Questions
Which of the following is NOT a sequence of DNA that could be cut by a...

Which of the following is NOT a sequence of DNA that could be cut by a restriction enzyme?

a. GAATTC                  b. ATCGAT                 c. GTAC                       d. GTTCCA

e. AGATCT

Why?

In: Biology

Using names and structures show the transformation of pyruvic acid to ethyl alcohol. At the arrows...

Using names and structures show the transformation of pyruvic acid to ethyl alcohol. At the arrows between each step give the names of the enzyme and coenzyme involved.

In: Chemistry

Explain the Enzyme-Based sensors for the assay of glucose. Discuss the reaction mechanism too. Explain in...

Explain the Enzyme-Based sensors for the assay of glucose. Discuss the reaction mechanism too. Explain in detail working principle with the chemical reactions involved in this measurement.

In: Physics

Three classes of proteins are responsible for tagging proteins destined for degradation by the proteasome complex....

Three classes of proteins are responsible for tagging proteins destined for degradation by the proteasome complex. Name the enzymes and briefly describe the function of each enzyme involved.

In: Biology

A plant cell develops a mutation in its Rubisco gene that results in no production of...

A plant cell develops a mutation in its Rubisco gene that results in no production of the enzyme.

What would be the effect on the light reactions, on photosynthesis and on the organism as a whole?

In: Biology

Cell Biology Short Answer Question: Describe some typical features of a restriction enzyme recognition sequence and...

Cell Biology Short Answer Question: Describe some typical features of a restriction enzyme recognition sequence and how blunt and staggered cuts are made.

In: Biology

list the four basic organic molecule types, and for each, describe where it is first meaningfully...

list the four basic organic molecule types, and for each, describe where it is first meaningfully digested in humans and give the enzyme used to break it down.

In: Anatomy and Physiology

The key to increasing the production of ATP is: A. long, slow training sessions. B. PFK...

The key to increasing the production of ATP is:

A. long, slow training sessions.

B. PFK quality.

C. a reduction in the production of LDH.

D. enzyme activity.

In: Anatomy and Physiology

The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site...

The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site at 396 and one HindIII cleavage site at 471. How many bands would you expect to see on the gel if both enzymes were used to digest the enzyme and the digestion was complete? Explain your answer.

Using the same plasmid and restriction enzymes as in question 2, how many bands would you expect to see on the gel if the digestion were partial? A partial digestion means that

not every site on every molecule is cleaved by the restriction enzyme. Explain your answer

In: Biology

There are a number of mitochondrial disorders that affect people with mutations related to enzymes for...

There are a number of mitochondrial disorders that affect people with mutations related to enzymes for the Citric acid cycle, for example:
▪ Fumerase deficiency
▪ Mutations in SUCLA2
▪ Mutations in SUCLG1
▪ Alpha-ketoglutarate dehydrogenase deficiency
o Pick one of these disorders, search the WWW for information on it, and write a paragraph (at least 100 words) in your own words answering the following questions:
▪ What enzyme is affected by the mutation?
▪ Where in the Citric Acid cycle is this enzyme used?
▪ What effect does it have on respiration in the cells?
▪ What symptoms does it produce in the body?

In: Biology