4. The rate with which enzymes can transform a substrate varies from enzyme to enzyme, and sometimes substrate to substrate. a) How are competitive and uncompetitive reversible inhibition similar? How is their effect on rate different? b) Enzyme reaction rates drop off dramatically as temperatures pass 50 °C or so, and do not return to their previous rate when cooled. Explain the drop off in activity and the lack of return to the previously higher reaction rates after heating. c) Pepsin is an enzyme that participates in digestion in the stomach, whereas trypsin is an enzyme that participates in digestion in the small inestines. Pepsin has the highest turnover number at around pH 2, whereas trypsin has the highest turnover number around pH 8. Explain this difference.
In: Chemistry
You are given 1.0 mL of the enzyme amylase and add cold 95% ethanol to reach a final concentration of 30% ethanol. What volume of the 95% ethanol did you need to add to the enzyme solution to obtain this concentration? (*note: this one is a little tricky—you will need to account for the starting volume of the enzyme solution.)
In: Chemistry
please answer in excel format
In: Finance
ch 18 # 2
Four identical metal spheres have charges of
qA = -8.0 μC, qB=-2.0 μC,
qC=+5.0 μC, and qD=+12.0
μC.
(a) Two of the spheres are brought together so
they touch, and then they are separated. Which spheres are they, if
the final charge on each one is +5.0 μC?
Choose the answer for part (a) from the menu in accordance to the
question statement
C and DB and DB and CA and BA and DA and
C
(b) In a similar manner, which three spheres are
brought together and then separated, if the final charge on each of
the three is +3.0 μC?
Choose the answer for part (b) from the menu in accordance to the
question statement
A+C+DB+C+DA+B+CA+B+D
(c) The final charge on each of the three
separated spheres in part (b) is +3.0 μC. How many
electrons would have to be added to one of these spheres to make it
electrically neutral?
N = Number
In: Physics
The purpose of this assignment is to learn how to apply the t-test to a sample dataset.
For this assignment, students will use IBM SPSS Statistics and the "Example Dataset."
Using the "Example Dataset" and SPSS, apply the t-test to assess the following statement: "Men and women have different incomes in this city."
Show your calculations and copy of the SPSS output in a Word document.
In a separate 250-500 Word document, address the following questions:
|
Annual_Income* |
Sex |
|
51000 |
Female |
|
23000 |
Male |
|
35000 |
Female |
|
10000 |
Male |
|
28000 |
Female |
|
5000 |
Female |
|
46000 |
Female |
|
36000 |
Male |
|
51000 |
Female |
|
12000 |
Male |
|
78000 |
Female |
|
34000 |
Male |
|
15000 |
Female |
|
28000 |
Male |
|
28000 |
Male |
|
24000 |
Male |
|
55000 |
Female |
|
62000 |
Male |
|
32000 |
Male |
|
7000 |
Female |
|
17000 |
Female |
|
64000 |
Female |
|
5000 |
Male |
|
14000 |
Male |
|
20000 |
Female |
|
72000 |
Male |
|
85000 |
Female |
|
15000 |
Female |
|
64000 |
Male |
|
27000 |
Male |
In: Statistics and Probability
The data for an Enzyme with a Kcat is 5,000m-1. This experiment was performed by adding a given amount of substrate of 100 micrograms of enzyme in buffer solution. What is the molecular weight of the enzyme?
| Substrate Concentration mM | rate (Vo) micromol/min |
| 1 | 167 |
| 2 | 250 |
| 4 | 334 |
| 6 | 376 |
| 100 | 498 |
| 1000 | 499 |
In: Chemistry
An enzyme contains an active site aspartic acid with a pka=5.0 which acts as a general acid catalyst. Draw the curve of enzyme activity vs. pH for the enzyme (assume the protein is stably folded between pH 2-12 and that the active site Asp is the only ionizable residue involved in catalysis).Explain the shape of the curve
In: Chemistry
Describe what is happening during glycolysis. Make sure you include all compounds made and in the correct order.
In: Biology
A strict anaerobe can gain its energy from:
Chemolithotropy
anaerobic respiration
glycolysis
fermentation
all are correct
In: Biology
Which of the following stages of cellular respiration makes the most ATP?
|
glycolysis |
||
|
citric acid cycle |
||
|
oxidative phosphorylation |
In: Biology