You are trying to establish the IgG response from a recently vaccinated individual, where it will be important to know how much IgG they have both before and after the vaccination. You start with two samples of 8 mL of blood, one from before vaccination and one after. You use an affinity chromatography technique to remove just the IgG (ε = 1.35 g-1L cm-1). You end with a 2 mL sample of each. The pre-vaccinated sample has an A280 reading of 0.85, and the post-vaccinated sample with a 10-fold dilution has an A280 reading of 0.202 (both with a 1 cm path length).
A) How many micrograms of IgG were in each of the original blood samples?
B) What fold change in IgG amounts was seen after vaccination? (this question is asking how much ending IgG was there as compared to how much starting IgG)
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You are trying to establish the IgG response from a recently vaccinated individual, where it will be important to know how much IgG they have both before and after the vaccination. You start with two samples of 8 mL of blood, one from before vaccination and one after. You use an affinity chromatography technique to remove just the IgG (ε = 1.35 g-1L cm-1). You end with a 2 mL sample of each. The pre-vaccinated sample has an A280 reading of 0.85, and the post-vaccinated sample has an A280 reading of 0.202 with a 10-fold dilution (both with a 1 cm path length).
a) How many micrograms of IgG were in each of the original blood samples?
b) What fold change in IgG amounts was seen after vaccination?
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