You are a researcher at a small biotech company and your company has just obtained the license for use of a human GENOMIC DNA fragment putatively encoding a potentially novel protein, which is thought to regulate p53, the known tumor supressor protein. The scientists who originally cloned this GENE fragment HDM5 "claim" that HDM5 shares 90% DNA sequence homology with one of the HDM2 genes (refer to the review Levine & Oren, 2009). They propose that HDM5 may have HDM2-like properties and may be involved in regulating cell proliferation, and thus a good target to potentially develop as a cancer therapy. Your company has asked you to characterize the gene and gene products, as well as to provide an opinion as to its potential human therapeutic uses.

1. Before proceeding, you wish to know whether this gene is actually expressed in humans (your target market for therapeutic development). ?Describe, in detail, 2 efficient experimental approaches to answer this question. ?Discuss whether your proposed methods measure synthesis or accumulation of the product

3. (10pt) Your colleague professor Stu Dentgenerated a genome-wide DNA methylation map for normal colon cells using MRE-seq (a restriction enzyme approach) and MeDIP-seq(an immunoprecipitation approach). In an intergenic region, he found an interesting locus. This locus is about 20kb. On one end of the locus, there is a 2kb CpG rich stretch that has both relatively lowMRE-seq and MeDIP-seq signals. The rest 18kb has high level of MeDIP-seq signals. (A) (3pt) Why might you suspect that this region encodes for a novel gene? (B) (4pt) You decide to look at histone modification patterns across this regionfor more evidence. There are several genome-wide datasets available for this cell type: H3K4me1, H3K4me3, H3K27me3, H3K9me3, H3K36me3, and H3K9Ac. Which histone mark would youinvestigatefor this locus and why? (C) (3pt) Suggestat least one other source of data (NOT annotation or bisulfide sequencing) that may help you confirm or refute your suspicion, and why you think it may help?

Larry and Moe operate a large restaurant in a busy downtown area of a large city. For breakfast and lunch, they generally have a line of customers waiting. Fire codes prevent them from adding additional seating. The contribution format income statement for the most recent week follows:

Sales (4,960 meals served) $123,008

VC 63, 984

CM $59,024

FC 25,000

NI $34,024

Larry wants to automate food delivery. There would be no more servers. Orders would be placed electronically on iPads located at each table. Food would arrive via an overhead “railroad”. Larry believes that the cost of this system would be $11,200 per week and it would reduce variable costs by $3.00 per person. He thinks that maybe prices could be increased as customers will enjoy the novel environment.

1. Prepare a contribution format income statement using Larry’s predictions. Assume the price and the number of meals served stays the same.

2. Compute the price increase that would be required to achieve a target NI of $40,000 per week.

Mitchell MD, Chivers DP, Brown GE, Ferrari MCO. 2016. Living on the edge: How does environmental risk affect the behavioural and cognitive ecology of prey? Anim Behav. 115: 185-192. Greig EI and Webster MS. 2014. How do novel signals originate? The evolution of fairy-wren songs from predator to display contexts. Anim Behav. 88: 57-65. Petrů M, Špinka M, Charvátová V, Lhota S. 2009. Revisiting play elements and self-handicapping in play: A comparative ethogram of five Old World monkey species. J Comp Psychol. 123(3): 250-263.

1. What two studies examined proximate causes of behaviour? What evidence do the authors provide to support your answer?

2. What two studies examined ultimate causes of behaviour? What evidence do the authors provide to support your answer?

3. Which of the above (1 only) studies examines the evolution of a behaviour? What evidence do the authors provide to support your answer?

Suppose you wish to be outstanding in your field by studying plant cell biology. You discover a new strain of wild lima bean plants from Costa Rica that is resistant to being killed by a hydrophobic pesticide. Which one of the following states the most reasonable hypothesis that could explain the nature of the pesticide resistance?

A) Many chloroplast proteins are encoded in the plant nuclear genome. A nuclear gene encoding an enzyme involved in the dark reactions might confer resistance by degrading the pesticide.

B) In the resistant strain, the plant cell wall might contain a novel phospholipid that binds to the pesticide and prevents its entry into the plant cells.

C) The endoplasmic reticulum in the leaf cells of the resistant strain might contain a mutant version of a cytochrome P450 enzyme that helps to remove the pesticide from the membrane.

D) The resistant strain has mutations in actin that inhibit the development of pluripotent cells in the stem, re. This results in active transport of the pesticide via LDL particles interacting with coated pits on the mitochondrial thylakoid membranes.

E) None of the above

The world post Covid 19 has created the concept of social distancing which will impact on the way we do business. Online purchasing is expected to increase because it will reduce human interaction and contribute to the reduction of Covid 19 related illnesses. However, online purchases have created contemporary legal issues which may be a novel for most legal systems in several Caribbean Countries.

You are required to critically discuss the concept of online purchases and the various methods used in online transactions with supporting case laws.

10 marks

Secondly, critically discuss FIVE (5) CONTEMPORY CASE LAWS related to online purchasing at the International Level, focusing on the judgements handed down in those cases and most importantly how do you think that will impact on the Caribbean online purchaser.

20 marks

Finally, bearing in mind your answers above; what do you think (your opinion) that Caribbean Governments can initiate via its legal systems to enhance and protect online purchasers legal rights?

10 marks

1. In terms of word learning, what is fast mapping?

2. Which of the following is NOT true regarding co-sleeping?​

3. A classic experiment using the three mountains task with 5- and 6-year-old children shows that they

A 35-year-old African American woman36 weeks gestation, gravida 2, para 1100, delivered a 7-pound baby boy 4 hours ago with Apgar scores 7 and 9 at 1 minute and 5 minutes, respectively. The nurse entered the postpartum room and noted that the baby was near the cold window, which was directly under the air-conditioning vent. The nurse suggests to the mother to place the baby in a different part of the room. The mother tells the nurse that she is concerned about her baby. The mother requests that her baby not go back to the nursery because she noticed a bruise on her infant’s bottom and some bloody discharge in her baby’s diaper when she changed the diaper 30 minutes ago. (Learning Objectives 2, 8)

Based on the information above, answer the following questions:

1. Can you explain the newborn’s assessment to the mother regarding any potential abnormalities? What advice would you provide to this new mother?
2. Why did the nurse suggest placing the baby in a different part of the room?

Please provide the Stata commands and outputs where necessary, thank you.

4. The following are data on

y = quit rate per 100 employees in manufacturing

x = unemployment rate

The data are for United States and cover the period 1990-2002.

(a) Estimate the regression and report the results

(b) Construct a 95% confidence interval for β.

(c) Test the hypothesis H₀ : β = 0 against the alternative β=0 at the 5% significance level.

(d) Test Normality of the residuals using Jarque-Bera test.

(e) What is likely to be wrong with the assumptions of the classical normal linear model in this case? Discuss.