The following is a gene sequences in human that related to ovarian cancer. Design a pair of primers for PCR amplification of this gene using Primer 3. Print the primer 3 output, and answer the following questions:

DNA sequences

•1 ttaaaagttc actggaactg gaagaaatgg atttatctgc cgtccaaatt caagaagtac 61 aaaatgtcct tcatgctatg cagaaaatct tagagtgtcc gatctgtttg gaactgatca 121 aagaacctgt ttccacaaag tgtgaccaca tattttgcaa attttgtatg ctgaaacttc 181 ttaaccagaa gaaagggcct tcacaatgtc ctttgtgtaa gaatgagata accaaaagga 241 gcctacaggg aagcacaagg tttagtcagc ttgctgaaga gctgctgaga ataatggctg 301 cttttgagct tgacacggga atgcagctta caaatggttt tagtttttca aaaaagagaa 361 ataattcttg tgagcgtttg aatgaggagg cgtcgatcat ccagagcgtg ggctaccgga 421 accgtgtcag aaggcttccc caggtcgaac ctggaaatgc caccttgaag gacagcctag 481 gtgtccagct gtctaacctt ggaatcgtga gatcagtgaa gaaaaacagg cagacccaac 541 ctcgaaagaa atctgtctac attgaactag actctgattc ttctgaagag acagtaacta 601 agccaggtga ttgcagtgtg agagaccagg aattgttaca gaccgcccct caagaagctg 661 gagatgaagg caagctgcac tctgcagaag aggctgcttg tgagttttct gagggcataa 721 gaaacattga acatcatcaa tgcagtgatg atttaaaccc tactgagaat catgcaactg 781 aaaggcatcc agaaaaatgt cagagtattt ctatttcaaa tgtgtgtgtg gagccatgtg 841 gcacagatgc tcatgccagc tcattacagc ctgagaccag cagtttattg ctcattgaag 901 acagaatgaa tgcagaaaag gctgaattct gtaataaaag caaacagcct ggcatagcag 961 tgagccagca gagcagatgg gctgcaagta aaggaacatg taacgacagg caggttccca 1021 gcactgggga aaaggtaggt ccaaacgctg actcccttag tgatagagag aagtggactc 1081 acccgcaaag tctgtgccct gagaattctg gagctaccac cgatgttcct tggataacac 1141 taaatagcag cgttcagaaa gttaatgagt ggttttccag aactggtgaa atgttaactt 1201 ctgacagcgc atctgccagg aggcacgagt caaatgctga agcagctgtt gtgttggaag 1261 tttcaaacga agtggatggg ggttttagtt cttcaaggaa aacagactta gtaacccccg 1321 acccccatca tactttaatg tgtaaaagtg gaagagactt ctccaaacca gtagaggata 1381 atatcagtga taaaatattt gggaaatcct atcagagaaa gggaagccgc cctcacctga 1441 accatgtgac tgaaattata ggcacattta ttacagaacc acagataaca caagagcagc 1501 ccttcacaaa taaattaaaa cgtaagagaa gtacatccct tcaacctgag gacttcatca 1561 agaaagcaga ttcagcaggt gttcaaagga ctcctgacaa cataaatcag ggaactgacc 1621 taatggagcc aaatgagcaa gcagtgagta ctaccagtaa ctgtcaggag aacaaaatag

1. What is the sequences of the primers? (5’-3’)

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2.What is the size of amplification products

3.What is the length of your primer?

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4.What is your GC content of your primer (GC%)?

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Assume that the duration of human pregnancies can be described by a Normal model with mean 268 days and standard deviation 14 days.

​a) What percentage of pregnancies should last between 274 and 281 days? (Round to two decimal places as needed.)

​b) At least how many days should the longest 25​% of all pregnancies​ last? (Round to one decimal place as needed.)

​c) Suppose a certain obstetrician is currently providing prenatal care to 58 pregnant women. Let y overbar represent the mean length of their pregnancies. According to the Central Limit​ Theorem, what's the distribution of this sample​ mean, y overbar​? Specify the​ model, mean, and standard deviation.

A. A normal model with mean ___ and standard deviation ___. (Type integers or decimals rounded to two decimal places as needed.)

B. A binomial model with ___ trials and a probability of success of ___. (Type integers or decimals rounded to two decimal places as needed.)

C. There is no model that fits this distribution.

​d) What's the probability that the mean duration of these​ patients' pregnancies will be less than

258 days?

- The probability that the mean duration of these patients' pregnancies will be less than 258 days is ___. (Round to four decimal places as needed.)

​days?

1. At the peak of an action potential, the membrane potential of a human motor neuron reaches about +30mV. What value for “alpha”, the ratio of PNa/PK, allows this to occur. Use an online calculator/simulation to find a value for alpha that approximates what happens to reach this membrane potential, and write out the entire GHK equation (the version with just sodium and potassium) that can produce this membrane potential, including normal ion concentrations and temperature.

