1. What sorts of residues (i.e. what amino acid side chains) would one expect to find on the surface of a protein with an unusually high isoelectric point (e.g. 9.5). What if the pI were unusually low (e.g. 3.4)?
2. When one talks about “protein-ligand” interactions, what sorts of molecules can be “ligands?”
3. How would you explain the conformational selection model of protein-ligand interactions to an educated lay person?
In: Chemistry
Both myoglobin and hemoglobin exhibit cooperative binding to oxygen
true or false
Tropocollagen is a double helix of two left handed polypeptide chains
true or false
F actin is a polymer of G actin monomers and exhibits symmetry
true or false
Proteins have an asymmetrical tertiary structure, while multisubunit proteins can exhibit several types of symmetry
true or false
When referring to the amino acid sequences of proteins, sequence homology is the same as sequence similarity.
true or false
In: Chemistry
a. Identify hydrogen bond donors and acceptors in each Watson-Crick base pair. Why won't adenine base hydrogen bond with cytosine or guanine hydrogen bond with thymine?
b. Name an important structure stabilized by Van der Waals interactions. Explain how the Van der Waals interactions work in this instance
c. Explain how the ionization state of any amino acid (or its side chain in a protein) at any
pH can be predicted, based on its pKa value.
In: Biology
In humans, the huntingtin gene is essential for nerve cells to function effectively. People with Huntington’s disease have 2 different copies of the huntingtin gene – one normal and one mutated. The mutated huntingtin gene (HD) is caused by an excess of codons (CAG) that code for the amino acid glutamine. The HD gene is responsible for deterioration of the nerve cells, which leads to loss of one’s ability to move, feel and think.
Can you design an RNAi drug that targets only the HD gene and not the normal huntingtin gene?
In: Biology
The following is the nucleotide sequence of a strand of DNA from E. coli. CGTCCTCCAATCGCCCGTACCGTCTCCAGCGGAGATCTTTTCCGGTCGCAACTGAGGTTGATCAAC
The strand is transcribed from left to right and codes for a small peptide.
a) Is this the mRNA-like coding strand or template strand?
b) Which end is the 3' end and which is the 5' end?
c) What is the DNA coding strand sequence of the ORF ?
d) What is the sequence of the entire transcript (assume the +1 of transcription begins at the first nucleotide shown)?
e) What is the amino acid sequence of the peptide?
In: Biology
1.The purpose of the indole test is to differentiate groups of gram-negative, lactose-fermenting bacteria called___________ . This specific test allows to identify bacteria that are capable of hydrolyzing the amino acid___________ . This is achieved by detecting the presence of the enzyme___________
2.Which of the following products are made from this hydrolysis reaction? (select all)
You can select more than one option
A.Indole
B.Oxygen
C.Ammonia
D.Pyruvate
E. Glucose
F.Carbon Dixoide
G. Citrate
Glucose
F
Carbon dioxide
In: Biology