The release of product is the rate-limiting for an enzyme. Therefore:

        A) A burst of product formation is expected         C) A catalytic triad is used to bind substrate
        B) transition state binds weaker than product D) DG°’ for the reaction is positive

What does it mean for a molecule to show ALLOSTERIC properties? How does this relate to enzymes? That is, what does it mean if an enzyme is allosterically activated? How about inhibited? What mechanism(s) can explain how this is possible?

Describe why the nitrogenase enzyme complex has to cycle electrons through 3 separate cycles during nitrogen fixation and explain what role ATP hydrolysis plays each time the electrons cycle through the complex.

In your own words (and with the aid of a figure), describe the products of the natural and artificial reactions catalysed by the enzyme. Include in your answer, the special name of the bond that is broken in this type of reaction. Which additional molecule is required to complete this task.

how do you make sure you obtain vectors with the gene of interest and not just some random fragment of DNA that a restriction enzyme also cut out.(both the GOI and the random fragment have the same ends)

You discover a new eukaryotic organism that has a very large genome with DNA bound to histones in the form of chromatin, but has a single circular chromosome. Would this organism be likely to have a telomerase enzyme? Explain your reasoning.

You are looking at an illustration depicting the process of telomere addition. Which enzyme is most likely to be found on the illustration:

Draw and/or describe the process of phototransduction from the molecular change in retinal to the change in ion flow through cGMP-gated ion channels. What would happen if a photoreceptor had a mutation that made the phosphodiesterase enzyme constantly active?