Deficiency of this mitochondrial enzyme impairs heme synthesi

8. You discover an enzyme that catalyzes the reaction: AMP →

→→ → IMP + NH2. Based
on your experiments, you determine that the KM for substrate AMP is

3.0 µ

µµ µM and the kcat = 0.5 sec-1. At 8.0 mM AMP, you determine that the velocity of

the reaction is 0.6 µ

µµ µM/min. What was the concentration of total enzyme, [E]total,

used in this experiment?

1. The “one gene–one enzyme” hypothesis of George Beadle and Edward Tatum was an oversimplification because:

   		A. mutations may not completely inactivate an enzyme.
   		B. some enzymes contain more than one polypeptide.
   		C. some enzymes can be phosphorylated.
   		D. auxotrophs can be corrected.
   		E. some RNAs are independently catalytic.

2. Describe the 5 events that convert an mRNA into a cDNA.

Renin is an aspartyl protease that has a role in the regulation of blood pressure. The active site has two aspartyl residues, one acting as an acid (pK1 5.0) and the other acting as a base (pK2 of 4.0). If the enzyme activity is measured at pH 6.0, what fraction of the enzyme is expected to be ACTIVE

A. 1% B. 9.0% C. 50% D. 90% E. 95% F. 99%

What is CYP450 metabolic system? What did you learn about metabolism of drugs and how that impacts you as a nurse in your work and providing the best care possible? You need to also discuss and give examples of what and how a hepatic inducer enzyme (2 examples) and hepatic inhibitor enzyme (2 examples) act in the body and the effects that will likely result.

Glutamate synthetase is central to nitrate assimilation. You, as a grad student, are curious about how other nutrients might regulate this enzyme; and specifically, you would like to study how the gene encoding this enzyme is regulated by the presences of phosphate in the environment. Describe the design of your experiment(s) that will look at the regulation of the glutamate synthetase gene by phosphate. Remember to include controls in your description.

Propose a 20 amino acid sequence for an amphipathic beta-hairpin strand.