Which of the following are true about transition state analogs?
Group of answer choices
They are molecules.
[ Choose ]FALSETRUE
They are enzymes.
[ Choose ]FALSETRUE
They fit in the active site of the target enzyme.
[ Choose ]FALSETRUE
They bind less tightly than the substrates of the normal enzyme-catalyzed reaction.
[ Choose ]FALSETRUE
They bind more tightly than the products of the normal enzyme-catalyzed reaction.
In: Chemistry
1) How does temperature affect enzyme function? Hint: discuss two separate processes.
2) What is an active site on an enzyme? What is a substrate?
3) What is the metastable state, and what does it have to do with activation energy?
4) What is Vmax, and how does it relate to the concept of saturation?
5) What is allosteric regulation of an enzyme?
In: Biology
Using affinity chromatography, you have attempted to purify an enzyme as a fusion protein that was expressed in E. coli. SDS-PAGE analysis shows that the eluted sample contains the enzyme and it appears to be very pure.
Briefly describe, in general terms, a method you could use to determine the concentration of the enzyme in the eluted sample. Describe the advantages and disadvantages of your approach.
In: Biology
Osteogenesis imperfecta is a disease caused by a mutation in collagen (type X), which causes the infected individual to have brittle bones that easily fracture. Collagen typically has a triple helical structure consisting of three intertwined polypeptide chains (as shown below) with each polypeptide chain having a glycine every third amino acid.
While Osteogenesis imperfecta can be caused by a variety of mutations, a single amino acid change is sufficient to cause certain forms of the disease. If the mutation resulted in the glycine being changed to another amino acid, which amino acid do you think would be most likely be responsible for this disease? In a sentence, explain your choice.
Write down both the tRNA and coding DNA sequence for the amino acid found in the wild-type protein. Make sure to label the 5’ and 3’ ends.
Where in the cell would collagen be produced? Briefly explain why.
In: Biology
explain why the carbons of even chain fatty acids can never be used to synthesize NET glucose, but that the 14C from labeled fatty acids will be found in glucose.
In: Chemistry
In: Chemistry
In worms, the genes for the production of the enzyme Aldolase and the enzyme Hyclase are on the same chromosome. A = dominant, can produce Aldolase; a = recessive, cannot produce Aldolase. H = dominant, can produce Hyclase; h = recessive, cannot produce Hyclase.
You cross an AaHh worm with an aahh worm and get the following progeny:
16 Wild type
76 Do not produce Aldolase
82 Do not produce Hyclase
12 Produce neither Aldolase nor Hyclase
A) (3 pts) What is the haplotype for the AaHh parental worm? Explain your answer
B) (3 pts) What is the haplotype of the 16 wild type worms in the F1 generation? Explain your answer
C) (6 pts) What is the map distance between the aldolase and hyclase genes? Explain your work.
In: Biology
1. A photoautotroph is an organism that
-Is a decomposer.
-Must obtain organic compounds for its carbon needs.
-Gets energy from sunlight.
-Gets energy by oxidizing chemical compounds.
-Does not need a carbon source.
2. An organism that is harmed by the presence of oxygen in the environment is a(n)
-Aerobe.
-Obligate aerobe.
-Facultative anaerobe.
-Microaerophilic.
-obligate anaerobe
3. Formation of peptide bonds between amino acids to build a polypeptide would be called
-Anabolism.
-Phosphorylation.
-Fermentation.
-Exergonic.
-Glycolysis.
4. Each of the following are denaturing agents except
-High temperature.
-Low temperature.
-High pH.
-Low pH.
-All of the choices are correct.
5. An organism that uses CO2 for its carbon needs would be called a(n)
-Heterotroph.
-Autotroph.
- Chemoheterotroph
-Saprobe.
- Halotroph
6. During replication, each parent DNA strand serves as a _____ for synthesis of new DNA strands.
-copy point
-template
-comparison molecule
-scaffold
-reservoir
7. In bacterial cells, when glucose is completely oxidized by all the pathways of aerobic cellular respiration, what is the maximum number of ATP generated?
