PLEASE TYPE OUT YOUR WORK
1A)Explain the difference between primary, secondary, tertiary, and quaternary structure of proteins, including similarities and differences in hydrogen bonding of EACH STRUCTURE WITH ONE ANOTHER
1 B) Distinguish between parallel and antiparallel BETA-sheets
1C) which kinds of forces stabilize proteins at different levels of structure, i.e., what stabilizes secondary structure, what causes protein chains to fold into tertiary structure, what holds oligomers together?
1D) which bonds can rotate in proteins, and WHAT ARE the names of the angles describing that rotation
1E) . Describe what an enzyme assay is, and how it is used in a protein purification.
In: Biology
3. Mrs. Natasha gave birth to a boy child and her friend Mrs.
Tina gave birth to a female child. When the babies are born, the
environment is different both inside and outside of the body. Based
on the above case answer your questions. (2.5 Marks)
a) How do the new born girl and boy babies are coping up with new
environment?
b) What type of enzyme and neurotransmitters are involved with this
process?
c) Is there any difference between these babies in coping up with
the new environment? Comment on it.
d) Which type of tissue is involved in this process?
e) Mention the name of the gas which is involved in this
process.
f) Write any two locations of this tissue.
In: Biology
Your colleague has isolated a mutant bacterial strain and found the dna 5x10^8 bp in length. she has asked you to do restriction mapping. you start by determining the base composition of the bacterium, and find that the bacteria consists of 24% Thymine and 26% Guanine. you plan on using the enzyme NOTI which recognizes the sequence 5'-GCGGCCGC-3'
a) How many fragments would you expect assuming that it occurs randomly throughout the genome
b)your colleague also provided several variants of her strain of bacteria. You test all of them and found differences in their respective banding pattern, in a simple statement, what could account for this?
In: Biology
| Enzyme Used | Lysozyme |
| Thermal Denaturation | |
| Temp (Celsius) | Abs (325 nm) |
| 40 | 0.106 |
| 50 | 0.086 |
| 60 | 0.109 |
| 65 | 0.161 |
| 70 | 0.409 |
| 75 | 2.06 |
| 80 | 2.173 |
| 90 | 0.821 |
| Urea Denaturation | |
| M of urea | Abs (325 nm) |
| 0 | 0.464 |
| 1 | 0.07 |
| 2 | 0.056 |
| 4 | 0.05 |
| 6 | 0.035 |
| 8 | 0.028 |
Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find ΔH, ΔS and ΔG(T=75) (mention any assumptions you made) and What is the Tm for each protein
In: Chemistry
1)
Which of the following are TRUE about the process of translation? (Select all that apply)
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Transcription factors attach to the mRNA to promote its circularization in the cytoplasm |
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Binding of the large ribosomal subunit is the end of the initiation stage |
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The third base of a codon permits wobble pairing |
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Charged tRNAs enter the P site prior to being added to the protein chain |
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There are three stages: initiation, elongation, and termination |
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The process cannot be regulated until after the protein chain is complete |
2)
Which of the following biological tools is necessary for transferring a gene from one source of DNA to another?
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Next-gen sequencing |
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Restriction enzyme |
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DNA polymerase |
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Histone acetylase |
In: Biology
1.The TCA cycle in a particular tissue has been inhibited by fluoroacetate. However, it is not the fluoroacetate that is responsible for the inhibition. It gets converted to another molecule that enters the TCA cycle and is transformed into the inhibitor.
a) Propose the inhibitor molecule and mechanistically show the conversion of fluoroacetate to the inhibitor.
b) Indicate at what step fluoroacetate ultimately inhibits the TCA cycle.
c) Explain why you think fluoroacetate acts as an inhibitor of this enzyme? Your answer should include some sort of mechanistic explanation.
d) What difference in the concentration of each TCA cycle metabolite would you expect, compared with a normal uninhibited tissue? List each metabolite and indicate the effect on its concentration.
In: Biology
Why is kcat not necessarily the best way to evaluate or compare the activities of two enzymes? What “adjustment” can be made to make the term more useful and why? A) Define the rate constants k1, k2 and k-1. Given k1 of 2x10^-8/M s, k2 of 5x10^3/s, and k-1 of 1x10^3/s, what is KD? what is KM? B) Which substrate is preferred: R, for which enzyme X has a KM of 2mM? OR Q, for which X has a KM is 7mM? Explain. If k2 for Q is 2x10^4/s and for R is 4x10^5/s, which substrate yields the best catalytic efficiency? Explain.
In: Chemistry
1) The Liberal leader, Mr. Wilkinson, refers to the privatization of the ICBC during the debate. How does this political action refer to the liberal perception of human nature?
In: Economics
a) Under what conditions might increase in average Incomes Per Capita not translate into greater human development?
b) Give three specific examples and explain.
In: Economics
Describe the role of the U.S. Department of Health and Human Services and list all of it's divisions with a description of the role of each. Be sure to cite all references used.
In: Nursing