Construct a recombinant bacteria strain with a gene mutated. You need to design and suggest the steps. 1) cloning of gene 2) mutation (how to do it) 3) which homologous recombinant method you use 4) how to verify the strain EXPLAIN THE STEPS IN DETAIL.
In: Biology
Fact:Without thiamine (vitamin B1), the body
cannot convert pyruvate into acetyl-CoA, or use 6 of the 22 amino
acids in the Krebs cycle. Explain in detail why a
person with a thiamine deficiency would have a reduced physical
work capacity.
Detailed answer please!
In: Anatomy and Physiology
Functional Group Identification:
What are the functional groups (alkanes, alkenes, alcohols, aldehydes, ketones, phenol)?
how is reaction with Br2: (and how this can be used to test for alkanes, alkenes with and without light)
Explain Ceric nitrate test (tests for alcohol functional group): turns red in presence of hydroxyl, turns green-brown in presence of phenol.
FeCl3 test: phenols turn red-purple
2,4-DNP (tests for aldehydes and ketones): yellow-red = carbonyl present, but it does not help you decide between the aldehyde and ketone.
Why Tollen’s Reagent: reacts with aldehydes, does not react with ketones, appears to “silver” the solution ?
In: Chemistry
Question, Digital Signal Processing:
a)Explain in detail the difference between FIR filter and IIR filter with examples and responses . Explain briefly?
b)Write a detail note on Least Squares, explain breifly?
NOTE: write the folowing in detaill, thank you.
In: Electrical Engineering
Over the past couple of weeks, there has been tremendous volatility in the global equity markets. Assume that you own an investment portfolio valued at $250,000 on January 1, 2020. What could you have done to protect the value of your portfolio from these market conditions? What type of stock orders or derivatives would you have used and why? Please explain in detail.
In: Finance
Please explain in extensive detail:
What are 5 financial innovations and deregulations that led to the financial crisis in 2008? What are 5 policy responses by the Federal Reserve and the U.S. Government and Treasury department that helped us to get out of the financial crisis? Who are the winners and losers?
In: Finance
1. Please explain, in detail, the following:
a. What is the Fisher effect? Write it out in a mathematical expression and illustrate using supply and demand analysis.
b. Using supply and demand for loanable funds framework, identify FOUR reasons why interest rates have been low in recent years.
In: Finance
Can you explain in detail what Gaussian Elimination with pivoting is? and how is it different from Gaussian Elimination without pivoting?
In: Advanced Math
Please explain in detail what is:
Transport-Facilitated diffusion and how is it involved in the uptake of drugs and how they work on a cell to create an effect on the body.
In: Anatomy and Physiology
explain in detail what crisis management plan is and why it is important for every firm to have one?
500 words/ 2 references in APA format
In: Operations Management