Basically what would be the effect of using O2 instead of NAD+ in glycolysis? Is NAD+ more or less favorable to use in this pathway?

Why does ATP yield vary in cellular respiration (think about how NADH from glycolysis enters mitochondira)?

Which of the following are true about transition state analogs?

Group of answer choices

They are molecules.

[ Choose ]FALSETRUE

They are enzymes.

[ Choose ]FALSETRUE

They fit in the active site of the target enzyme.

[ Choose ]FALSETRUE

They bind less tightly than the substrates of the normal enzyme-catalyzed reaction.

[ Choose ]FALSETRUE

They bind more tightly than the products of the normal enzyme-catalyzed reaction.

1) How does temperature affect enzyme function? Hint: discuss two separate processes.

2) What is an active site on an enzyme? What is a substrate?

3) What is the metastable state, and what does it have to do with activation energy?

4) What is V_max, and how does it relate to the concept of saturation?

5) What is allosteric regulation of an enzyme?

Using affinity chromatography, you have attempted to purify an enzyme as a fusion protein that was expressed in E. coli. SDS-PAGE analysis shows that the eluted sample contains the enzyme and it appears to be very pure.

Briefly describe, in general terms, a method you could use to determine the concentration of the enzyme in the eluted sample. Describe the advantages and disadvantages of your approach.

Osteogenesis imperfecta is a disease caused by a mutation in collagen (type X), which causes the infected individual to have brittle bones that easily fracture. Collagen typically has a triple helical structure consisting of three intertwined polypeptide chains (as shown below) with each polypeptide chain having a glycine every third amino acid.

While Osteogenesis imperfecta can be caused by a variety of mutations, a single amino acid change is sufficient to cause certain forms of the disease. If the mutation resulted in the glycine being changed to another amino acid, which amino acid do you think would be most likely be responsible for this disease? In a sentence, explain your choice.

Write down both the tRNA and coding DNA sequence for the amino acid found in the wild-type protein. Make sure to label the 5’ and 3’ ends.

Where in the cell would collagen be produced? Briefly explain why.

In worms, the genes for the production of the enzyme Aldolase and the enzyme Hyclase are on the same chromosome. A = dominant, can produce Aldolase; a = recessive, cannot produce Aldolase. H = dominant, can produce Hyclase; h = recessive, cannot produce Hyclase.

You cross an AaHh worm with an aahh worm and get the following progeny:

16 Wild type

76 Do not produce Aldolase

82 Do not produce Hyclase

12 Produce neither Aldolase nor Hyclase

A) (3 pts) What is the haplotype for the AaHh parental worm? Explain your answer  

B) (3 pts) What is the haplotype of the 16 wild type worms in the F1 generation? Explain your answer

C) (6 pts) What is the map distance between the aldolase and hyclase genes? Explain your work.

almost three-fourths of us pecans are grown in the states of GA,NM, and TX it is known that the average yield of pecans in DA county is mean=1625 and standard dev.= 200 if a random sample of 18 acres of a land is taken the probability of the average yield of these 18 acres being at most 1700 lbs would be
.9941?

Evaluate the following integral ∫ ∫ R 4x + y (3x − y) ln(3x − y) dA where R is the region bounded by the graphs of  y  =  3x − e7,  y  =  3x − e5,  y  =  −4x + 8,  and  y  =  −4x + 5. Use the change of variables  u  =  3x − y,  v  =  4x + y.