You are doing an EMSA experiment. The DNA sequence you study is about 1000bp long and contains the first two exons of a protein coding gene. You run your DNA alone on the gel for 1 hour at 72 volts and you notice that the DNA fragment matches the 1000bp marker in the DNA ladder, which is to be expected. You then start adding different transcription factors to your DNA sample and run the EMSA experiment to see which one would bind to your DNA. After adding 10 different transcription factor proteins to your DNA sample and doing the EMSA experiment you see that none of them changes the mobility of you DNA fragment. Explain this observation.

1. When we experiment, we always want to think about sources of experimental uncertainty. In the lab "Measurement and Uncertainty" you learned about random errors and systematic errors. Let's think about some potential sources of uncertainty in this experiment.

   If the uncertainty is random, fill in R. If it is systematic, fill in S.

   In every trial, a student holds the picket fence so that it is tilted slightly to the left as it drops through the photogate.

   In the course of the experiment, the student holds the picket fence pretty close to vertical, but some times there is a slight tilt to either the left or right.

   One student group cannot find their picket fence. The decide to just drop a tennis ball through the photogate instead.

As we have seen in class, hypothesis testing and confidence intervals are the most common inferential tools used in statistics. Imagine that you have been tasked with designing an experiment to determine reliably if a patient should be diagnosed with diabetes based on their blood test results. Create a short outline of your experiment, including all of the following:

1. A detailed discussion of your experimental design.
2. How is randomization used in your sampling or assignment strategy?
3. The type of inferential test utilized in your experiment.
4. A formal statement of the null and alternative hypothesis for your test.
5. A confidence interval for estimating the parameter in your test.
6. An interpretation of your p-value and confidence interval, including what they mean in context of your experimental design

Using JavaScript

You must submit a file called, called pass.js

pass.js: Validator of passwords

Your task this week will be to program a valid password validator.

A password is safe only if it satisfies the following constraints:

- It has from 5 to 10 characters (inclusive)
- It contains at least one lowercase letter, at least one capital letter and at least one number
- The first character is different from the last

Once the password is entered and validated, the user is asked to copy it again to validate.

Course of the program

More precisely :

1. Ask the user to enter a password with:

      prompt ("Please choose a password");

2. Check if the password matches the requirements

   2a. If the password is invalid, display "Invalid password" and end the program (the rest of the program runs only if this step was done correctly)

   2b. If the password is correct, go to step 3

3. Ask the user to enter the password again with:

      prompt ("Please enter the password a second time to confirm");

4. Check if both passwords are the same

   4a. If the passwords do not match, show "You did not enter the same password twice" and ask to start again
   4b. If the two entered passwords match, go to step 5

5. Once the password is valid and entered twice correctly, display "Password saved!"

Here is an example of the execution of the program :

Please choose a password
> the user enters "abc"
Invalid password

Another example :

Please choose a password
> the user enters "l33th4xx0r"
Please enter the password a second time to confirm
> the user enters "abc"
You have not entered the same password twice
Please enter the password a second time to confirm
> the user enters "l33th4xx0r"
Password saved!

Your program must be complete, with comments that
indicate the name of the file, the author (your name), a brief
description of the usefulness of the program. There must also be
comments that explain what each variable corresponds to,
and the operation of the program. Use statements of
appropriate loop, correct indentation, and block statements
in the body of if and loops. Your program should avoid
redundant and repetitive calculations.

The President P has been accused of colluding with Russian intelligence to tilt the election in his favor. Let g denote “how guilty” the President is of these charges. Assume g is either 1, 2, or 3, where 3 means “guilty as sin” and 1 means “not at all guilty.” Assume that g is chosen by nature and a voter V believes each value is equally likely (voters are pretty skeptical of politicians these days). A special counsel S has been appointed to investigate the accusation. If S is allowed to complete her work, she will learn the value of g and report it to the voter V . However, P might fire S before she is allowed to complete her work, in which case S cannot report anything to V . Assume that nature draws g, shows it to P, and then P decides whether to fire Ior not. If so, the “game” ends; if not, S reports g to V , and the game ends. Assume P ’s utility is ?1 times V ’s expectation of g at the end of the game (i.e., P is better off, the lower is the voter’s assessment of his guilt).

(c) Suppose all types except g = 1 fire S. What does V think is the expected value of g, given that S is fired? Is type g = 2 happy with that?

(d) Is it a perfect Bayesian equilibrium for all types to fire S? For type g = 1 not to fire S, and for the other types to fire S?

(e) Assuming P is rational, what should V conclude about g, if S is fired?

At December 31, 2015, certain accounts included in the property, plant, and equipment section of Kevin Company’s balance sheet had the following balances:

Land          ........................................................................... $200,000

Buildings    ........................................................................... $900,000

Leasehold improvements..................................................... $600,000

Machinery and equipment.................................................. $700,000

During 2016, the following transactions occurred:

Land site number 621 was acquired for $1,000,000. Additionally, to acquire the land, Kevin paid a $60,000 commission to a real estate agent. Costs of $15,000 were incurred to clear the land. During the course of clearing the land, timber and gravel were recovered and sold for $5,000.

