Questions 1
You are the principle investigator of a research unit in the Botany and Plant Biotechnology department that studies medicinal plant usage in South Africa. Your work focused extensively on devil’s claw. You have identified that one of the plant extracts, harpagoside that is an iridoid glycoside, is of medicinal use. You would like to produce this iridoid glycoside in a liquid culture of Escherichia coli at commercial scale. You have identified that the key enzyme that you would need to introduce into E. coli is an iridoid synthase from devil’s claw. This gene is known and studied in Arabidopsis thaliana but remains to be identified from devil’s claw. How would you go about using the known Arabidopsis sequence to isolate the full length gene from devil’s claw? Describe the steps that you will follow in detail and provide all the details of the techniques that you use in each step and how your approach will impact on these steps. Hint: It is critical to remember that devil’s claw is a eukaryote and E. coli, where you want to express the iridoid synthase protein, is a prokaryote. (29)
Question 2
How will you ensure that the PCR amplicon that you have obtained is the correct gene from devil’s claw? Describe the steps that you will follow in detail and provide all the details of the techniques that you use in each step. (11)
Question 3
You want to clone and express the gene that you’ve isolated from devil’s claw in E. coli. Describe all the necessary adjustments that you would need to make to your gene to ensure correct expression in E. coli. (5)
Question 4
Describe all the steps that you would take to clone your gene into E. coli. Draw a diagram of your vector containing the gene insert. Discuss how you will identify a clone in an E. coli cell that you can work further with. Describe the steps that you will follow in detail and provide details of the techniques that you use in each step and how your approach will impact on these steps. (25)
In: Biology
You are a software engineer and project manager, a customer approaches you and wishes you to develop a software product for them
Some details
You have decided to use Agile process:
These are the Agile Process Models
Choose one and explain how it would fit your requirements, also explain how the QA department and the Continuing Engineering department would be involved)
In: Computer Science
Our beliefs influence and ultimately shape the
philosophies that we carry into our classrooms. To assess your
developing beliefs about education, respond to the following
statements, and then answer the questions that follow. Use this
scale in making your responses:
What did the totals of your responses tell you about your beliefs
about teaching?
Did the tallies confirm what you believe your philosophy of
education is at this point in your studies, or were they
inconsistent with what you previously thought your philosophy of
education was?
In: Economics
In a typical business cycle, is consumption or investment more volatile? Explain why.
"Each answer must be about 1 - 2 paragraphs and use economic models/term to explain your answer."
In: Economics
A commonly debated issue (especially during election years) is whether or not to make healthcare and higher education a public good. Use economic concepts and what you have learned about public goods to explain and justify your opinions on whether they should or should not be public goods.
In: Economics
Given what you understand about the price elasticity of demand and supply, explain how the proposed managed isolation fee legislation will affect travel decisions of New Zealanders and NZ permanent residents and how it will affect the airlines. Use suitable diagrams to illustrate your answer.
In: Economics
What is the external environment (Environment factors) facing by Air Asia? Please explain in more detail in at least 3 areas. (No need to explain for all PESTEL, just environment only)
In: Economics
Q No 1: Explain two applications of digital signal
processing in detail.
Q No 2: What is meant by digital signal processor? Explain
architecture of any of the
digital signal processor.
In: Computer Science
Telecommunication Governance 1:
Note:Very Important to add at least 4 bibliographical sources.
Explain in detail, illustrate examples and applications:
What are the types of benefit contributed to the enterprise by IT, explain them.
In: Computer Science
Define with your words a process of excitation of a material by means of radiation, also explain step by step the transition from the initial state to the excited state. What main characteristic can be observed of this excitation process?
In: Physics