-2 ATP
-3 ATP
-24 ATP
-42 ATP
-38 ATP
8. Replication of DNA begins at a(n) ______ rich area.
-Guanine-cytosine.
-Uracil-adenine.
-Adenine-thymine.
-Adenine-cytosine.
-Guanine-adenine.
9. Which of the following is not associated with a bacterial ribosome?
-is a 70S
-has a peptide (P) site
-has an exit site
-small unit provides the enzymes for making peptide bonds
-has an amino acid (A) site
10. Human pathogens fall into the group
-Psychrophiles.
-Thermophiles.
-Halophiles.
-Mesophiles.
-Acidophiles.
11. A sequence of bases on a gene that does not code for protein is called a(n)
-Promoter
-Operator
-Operon
-Exon
-Intron
12. A mutation that changes a normal codon to a stop codon is called a
-Point mutation.
-Silent mutation.
-Back mutation.
-Missense mutation.
-Nonsense mutation.
13. All of the following are true of enzymes except
-Enzymes increase the rate of a chemical reaction.
-Enzymes are not part of the products of a chemical reaction.
-Enzymes create a chemical reaction.
-Enzymes are not consumed in a chemical reaction
14. A frame shift is caused by ______ mutations.
-Missense and insertion
-Missense and nonsense
-Nonsense and deletion
-Deletion and insertion
-Insertion and nonsense
15. What is the definition of Generation Time
16. Vitamins are important components of coenzymes
True
False
17. DNA replication proceeds in one direction around the bacterial chromosome
True
False
18. Enzymes that hydrolyze a peptide bond are called ligases.
True
False
19. Transposons can change the genome
True
False
20. Ribosomes are composed of ribosomal RNA and protein.
True
False
21. The jumping of a gene from one location to another is done by transposons.
True
False
22. Transformation is a type of horizontal gene transfer
True
False
23. Bacterial conjugation involves a donor cell with a plasmid that synthesizes a pilus.
True
False
24. Transcription occurs on a ribosome in the cytoplasm.
True
False
25. Full induction of the lactose operon requires lactose present without glucose.
True
False
26. A microorganism that does not have catalase or superoxide dismutase would find it difficult to live in an environment with _______
27. An organism that requires a small amount of oxygen but won't grow at normal atmospheric levels is a __________
28. A permanent, inheritable change in the genetic information is called a _________
29. For DNA replication, a primer of ____ is needed at the origin of nucleotide addition.
30. An organism that can exist in both oxygen and oxygen less environments is a ____________________.
31. The property of organisms to integrate catabolic and anabolic pathways to improve cell efficiency is called ______
32. If the mRNA codon for alanine is GCA, then the correct anticodon would be ___.
33. An example of inducible operon is the _____ operon.
34. Reverse transcriptase is used to make ______ from an RNA template.
35. Environmental factors that influence microbial growth are (all that apply)
-temperature
-gases
-pH
-osmotic
-pressure
-radiation
-hydrostatic
-pressure
36. The three main types of symbiosis are ____, _____, and _____.
-amphibolism, commensalism and parasitism.
-anabolism, catabolism and metabolism.
-mutualism, commensalism and parasitism.
-catabolism, metabolism, and parasitism.
37. The two general categories of metabolism are ____ and ____.
-anabolism and catabolism.
-mutualism and parasitism.
-commensalism and parasitism
-anabolism and parasitism.
38. ______ refers to the process of one bacterial cell becoming two.
39. At the ____ growth phase, the population enters a period during which the rates of cell birth and cell death are more or less equal.
In: Biology
almost three-fourths of us pecans are grown in the
states of GA,NM, and TX it is known that the average yield of
pecans in DA county is mean=1625 and standard dev.= 200 if a random
sample of 18 acres of a land is taken the probability of the
average yield of these 18 acres being at most 1700 lbs would
be
.9941?
In: Statistics and Probability
Evaluate the following integral ∫ ∫ R 4x + y (3x − y) ln(3x − y) dA where R is the region bounded by the graphs of y = 3x − e7, y = 3x − e5, y = −4x + 8, and y = −4x + 5. Use the change of variables u = 3x − y, v = 4x + y.
In: Math