If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate?

A.Increasing enzyme concentration has no effect on reaction rate.

1. List the 3 processes of Cellular Respiration in order.
A.
B.
C.

2. Where in the cell does each of the following occur?

Electron Transport:

Glycolysis:

Krebs Cycle:

3. Which process(es) requires Oxygen?


4. Which process(es) does not require Oxygen?


5. How much ATP is used for Glycolysis?


6. How much net ATP is made during Glycolysis?


7. Which of the 3 processes makes NADH?


8. Which of the 3 processes makes FADH2?



9. Which of the 3 processes makes CO2?



10. In no more than one sentence, what is the purpose of oxygen in cellular respiration?



11. What is the purpose of ATP Synthase?


12. What are the starting materials or reactants of Cellular Respiration?




13. What are the end materials or products of Cellular Respiration?




14. What is the product formed at the end of glycolysis?



15. What is the Net total of ATP made during Krebs Cycle?




16. What is the Net total of ATP made during Glycolysis?




17. What is the Net total of ATP made during Electron Transport?



18. What is the Net total of ATP made during the entire process of Cellular Respiration?



19. What are the super molecules NADH and FADH2 needed for?






20. Which of the 3 processes will NADH and FADH2 be utilized?

there are three bottles of unknown acids for analysis. One of the acids is sulfuric acid, H2SO4. one of the acids is nitric acid HNO3. one of the acids is hydrochloric acid, HCl. obtain a sample from each of the bottles and determine the identity of each acid. Describe the procedure you intend to use to perform this analysis

Summary

An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate, antibody-bound enzyme will cause a color change, thereby indicating the presence of the protein-of-interest in the sample.

In this video, the theory behind how ELISAs work is explained, including a discussion of both primary and secondary antibody binding and the importance of blocking steps. Theory is followed by practice, as the video progresses to an explanation of the step-by-step procedure. Finally, variations of the standard ELISA such as the sandwich and competitive ELISAs are introduced, and real world applications of this method, such as in over-the-counter pregnancy tests are explained.

1. For which of the following applications could an ELISA be used?

- To neutralize trypsin within a sample.

- To determine the size of a plasmid within a sample.

- To determine the presence or absence of contamination within a sample.

- To determine the presence or absence of a specific protein within a sample.

2. The target protein is recognized by...

- ...the substrate.

- ...buffer enzymes.

-...unlabeled viral particles.

-...the primary antibody.

3. The absorbance measured for each well is _____to the amount of target protein present in

each sample. (cell culture media harvested from human anti-body- producing cell lines).

- equal

- not directly related

- inversely proportional

- directly proportional

verify Stokes' thm.Assume that the surface S is oriented upward F = 2zi - 3xj + 4yk ; S that portion of the paraboloid z =16 - x²- y² for z>=0. My primary is how to convert dS into dA

evaluate the integral by making an appropriate change of variables

double integral of 5sin(25x^2+64y^2) dA, where R is the region in the first quadrant bounded by the ellipse 25x^2 +64y^2=1

11

Green Company sells its product for $11100 per unit. Variable costs per unit are: manufacturing, $5600; and selling and administrative, $125. Fixed costs are: $51000 manufacturing overhead, and $61000 selling and administrative. There was no beginning inventory at 1/1/18. Production was 34 units per year in 2018–2020. Sales were 34 units in 2018, 30 units in 2019, and 38 units in 2020. Income under absorption costing for 2020 is

Which enzyme contains the coenzyme biotin? What is the function of this coenzyme and how is it covalently attached to the enzyme? Show the steps in the mechanism of this enzyme.

What is glycolysis? What are the products of this process?

, apply the t-test to assess the following statement: "Men and women have different incomes in this city." Show your calculations and copy of the SPSS output in a Word document. In a separate 250-500 Word document, address the following questions: Describe what t-test is the most appropriate and explain why. Discuss whether you used a one-tailed or two-tailed test and explain why. Using SPSS, calculate the t-test and provide the test statistic and critical value assuming an alpha of .05. Calculate the effect size using r2. Interpret the results by (a) stating the reason the study or test was done, (b) presenting the main results, (c) explaining what the results mean, and (d) making suggestions for future research

.