This is a confocal microscopy class. So please answer clear and detail according to the subject. Thank you

1.Explain how light diffraction causes the lateral point spread function of light as shown by Airy. Illustrate with a diagram of the PSF in the lateral direction.

2.What is the relative size of the point spread function in the vertical compared to lateral direction and how does this affect the shape of the voxels we record in a optimal resolution z-stack.

3.Is the point spread function wider or narrower with large aperture lenses? Explain why.

4.Does a larger aperture lens have higher resolution (more detail) or lower resolution (poor detail)? Or is aperture size unrelated to resolution?

5.Definedynamicrange.Explaintheimportanceofadjustingthedynamicrangeof the light intensity sensors (PMTs) and how the confocal encodes intensity into numbers.

6.Explain the advantage and disadvantage of frame-averaging.

6b.Explain how to optimize the resolution of your scanned image to avoid oversampling and minimize file size.

7.Make a list of the most functionally-important parts inside of the scan head box and briefly explain what each part does. Hint1: there are 5 kinds of things, and the AOTFs & lasers are not in the scan head box. Hint2: Without these 5 things, it would not be a laser scanning confocal microscope.

8.Explain why immersion oil is used for some lenses. Explain how it works.

9.Explain the relationship between the point-spread function and super-resolution.

10. What is chromatic aberration?

11. How does one detect cross-talk and bleed-through? How can you avoid it, and how can you correct for it if you could not avoid it?

12. Describe the three main ways to manage autofluorescence.

Occupational fraud victims are concerned about how to detect fraud effectively and efficiently. Write an essay on "Occupational Fraud: Detection". In the essay, State and describe the manner in which occupational fraud may be detected, and explain what factors determine the effectiveness and efficiency of the manner of fraud detection.

This assignment is about using attributes in HTML. The attributes you will use for this assignment include the lang attribute, the type attribute, and the start attribute.

For this assignment, you will write an HTML document to create a web page that contains ordered lists with numbers, uppercase letters, lowercase letters, uppercase Roman numerals, and lowercase Roman numerals; and a list with lowercase Roman numerals that starts at a value other than 1.

Your document can be about any topic you would like to write about. For instance, it could be a list of favorite movies or tv shows, favorite foods, etc. You can repeat the same list items for each list (so that you can copy and paste the items without having to re-type them).

Your source code should include:

a DTD

opening and closing html tags

the lang attribute to set the language of the entire document to English

a head section

a title

a body section

a level 1 heading

ordered lists with:

standard numbering

uppercase letters

lowercase letters

uppercase Roman numerals

lowercase Roman numerals

an ordered list with lowercase Roman numerals starting at a value other than 1.

Make sure that your document has all the proper closing tags that go with the opening tags.

Write your source code in a text editor (such as Notepad or TextEdit), and remember to save it with the extension .html or .htm Then open the assignment and upload the file.

Include your last name and the name of the assignment in the filename. Remember to save a copy for yourself, either on your hard drive, or on a flash drive.

Before submitting this assignment, make sure to test your code by opening it in a browser.

Use the Codification to determine the accounting treatment for the following. Two companies engage in a joint venture. Is consolidation required for a joint venture? If so, what factors must exist for consolidation? If consolidation is not required, what is the required accounting treatment for a joint venture? Support your response with a specific reference to the appropriate section of the Codification:

What is shaping? Explain how you could use shaping to get one of your friends to say kind things to you while you are walking to class. Must be at least 150 words, please. (cannot use food of any kind)

Explain in your own words what types of ratios or measures monitoring groups often use to measure whether nonprofit organizations are operating properly, and in the best interests of donors. Then, in your own words, explain why you agree, or don’t agree, that these are useful and appropriate ways to judge nonprofit organizational performance

In your own word, explain why do designers use Denormalization? What is the limitation of using Denormalization? Name and explain a better alternative approach than Denormalization.

Note:

Please I need a short answer only on paragraph and simple words.

Write a short 100 word paper about an issue in your experience that can be resolved using Conservation Laws

USE NO EQUATIONS, USE NO MATH

The essay must describe the issue involved, why it is an issue, both which topic you wish to describe it with (which of conservation laws are applicable) and what subtopic you would use, and adequately explain how the topic is used to resolve or understant the issue.

USE NO EQUATIONS, USE NO MATH

QUESTION 6: For each of the following situations, state the fundamental ethical principle involved (from those listed in SECTION 100 of APES 110).

Situation 1: When tendering for an audit, an auditor made exaggerated claimsconcerning her length of time in the profession.

Situation 2: During the course of an audit of a listed mining company, the audit senior learned about some exciting new discoveries the client had made. He then advised his brother to purchase shares in the company.

Situation 3: An auditor, with no experience in the wine industry, tendered for an audit of a wine company.