A company reports the following:
| Tax rate = 35% | 2012 | 2013 | 2014 |
| Taxable income | 2,000 | 2,500 | -1,500 |
| Taxes paid | -700 | -875 | 0 |
How much of a tax refund will be received in 2012 and 2013?
| a. |
2012: refund = $700; 2013: refund = none |
|
| b. |
2012: refund = 700; 2013: refund = 875 |
|
| c. |
2012: refund = 525; 2013: refund = 525 |
|
| d. |
2012: refund = 525; 2013: refund = none Net income is 1,012. Interest expense totals 170, while EBITDA is 2,466. If taxes are 646, what is depreciation and amortization (DA) ? Beginning equity for a company is 187, and ending equity is 204. The company sold stock in the amount of 121, and net income is 81. Given this information, how much did the company pay in dividends this year? A business purchases depreciable equipment for 207, and sells it a few years later for 169. At the time of the sale, accumulated depreciation totals 101. If the company's tax rate is 33, what is the total after tax cash flow that will result from selling this asset? |
In: Accounting
You want to set-up a restriction enzyme digestion of a plasmid DNA sample. You would like to digest 0.75ug of this DNA with 50 units of BamHI and 50 units of HindIII in a total volume of 25uL containing 1x buffer A.
How much (in uL) of the following stock solutions and water are needed?
a) 200ng/uL plasmid DNA
b) 10x buffer A
c) 50,000 units/mL BamHI
d) 25,000 units/mL HindIII
e) water
In: Biology
8) Which of the following reactions with covalently closed, circular double stranded DNA (ccc dsDNA) does NOT result in a new linking number? Select one choice from the list below (A-D) and explain your answer.
A. incubation with a type I topoisomerase
B. incubation with a type II topoisomerase
C. incubation with ethidium bromide
D. a brief incubation with DNase I followed by resealing the breaks in the phosphodiester backbone with another enzyme (DNA ligase)
In: Biology
Q2. An enzyme has a Km of 2.8 * 10-5 M and a Vmax of 53 μM/s.
(i) Calculate Vo if [S] = 3.7 * 10-4 M and [I] = 4.8 *10-4 M for (a) a competitive inhibitor, (b) a noncompetitive inhibitor and (c) an uncompetitive inhibitor. (Assume Ki = 1.7 * 10-5 M.)
(ii) The degree of inhibition is given by i (%) = 100(1 - Vi/Vo). Calculate the percent inhibition in each of the three cases above.
In: Chemistry
An uncatalyzed reaction has a rate of 4.4 x 10-7 s–1 at room temperature (25 °C). When an enzyme is added the rate is 3.3 x 104 s–1. If the height of the activation barrier for the uncatalyzed rate is 28 kcal/mol, what is the height of the activation barrier for the catalyzed rate? Report your answer in terms of kcal/mol to the nearest tenths. Also, assume the pre-exponential terms for the uncatalyzed and catalyzed reactions are the same.
In: Chemistry
3. Cellular transport of proteins is very important in order for a cell to function properly. For each of the mutations below, (1) explain what type of transport will be affected by explaining the correct transport process and where the problem will arise and (2) where the proteins that are transported incorrectly will be found (6 pts). (1) A mutation in N-acetyl transferase, the enzyme that adds N-linked glycosylation to proteins. (2) A mutation in the TOM complex (3) A mutation that causes GTP to not be hydrolyzed in the cytosol
In: Biology
pls ansr a-e for thumbs up T/F questions about electron transport
a. total of 10 protons will be pumped from N to P side when electrons move from NADH to oxygen
b. glycol 3 phosphate shuttle is one of many sources of electron that can reduce co enzyme Q
c. electron from NADH must travel through complex I, II , III,
before going to oxygen at complex IV
In: Biology
Methods – What is the procedure? What is measured? Why would it be used?
1. Southern blot analysis
2. Restriction enzyme digestion
3. Primer extension
4. Electron microscopy
5. PAGE
6. PCR
7. Western blot
8. Gel mobility shift assay
9. Immunoprecipitation
10. ChIP
11. Plasmid vector
12. Making transgenic mice
13. Microinjection
14. Electroporation
15. cDNA synthesis
16. Photolithography
In: Biology
Bacterial operons & regulating metabolic pathways
Two kinds of control: enzyme regulation vs. gene regulation. Why?
trp operon. How does the presence of tryptophan prevent the synthesis of more tryptophan (2 different ways)?
lac operon. Compare & contrast with trp.
Why are operons for catabolic and anabolic pathways regulated differently?
Positive vs. negative gene regulation. Why would both be used for the same operon?
AraC mechanism. Is AraC an inducer or a repressor?
In: Biology
Outline the roles played by restriction enzymes and vectors in cloning DNA.
Words to choose from: bacterial, eukaryotic, nucleotide, DNA, RNA, protein, palindrome,
nuclease, protease, phage, plasmid.
1. A restriction enzyme is a _________ ____________that recognizes specific _________ sequences in a ________molecule, often a __________, and cleaves or nicks the molecule at those sites.
2. A vector is an agent into which a foreign DNA segment is inserted and used to transform host cells. A vector can be created from a virus known as a __________ or a bacterial _________.
In: Biology