The enzyme is studied in the presence of a different inhibitor (inhibitor B). In this case, two different concentrations of inhibitor are used. Data are as follows:
a) Determine the apparent Vmax at each inhibitor concentration.
b) Determine the apparent KM at each inhibitor concentration.
c) Estimate KI from these data. [Hint: Calculate the KI at 3 mM and at 5 mM; and then take the average of the two KI values.]
| v[(mmol/L)min−1] | |||
| [S](mmol/L) | No inhibitor | 3 mM inhibitor B | 5 mM inhibitor B |
| 1.25 | 1.72 | 1.25 | 1.01 |
| 1.67 | 2.04 | 1.54 | 1.26 |
| 2.50 | 2.63 | 2.00 | 1.72 |
| 5.00 | 3.33 | 2.86 | 2.56 |
| 10.00 | 4.17 | 3.70 | 3.49 |
In: Chemistry
Urea was hydrolyzed with an enzyme at a fixed concentration of 50 μM and the following data were obtained. Using Lineweaver-Burk plot determine the kinetic parameters.
|
Urea(mM) |
0.10 |
0.20 |
0.30 |
0.40 |
0.50 |
0.60 |
0.7 |
0.80 |
0.90 |
1.00 |
|
Rate(mMs-1) |
6.25 |
12.5 |
18.8 |
25.0 |
31.2 |
37.5 |
43.7 |
50.0 |
56.2 |
62.5 |
In: Other
Which of the following mechanisms by which a human retrovirus causes cancer in humans?
the retro virus causes over expression of the human photo - oncogene
In: Biology
Explain why it is generally easier to control (with antimicrobial chemicals) a bacterial pathogen in the human body than it is to control a fungal pathogen in the human body.
In: Biology
how one large company implemented a workforce planning and design process to move to a human capital perspective on strategic human resource management.?
In: Economics
What are the similarities and differences between the acquisition of language in human infants and the evolution of language in the human species? Answer this question in terms of Tomasello’s theory.
In: Biology
From the perspective of other animals, human sexuality seems "abnormal". In what ways are human sexualities similar to and different from, other animals?
In: Psychology
What is the scope of Human Resource Planning (HRP) Exercise?
Benefit of Workforce Planning and effective HRP?
Evolution of HRP Human Resource Planning?
In: Operations Management
Human Resource Strategy
Discuss human resource strategies that support a Cost Leadership competitive Strategy. Provide examples to illustrate your answer.
In: Accounting
1. Which of the following is not an example of a posttranslational modification?
A. N-terminal amino acid(s) commonly removed or modified
B. signal/targeting peptide remova
l C. phosphorylation, methylation, acetylation
D. Glycosylation
E. All of the above are examples of posttranslational modifications
2. The major problem that arises from incorporating incorrect tautomers into a cell’s DNA is that:
A. they react with adjacent bases to form dimers.
B. they often lead to slippage problems during replication.
C. they cannot be transcribed into RNA, blocking gene expression.
D. they mispair, leading to replication errors.
E. All of the above are correct answers to the question.
3.An alkylating agent reacts with the double-bonded oxygen of a guanine base in a DNA molecule. The most likely result will be:
A. the base is locked into the incorrect tautomer.
B. that it will dimerize.
C. that it will fall out of the duplex as the n-glycosidic bond fails.
D. a strand break as the phosphodiester bond will fail.
E. a deletion in the DNA at this point.
4. The treatment of eukaryotic cells with 5-azacytidine is likely to:
A. cause a general reduction in gene expression.
B. activate a number of previously inactive genes.
C. cause mutations of the transition variety.
D. cause a shortening of telomeres.
E. block cell division in S phase.
5. In the context of eukaryotic gene expression, which of the following statements about enhancers is/are not true?
A. They can be upstream or downstream of the regulated promoter(s).
B. They can act over relatively large distances.
C. The element’s orientation is not important for function.
D. They are gene specific.
E. They may have binding sites for both activating and inhibitory factors
6.A study of one of your cells reveals that it has many DMs and HSRs. This suggests that it has:
A. a high level of heterochromatin.
B. an unstable genome.
C. engaged in extensive chromatin remodeling.
D. heavily damaged DNA.
E. entered into S phase and is replicating its DNA.
7.A protein thought to be involved in the process of activating a eukaryotic gene though the process of chromatin remodeling is:
A. HDAC.
B. HAT.
C. CAP.
D. photolyase.
E. CpG DNA methylase
8.. During the elongation phase of translation, the charged tRNA enters the ribosome at the: “
A. A” site.
B. AUG codon.
C. “E” site.
D. “P” site.
E.RBS.
9. A molecule with a planar conjugated ring structure interacts with an E. coli cell’s DNA in the “early” portion of a coding sequence for an essential enzyme and eventually leads to a mutation in this region. The likely result will be:
A. nonsense mutation.
B.missense mutation.
C. a silent mutation.
D. frameshift mutation.
E. conditional mutation.
In: Biology