Discuss current issues in healthcare human resources from a global perspective, countries such as the USA, Canada and Germany just to name a few. Select 2 countries and compare the current state of healthcare human resources for each you have selected. Your response should be 1 - 2 pages.

You are an IT company and want to get a daycare's network design, hardware, software, and security. Project resources allocation. List all types of resources (e.g. human and non-human) you will use them in the enterprise network project. How are you planning to use those resources cost-effectively?

You are an IT company and want to get a daycare's network design, hardware, software, and security. Project resources allocation. List all types of resources (e.g. human and non-human) you will use them in the enterprise network project. How are you planning to use those resources cost-effectively?

Amyloidosis is caused by an abnormal deposition and accumulation of protein aggregates in tissues. How do proteins adopt and maintain a stable folded structure? What features of the protein amino acid sequence determine the stability of the folded structure? How does disruption of that structure lead to protein deposition diseases such as amyloidosis, Alzheimer's disease, and Parkinson's disease? Please respond to at least two classmates with additional questions and comments to further the discussion.

Please no handwritten or picture responses - only typed replies.

1.Ansawer the following questions about chemical synthesis of Peptides: A. What groups must be blocked in chemical synthesis of peptide? B. In what order are the amino acidss added? C. Described Step-by-step how to chemically synthesize a dipeptide.    2. what are the two main methods used to inhibt proteases after cell lysis? 3. List three ways proteins can be denatured once a cell is broken open. 4. Explain the journey of mRNA molecule through the secretory pathway.

Gram-negative bacteria are surrounded by two membrane bilayers separated by a space termed the periplasm. The periplasm is a multipurpose compartment separate from the cytoplasm whose distinct reducing environment allow certain key protein structural features to be formed. Can you identify an amino acid(s) that would be affected by this reducing environment? How would it be affected, and what structural features would be sensitive to this environment? Can you discuss the implications of this from a standpoint of recombinant protein expression? (500 words)

A variation of the acetamidomalonate synthesis can be used to synthesize threonine. The process involves the following steps: Ethoxide ion deprotonates diethyl acetamidomalonate, forming enolate anion 1; Enolate anion 1 makes a nucleophilic attack on acetaldehyde, forming tetrahedral intermediate 2; Protonation of the oxyanion forms alcohol 3; Acid hydrolysis yields dicarboxyamino alcohol 4; Decarboxylation leads to the final amino acid. Write out the mechanism on a separate sheet of paper, and then draw the structure of tetrahedral intermediate 2.

1. What sorts of residues (i.e. what amino acid side chains) would one expect to find on the surface of a protein with an unusually high isoelectric point (e.g. 9.5). What if the pI were unusually low (e.g. 3.4)?

2. When one talks about “protein-ligand” interactions, what sorts of molecules can be “ligands?”

3. How would you explain the conformational selection model of protein-ligand interactions to an educated lay person?

Both myoglobin and hemoglobin exhibit cooperative binding to oxygen

true or false

Tropocollagen is a double helix of two left handed polypeptide chains

true or false

F actin is a polymer of G actin monomers and exhibits symmetry

true or false

Proteins have an asymmetrical tertiary structure, while multisubunit proteins can exhibit several types of symmetry

true or false

When referring to the amino acid sequences of proteins, sequence homology is the same as sequence similarity.

true or false

a. Identify hydrogen bond donors and acceptors in each Watson-Crick base pair. Why won't adenine base hydrogen bond with cytosine or guanine hydrogen bond with thymine?

b. Name an important structure stabilized by Van der Waals interactions. Explain how the Van der Waals interactions work in this instance

c. Explain how the ionization state of any amino acid (or its side chain in a protein) at any

pH can be predicted, based on its pKa value.