Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment, plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate).

            5) What does LB (-) plate contain?

6) What does LB/ amp (-) plate contain?

7) What does LB/amp (+) plate contain?

8) What does LB/amp/ara (+) plate contain?

A student performs this experiment but does not have the flask boiling when dissolving the original sample. All other steps are performed correctly (using the minimum amount of solvent to dissolve as much of the sample as possible, etc.). What will be the effect on this student’s eventual percent recovery after recrystallization as a result of this mistake? Explain in one sentence.

A student performs this experiment but forgets to rinse the product after vacuum filtration. Which type(s) of impurity would likely be present the following week? Choose among: “soluble”, “insoluble”, “both”, or “neither”, and explain in one sentence.

1)When 5.352 grams of a hydrocarbon, C_xH_y, were burned in a combustion analysis apparatus, 18.38 grams of CO₂ and 3.010grams of H₂O were produced.
In a separate experiment, the molar mass of the compound was found to be 128.2 g/mol. Determine the empirical formula and the molecular formula of the hydrocarbon.

2)When 4.948 grams of a hydrocarbon, C_xH_y, were burned in a combustion analysis apparatus, 16.72 grams of CO₂ and 3.424grams of H₂O were produced.
In a separate experiment, the molar mass of the compound was found to be 26.04 g/mol. Determine the empirical formula and the molecular formula of the hydrocarbon.

Chemistry Calorimetry experiment questions
1. Why is dissolving of ammonium chloride endothermic? would you get similar results for dissolving all salts? explain your answer using another salt as an example.
2. Suppose the calorimeter used for this experiment had been made of a heat-conducting material (such as metal) rather than styrofoam. Would the measured calorimeter constant be larger or smaller. Explain your answer.
3. Why do you use the heat capacity of 2.5M NaCl solution rather than the heat capacities of the original acid and base solutions?

Consider an experiment with four independent variables: A, B, C, and D.

Factor A has 3 levels and is a between-subjects variable; Factor B has 2 levels and is a within-subjects variable; Factor C has 2 levels and is a between-subjects variable; Factor D has 3 levels and is a within-subjects variable.

1. How many “cells” or “conditions” or “groups” are there in this experiment?

2. If you want to test 10 participants per cell, how many TOTAL participants will you need?

3. Identify ALL of the effects to be tested via ANOVA

In a chemistry experiment , 2ml of 5% sodium hypochlorite, 2ml of 3M KBr and 2ml of 3M KI are individually put to a 150 ml test tube(separately). then 10 ml of cyclohexane is added to all three tes tubes and shaked. then after that the 6M hcl, 8M Hno3, 8M Hno3 are respectively added to the 1st, 2nd and third solutions. After that the test tubes are pit on a hot water bath to increase the reactions for the second and third solitions. Explain thoroughly about the use of the experiment ,all the color changes, the layer formed, everything?

In this experiment, you will have to prepare your dosing solutions. Look at the plate layout from your experiment and assume that you need 100 µL of dosing solution per well when dosing 16 wells (this already includes ample surplus) per concentration. Calculate how much MeOH and media is required for each solution.


0% MeOH




1% MeOH

5% MeOH




10% MeOH




20% MeOH




40% MeOH




70% MeOH (double the volume, since you use this dosing solution in your dead wells and your negative control)

1. Earlier research has implicated the BMP4 gene as being important in the shape of birds’ beaks. A Genome Wide Association Study of beak shape in finches from the Galapagos Islands identified a number of loci that contribute to beak shape in finch species but BMP4 was not among the candidate genes identified in that study.
   1. Speculate about why BMP4 was not found in the GWAS experiment.

2. As the genome sequence for the finches studied in the GWAS experiment is now publicly available, how could you use the sequence to determine whether or not BMP4 is involved in beak shape differences in finches?