 (μmol−1 ml s). Calculate K′I for this inhibitor.

What is the FUNCTION (what it does, not what it is) of the following terms?

1.   Promoter –

2.   ORF –

3.   Compatible solute–

4.   Pasteurization –

5.   Bacteriostatic agent –

6.   Exposure of food to strong ionizing radiation–

7.   Fermentation of food –

8.   Glycolysis –

Identify all the metabolic processes we discussed that are anaerobic

How many molecules of ATP does a cell ultimately gain (not simply produce, but make in abundance of their own needs) when 4 molecules of glucose are broken down via glycolysis. Explain.

You are being strangled! Why do you die? Include in your answer a description of the need for O2 for each of the following processes and explain the specific consequences of O2 depletion on each.

-glycolysis

-the citric acid cycle

-the electron transport chain

-fermentation

1) Briefly explain what happens in the cellular respiration and what it function is
2) Briefly explain what happens in glycolysis and where it occurs ?
3) Describe what happens in the following processes and where they occur
a)glycogenesis
b)glycogenolysis
c)gluconeogenesis