1. Which of the following is the best description of a genome?

a. All of the genes of an organism

b. A sequence of DNA bases that code for a particular protein

c. A single molecule of DNA

d. A duplicated chromosome formed by DNA replication

2. The end result of mitosis is

a. two cells, each with half the number of chromosomes as the mother cell.

b. two cells, each with twice the number of chromosomes as the mother cell.

c. two cells identical in genetic material, but one is smaller than the other.

d. two cells identical in size and genetic material.

3. In an adult human, where would you find active mitosis occurring?

a. Bone marrow

b. Brain

c. Muscles

d. Testes and ovaries

4. In an adult human, where would you find active meiosis occurring?

a. Muscles

b. Brain

c. Bone marrow

d. Testes and ovaries

5. If meiosis occurs in a cell with a diploid number of chromosomes of 16 how many chromosomes will be in each daughter cell?

a. 4

b. 16

c. 8

d. 2

1. The experiments that clearly showed that DNA from every organism studied had an amount of adenine equal to the amount of thymine and a cytosine content equal to the content of guanine were conducted by:

a.   E. M. Sutton.

b.   Cohen and Boyer.

c.   Hershey and Chase.

d.   Erwin Chargaff.

e.   Rosalind Franklin.

2. A quiescent (non-dividing) human cell is most likely (or most correctly described as being) in:
   1. G1.
   2. S phase.
   3. G0
   4. M phase.
   5. cytokinesis.

3. Who utilized the process of transformation, as originally demonstrated by Frederick Griffith, to provide the first solid evidence suggesting that DNA was the genetic material of living systems (in this case bacteria)?
   1. Messelson and Stahl
   2. Thomas Morgan
   3. Erwin Chargaff
   4. Hershey and Chase
   5. Avery, McCarty and Macleod

4. The two strands of a DNA helix are initially separated during the process of DNA replication by:

1. DNA polymerase I.
2. DNA polymerase III.
3. a topoisomerase.
4. primase.
5. a helicas

5. To avoid problems associated with gene dosage issues (noting that males are XY), human XX females convert one X chromosome into a/an:
   1. Barr body.
   2. centromere.
   3. centrosome.
   4. telomere.
   5. balanced translocation.

Find the human hemoglobin subunit alpha protein on PDB (Protein Data Bank) protein database. Hint: You can use also use PDB identifier “5HU6” to find human hemoglobin subunit alpha protein on PDB database.

(a) Find the Experimental Data section on this protein page and report Method and Resolution used?

(b) Find the Entry History section on this protein page and report Deposited Date and Deposition author(s)?

(c) Find the secondary structure information for this protein structure. What secondary structure types does this protein contain?

(d) How many helices are there? How many beta strands are there?

(e) Download the FASTA Sequence and Copy/Paste the sequence information from the downloaded file

(f) Click 3D View tab and view the protein structure in 3D. Then, take a screenshot of the Protein structure on the viewer and provide the screenshot

(g) Click the Sequence Similarity tab and write down how many sequences on PDB database are 70% or more similar to hemoglobin subunit alpha( it is the number is shown under “Chains in Cluster”)

You are a researcher at a small biotech company and your company has just obtained the license for use of a human GENOMIC DNA fragment putatively encoding a potentially novel protein, which is thought to regulate p53, the known tumor supressor protein. The scientists who originally cloned this GENE fragment HDM5 "claim" that HDM5 shares 90% DNA sequence homology with one of the HDM2 genes (refer to the review Levine & Oren, 2009). They propose that HDM5 may have HDM2-like properties and may be involved in regulating cell proliferation, and thus a good target to potentially develop as a cancer therapy. Your company has asked you to characterize the gene and gene products, as well as to provide an opinion as to its potential human therapeutic uses.

1. Before proceeding, you wish to know whether this gene is actually expressed in humans (your target market for therapeutic development). ?Describe, in detail, 2 efficient experimental approaches to answer this question. ?Discuss whether your proposed methods measure synthesis or accumulation of the product

Week 10 Assignment - Fiscal Planning and Management Presentation

Read and use this for this part of the assignment and do as directed please.

You are a regional healthcare administrator for a long-term care organization. You have been asked to provide training to a new group of healthcare administrators, who just completed their degree. They have no work experience in healthcare administration or financial management.

For this assignment, you are to:

1. Develop a presentation using PowerPoint slides that explain the role of healthcare administrators in fiscal planning and financial management.