The human resources department of a very large organisation is trying to determine the proportion of all employees that are satisfied with their current position. They randomly select 121 employees and ask them: "Are you satisfied with your current position?" 61 replied yes they were. Construct a 95% confidence interval to estimate the true proportion of all employees at this workplace who were satisfied with their position. A 95% confidence interval for the true proportion of all employees at this workplace who were satisfied with their position is between (round your answers to 2 dp) Answer and Answer.

A study is planned on the physiology of exercises with human subject volunteers. The two treatments in the study are two methods of aerobic exercise training (call the methods A and B). At the end of a ten-week exercise period, each subject will undergo a treadmill test for standard respiratory and cardiovascular measurements.

Nineteen volunteers are listed in the table by sex and age. All volunteers are in good health and in the normal weight range for their age, sex, and height. Eight individuals will be tested in each the methods (A or B), so that only 16 of the 19 volunteers will be used; a subject will participate only in one of the methods.

a. Explain how you would group the individuals prior assignment of treatments so that experimental error variance could be kept at a minimum. b. Explain why you grouped as you did. c. Show your final assignment of individuals to the treatment groups.

Primatologists are interested in the topic of altruism in order to determine where the human behavior arises. Altruism is defined as actions that benefit another individual but at some potential risk or cost to oneself. The act of ‘doing a good deed’ for others in humans counts as altruism, because there is some level of sacrifice in effort or resources from the individual that helps the other. Scientists argue that there are ulterior motives on the part of primates that they study. The good deed will be returned at a later date. We can test the importance of altruism among humans with this small science project.

Goal: Assess the degree of altruism among humans. Does it is exist? Method: Groom someone or do something altruistic to help someone who you will see in the near future for several days. Then assess whether they feel compelled to return the favor. For example, you may actually groom someone by pretending to pick something off of their clothes or buying them coffee. Then put them in the same but opposite situation later on. You could leave something on your clothes to see if they pick it off. You can try other forms of social grooming such as complementing someone as well. Will they return the favor later on? Try to devise new and original ways to test this idea of altruism through grooming.

Write up: Does this act bring you closer? What does it say about the grooming among humans? Is this evidence that it was selected for as a behavioral trait? Also try to explain WHY it came about as a behavioral trait (or WHY it didn’t if the data comes out negative).

9. As part of a study of corporate employees, the director of human resources for PNC Inc. wants to compare the distance traveled to work by employees at its office in downtown Cincinnati with the distance for those in downtown Pittsburgh. A sample of 35 Cincinnati employees showed they travel a mean of 370 miles per month. A sample of 40 Pittsburgh employees showed they travel a mean of 380 miles per month. The population standard deviations for the Cincinnati and Pittsburgh employees are 30 and 26 miles, respectively. By following the six-step procedure for hypothesis testing found below, answer the following: At the 0.05 significance level, is there a difference in the mean number of miles traveled per month between Cincinnati and Pittsburgh employees?

Step 1: State the Null Hypothesis (H_0) and the Alternate Hypothesis (H_1)

Step 2: Determine the level of significance. (Note: It’s given in this problem!)

Step 3: Select the Test Statistic

Step 4: Formulate the Decision Rule

Step 5: Make a Decision

Step 6: Interpret the Result

When the human body is invaded by foreign microorganisms such as bacteria and viruses, it produces an immune response. Choose one example of a bacterium or virus to discuss.

• What actions does the body produce in its immune response to invasion by the particular bacterium or virus you selected? Explain your answer.
• Would active or passive immunity be more effective against this bacterium or virus? Why?

QUESTION 1

1. A major theme in human anatomy and physiology is the complementarity of structure and function, meaning that the structure of an organ, for example, will reflect that organ’s ability to function optimally, and vice versa.  Please discuss in detail how the structure of the cell membrane and its unique chemistry are the factors that determine the selectivity of the membrane.  Be specific and be detailed.  A 2 or 3 sentence response will not be considered an adequate response.

You will then find one human to interview. This can be anyone you know or a stranger.

The interview should be conducted in-person or through skype or FaceTime.

The interview should be informal and should focus on any information that you want to learn about your subject. More will be discussed in class about generating interview questions.

Try to think of roughly 10 questions to ask your subject. Focus on aspects of the subject’s life, career, family, future aspirations etc.…

You do not need to record the interview but may take notes.

During the interview you want to pay attention to your subject’s behavior, word choice, and nonverbal messages.

You will then compile all your information and compose an essay that:

Discusses your interview experience from an interpersonal perspective.

You may discuss things such as Self-disclosure, equivocation, conflict, verbal, non-verbal messages etc…

The idea is to give me a sense of how you felt before, during, and after the interview.

Were there any verbal or non-verbal messages that confused you or made you feel relaxed?

Did you or the interview subject use self-disclosure and if so how did it help or hurt the experience?

You may discuss any term, theory, or concept that relates to interpersonal communication.

You do not need to provide direct quotes from the interview or any specific content from the experience. Instead you want to focus the essay on discussing your experience through an interpersonal perspective. Try to work-in/discuss as many terms and concepts from your textbook that relate to interpersonal communication as possible.