. You want to improve your protein to have desired properties by introducing random mutations only into the selected spot (hatched in the below diagram), but not whole regions, of the protein. What kind of mutation technique will you use to generate protein library to introduce random mutations to only the selected regions? Please explain your chosen mutation method in detail, including your primer design and PCR strategy

Write in detail about the effects of SAD on international students in Canada. Google and read articles on the following topics and use them in your essay: -Effects of SAD on mental health in students, Effects of SAD on physical health, Effects on work and studies and Effects on relationships.

Question explain in detail:

Explain alternation of generations in the life cycle of an organism. Use an example from the plant kingdom and discuss how that group of plants (Gymnosperm, Angiosperm, etc.) accomplishes reproduction by alternation of generations.

Explain in detail the experimental procedure you would use for determining the concentration of acetic acid in an aged wine sample. ( Answer in detail)

Explain cell division in detail. You may use images or figures if you wish. 25 scores
2. Integrate the knowledge provided you about the regulation of blood pressure in different topics including the physiology of Endocrine system, Renal & Fluid, Nervous system, and Cardiovascular system. Try to integrate all of this data and draw a scheme/trace (like the figure below) to explain the mechanism involved in regulation of blood pressure. 25 scores

..... ......
.... .....
BP .... ........

Charlie has just learned about web services architecture and is convinced that it is the best thing since sliced bread! Even better, he has just found out that Amazon offers a set of its web services for developers to use. He just can't wait to tell Suzanne about this.

Visit the website provided in the Optional Resources section of the Unit Schedule or find a similar web services provider to learn about web services that are provided to developers.

Then, answer the following questions.

• Explain why you would or would not consider developing an application based on these web services.
• Do you agree with Charlie that web services architecture is an amazing technology and should definitely be used in the PPI project? Justify your response.

Instructions:  Save this file as YourName_PracticeDebuggingTest.

- Part 1 – 4 Debugging Exercises – there are things wrong – either logic or syntax errors.  Correct the pseudocode and one Java program. Add the corrected code in BOLD and change the corrected color to RED.  

Part 1

Debugging 1:  Add the missing code in bold and red.

start

         Declarations

                                    num firstNum

                                    num secondNum

                                    string MSG = “Got it!”

                  housekeeping()

                  detail()

                  finish()

         stop

         housekeeping()

                  output “Enter three numbers: ”

                  input firstNum, thirdNum

         return

         detail()

                  if ((firstNm + secondNum) = thirdnum) OR

                     ((secondNum + thirdNum) = firstNum)) OR

                     ((firstNum + thirdNum) = secondNum) then

                                    output MSNG

                  endif                          

return

finish()

         output “End of program”

return

Describe in detail as great a detail as possible the mechanism by which skeletal muscle cells contract. Start with

an action potential arriving at an axon terminal of a motor neuron and end with the completion of a single cross-

bridge cycle. Be sure you describe all the events and how the operate, and name all the structures, ions, proteins,

molecules involved and explain the role each has. Use proper terminology and explain what is happening at

each step. Then explain what has to occur in order for a muscle cell to relax. This will require a full page.

Mr. Tom has a small distillery with two employees. He currently brews a moonshines which is uncage corn whisky and apple pie liquor which combines his moonshine and apple cider from murphy`s orchard. Describe how the business can “operate.” If they make a product, describe the production. If they offer a service, describe each step of that service. If they are a retailer, location, product mix, and suppliers are important. Think through your business' daily operation and explain it in detail. Then, think about who, what, and how each of those steps will happen. Realistically, how much of this can one person do? Strategically, how will their plan for growth? Please be detail