Be sure to address the following components:

1. Strategic planning.

2. Budgeting.

3. Regulatory compliance.

4. Insurance and reimbursement.

5. Human resources/human capital/staffing.

2. Develop a 10–12 slide presentation with detailed speaker notes. (Your cover slide and references slide are not counted in the the 10-12 slides they are separate).

Go to Google Scholar Databases to find and include 3–5 quality references, one must be your course textbook.

Also, list references used please so they can be put on a slide.

Write in C++

Abstract/Virtual Rock Paper Scissors

Create an abstract Player class that consists of private data for name, selection, wins, and losses. It must have a non-default constructor that requires name. It may not contain a default constructor. Create overloaded functions for the ++ and - - operator. The overloaded ++operator will add to the number of wins, while the - - operator will add to the losses.

You will create two different child classes of player, Human and Computer. Neither of which will have any private data, as they will use the parent’s data. Both will contain a virtual function called makeSelection() that will determine and set the selection for the particular player.

Your main() program will contain a NON-member function called playGame() that will take in your two players. You should ask the user if they would like to pay against another human, or a computer, or if they would like to see two computers play.

You will then need to create the appropriate objects and start the game play. After each round, you must display the number of wins and losses for each player and continue playing the game until the user decides to quit.

The presentation slides

This assignment requires you to look at one interface for a product of your choice, identify what it was developed for, and then trace back its history (to the origin if possible) to look for similar solutions. This way you'll be clearer what the original problem is and how human through out history has managed it. You'll see that whole or part of the very early solutions seem to stay in most of the newer solutions, and you'll be able to identify and explain which solution are more effective than others; and you'll probably can predict how the next generation of solution would be.

For example, if you look at the phone today and some of the phones have been in the market in the last 50 years, You'll see there are some common functionality, interface, and user's interactions. If you keep tracing back, you'll know why telephone was invented and the reason why it works in a certain way. Ask yourself, what human had used to accomplish similar tasks the phone provides them before it was invented. You'll get the entire picture.

Researchers now believe that most medical errors cannot be prevented by perfecting the technical work of individual doctors, nurses, or pharmacists. Improving patient safety often involves the coordinated efforts of multiple members of the health care team, who may adopt strategies from outside health care.”

“The report reviews several practices whose evidence came from the domains of commercial aviation, nuclear safety, and aerospace, and the disciplines of human factors, engineering and organizational theory. Such practices include root cause analysis, computerized physician order entry and decision support, auto-mated medication dispensing systems, bar coding technology, aviation-style preoperative checklists, promoting a ‘culture of safety,’ crew resource management, the use of simulators in training, and integrating human factors theory into the design of medical devices and alarms.

” Discuss this concept of utilizing standard business quality initiatives and the logic of adopting them to use in healthcare. Using the practice of Root cause analysis, research, summarize, and include how you might apply this concept in your healthcare facility

Your colleague in the lab next door is studying the disease cerebral cavernous malformations (CCM) and discovers that the human homologue of the worm exc12 gene (human gene is HEXC12) is mutated in a small fraction of familial CCM patients. You decide to collaborate to test if this gene has a CCM phenotype in mouse, cultured cells, and zebrafish models.

1. Explain how you would generate a mouse model.

2. Based on its sequence you hypothesize that HEXC12 encodes as a small GTPase exchange protein (GEF) that might regulate the activity of RhoA or CDC42. Explain how you would test this hypothesis using cultured cells.

3. How would you determine if HEXC12 regulates the activity of the Rho kinase (ROCK) using cultured mammalian cells?

4. How would you determine if HEXC12 is required in the endothelium or surrounding smooth muscle for proper vascular development in the mouse?

5. To gain more insight into HEXC12 you decide to use zebrafish as a model system. Explain how you would determine if it has a CCM phenotype in fish.

Identify two important operating standards that are routinely measured in your organization. These measures must meet the criteria for qualitative, nominal measures. Observations must be cross-classified into a contingency table. Obtain a sample that will produce at least five observations into each group of the cross-classification.

Purpose:  To demonstrate the application of non-parametric tests in your organization.

Example:  A standard measure that fits these requirements is the human resource department’s employee tracking system. The director of human resources of my company wants to do a study to determine if there is a relationship between the management status and gender of the employees. She creates a contingency table that has Male or Female categories for the rows, and Manager or non-manager categories for the columns. Then she counts the number of female managers, female non-managers, male managers, and male non-managers and enters the numbers in the table. This is the observed value table. Then she conducts the hypothesis test.

a)      Perform the cross-classification of the two nominal variables into a contingency table.

b)       Test the hypothesis that there is no relationship between the two variables.

c)       Discuss the result and interpretation of your hypothesis test