If cloning of a 0.8 kb DNA segment was performed at the PstI site of plasmid pBR322 and the E. coli strain HB-101 was transformed with the construct:
a) What is the selection criteria for E. Coli transformants with the recombinant plasmid ?:
-Ecoli growth in nutrient-rich culture medium____
-Ecoli growth in culture medium rich in nutrients plus ampicillim_____
- Growth of E. coli in a culture medium rich in nutrients plus tetracycline_____
-Ecoli growth in culture medium rich in nutrients plus ampicillim and no growth in tetracycline_____
-Ecoli growth in culture medium rich in nutrients plus tetracycline and no growth in ampicillim_____
b) How would it be verified that the selected transformant has the recombinant molecule, if it is known that the cloned DNA segment does not have restriction sites with PstI?

An economy is described by the following equations:

a. When Y = 600, aggregate expenditure is .

b. Consumers and businesses alike become more pessimistic about the future and reduce their respective expenditures by 10 each. Immediately following this change, what is aggregate expenditure if Y is still 600? (In other words, what is the initial change in aggregate expenditure?)

The new aggregate expenditure is

In order to offset the initial drops and avoid a recession, government purchases should be change by .

e. If the multiplier effect were allowed to take effect, then government purchases would have to change by .

An economy is described by the following equations:

The multiplier in this economy is 5.

a. Find a numerical equation relating planned aggregate expenditure to output.

Instruction: Enter your response for mpc rounded to one decimal place.

PAE = + Y.

b. Construct a table to find the value of short-run equilibrium output.

Instruction: If you are entering any negative numbers be sure to include a negative sign (-) in front of those numbers.

Short-run equilibrium output is .

c. By how much would government purchases have to change in order to eliminate any output gap? By how much would taxes have to change?

Instruction: Enter your responses rounded to one decimal place.

In order to eliminate any output gap, government purchases would have to be (Click to select)reducedincreased by .

In order to eliminate any output gap, taxes would have to be (Click to select)reducedincreased by .

d. If Y* = 1,100, then by how much would government purchases have to change in order to eliminate any output gap? By how much would taxes have to change?

Instruction: Enter your responses as integer values.

In order to eliminate any output gap, government purchases would have to be (Click to select)reducedincreased by .

In order to eliminate any output gap, taxes would have to be (Click to select)increasedreduced by .

a 0.16 kg mass at the top of a 0.8 m high (vertical) incline. if this mass were a uniform, solid sphere (radius .057 m, like a billiard ball) and it is allowed to roll (without slipping) down the incline, what will be its speed (in m/s) at the bottom of the incline?

A 120 kg crocodile swallows gastrolithes that are 0.8% of his mass to sink to a lower level. How much would it have to increase its lung volume in mL to counteract the sinking effect? Assume that the air pressure in its lungs is not much greater than atmospheric pressure.

PORTFOLIO BETA

An individual has $45,000 invested in a stock with a beta of 0.8 and another $30,000 invested in a stock with a beta of 2.5. If these are the only two investments in her portfolio, what is her portfolio's beta? Round your answer to two decimal places.

Assume that a hypothetical economy with an MPC of 0.8 is experiencing severe recession. By how much would government spending have to rise to shift the aggregate demand curve rightward by $40 billion? How large a tax cut would be needed to achieve the same increase in aggregate demand? Determine one possible combination of government spending increases and tax increases that would accomplish the same goal without changing the amount of outstanding debt (i.e., maintaining the budget balance at its current value).

A four-stroke engine is running on methane with an equivalence ratio of 0.8. The air and fuel enter the engine at 250 C, the exhaust is at 5270 C, the heat rejected to the coolant is 340 MJ/kmol of fuel.(a)Write the equations for stoichiometric and actual combustion.(b)By calculating the enthalpy of the exhaust flow, deduce the specific work output of the engine, and thus the overall efficiency. (c)If the engine has a swept volume of 5 Litres, and volumetric efficiency of 72%, calculate the power output at engine speed of 1500 rpm. The ambient pressure is 1 bar.State clearly any assumption that you make, and comment on any problem that would occur with a rich mixture.

Assume that a hypothetical economy with an MPC of 0.8 is experiencing severe recession.

Instructions: In part a, round your answers to 2 decimal places. Enter your answers as positive numbers. In part b, enter your answers as whole numbers.

a. By how much would government spending have to rise to shift the aggregate demand curve rightward by $30 billion?

How large a tax cut would be needed to achieve the same increase in aggregate demand?

b. Determine one possible combination of government spending increases and tax increases that would accomplish the same goal without changing the amount of outstanding debt (i.e., maintaining the budget balance at its current value).

increase government spending by ____ billion

increase taxes by ____ billion

A stock is trading for 15, and just paid a dividend of 0.8 which is expected to grow at a fraction 0.08 per year. If Goldman Sacs charges a fraction 0.10 as a flotation cost, what is the required rate of return on a new stock issue?