A second tract of land (site number 622) with a building was acquired for $300,000. The closing statement indicated that the land value was $200,000 and the building value was $100,000. Shortly after acquisition, the building was demolished at a cost of $30,000. A new building was constructed for $150,000 plus the following costs:

Excavation fees...................................................................... $11,000

Architectural design fees......................................................... $8,000

Building permit fee................................................................... $1,000

The building was completed and occupied on September 29, 2016.

A third tract of land (site number 623) was acquired for $600,000 and was put on the market for resale.

Extensive work was done to a building occupied by Kevin under a lease agreement that expires on December 31, 2025. The total cost of the work was $125,000, which consisted of the following:

Painting of ceilings............................................................................. $10,000 (estimated useful life is 1 year)

Electrical work.................................................................................... $35,000 (estimated useful life is 10 years)

Construction of extension to current working area       ..................... $80,000 (estimated useful life is 30 years)                                                                                     $125,000

The lessor, Steinbeck Company, paid one-half of the costs incurred in connection with the extension to the current working area.

During December 2016, costs of $65,000 were incurred to improve leased office space. The related lease will terminate on December 31, 2018, and is not expected to be renewed.

A group of new machines was purchased under a royalty agreement that provides for payment of royalties based on units of production for the machines. The invoice price of the machines was $75,000, freight costs were $2,000, unloading charges were $1,500, and royalty payments for 2016 were $13,000.

Required:

Prepare a detailed analysis of the changes in the balance sheet accounts – Land, Buildings, Leasehold Improvements, and Machinery and Equipment – for 2016. Disregard the related accumulated depreciation accounts.

Please help. Thank you. I included the templates.

Kevin Rocks is a wholesale distributor of automotive replacement parts. Initial amounts taken from Kevin Rocks’ accounting records are as follows:

Accounts Payable at December 31, 2016

Additional information is as follows:

1) Parts held on consignment from Charlie to Kevin Rocks, the consignee, amounting to $155,000 were included in the physical count of goods in Kevin Rocks’ warehouse on December 31, 2016, and in accounts payable at December 31, 2016.

2) $22,000 of parts which were purchased from Full were paid for in December 2016, were sold in the last week of 2016 and appropriately recorded as sales of $28,000. The parts were included in the physical count of goods in Kevin Rocks’ warehouse on December 31, 2016, because the parts were on the loading dock waiting to be picked up by customers.

3) Parts in transit to customers on December 31, 2016, shipped FOB shipping point on December 28, 2016, amounted to $34,000. The customers received the parts on January 7, 2017. Sales of $40,000 to the customers for the parts were recorded by Kevin Rocks on January 3, 2017.

4) Retailers were holding $210,000 at cost ($250,000 at retail) of goods on consignment from Kevin Rocks at their stores on December 31, 2016.

5) Goods were in transit from Greg to Kevin Rocks on December 31, 2016. The cost of goods was $25,000 and they were shipped FOB shipping point on December 29, 2016.

6) A quarterly freight bill in the amount of $2,000 specifically relating to merchandise purchases in December 2016, all of which was still in the inventory at December 31, 2016, was received on January 4, 2017. The freight bill was not included in either the inventory or in accounts payable at December 31, 2016.

7) All of the purchases from Baker occurred during the last 7 days of the year. These items have been recorded in accounts payable and accounted for in the physical inventory at cost before discount. Kevin Rocks’ policy is to pay invoices in time to take advantage of all cash discounts, adjust inventory accordingly, and record accounts payable, net of cash discounts.

Required:

Prepare a schedule of adjustments to the initial amounts of inventory, accounts payable, and sales. Show the effect, if any, of each of the transactions separately and indicate if the transactions would have no effect on the amounts.

4. Below is a proof of Theorem 4.1.1 (b): For the zero vector ⃗0 in any vector space V and k ∈ R, k⃗0 = ⃗0.

   Justify for each of the eight steps why it is true.

   1. k⃗0+k⃗0=k(⃗0+⃗0)

   2. = k ⃗0

   3. k⃗0 is in V

   4. and therefore −(k⃗0) is in V .

   5. It follows that (k⃗0 + k⃗0) + (−k⃗0) = (k⃗0) + (−k⃗0)

   6. and thus k⃗0 + (k⃗0 + (−k⃗0)) = (k⃗0) + (−k⃗0).

   7. We conclude that k⃗0 + ⃗0 = ⃗0

   8. and so k⃗0 = ⃗0, as desired.

Consider the following half reactions at 298 K

Fe²⁺ + 2 e^- → Fe    E^o = -0.441 V
Cd²⁺ + 2 e^- → Cd    E^o = -0.403 V

A galvanice cell based on these half reactions is set up under standard conditions where each solution is 1.00 L and each electrode weighs exactly 100.0 g. How much will the Cd electrode weigh when the nonstandard potential of the cell is 0.02880 V?

The answer is 139g.

Separation of Variables:

Let v(t) be the velocity, in knots per hour, of a vessel at time t, t = 0. After the second engine is turned on, its velocity satisfies the differential equation ??/??=3?+22, with initial condition v (0) = 2.
First use separation of variables to find an expression for v in terms of t. Secondly, f the vessel cruising speed is 25 knots per hour, how long will the vessel take to reach this speed?