BIOCHEMISTRY

1) consider the following 2 scenarios and answer:

a) When you isolated a hot thermal vents of the ocean floor and another soil bacteria that lives in cold dark place. which one will have the higher GC content and why?

b) In a double stranded RNA containing 40% cytosine, spontaneous deamination occurs and 20% of the cytosine is converted to uracil. A repair enzyme comes and fills the correct complement for the Uracil. what will be the melting point of the coded RNA?

2) The enzyme succinate dehydrogenase acts on succinate and convert it to fumarate tn the TCA cycle. In the presence of malonate the apparent km reduced but the vmax remains unaltered. What can you conclude about the type of inhibition by malonate and the structure of malonate?

3) A short fragment of a double -stranded DNA is heated to 60 degress celsius and the student monitirs the reaction by UV spectroscopy. What will happen to the absorbance when th temperature increased and why?

A biochemist studying the activity of the enzyme triose phosphate isomerase (TPI) in a species of Drosophila wishes to know if the mean activity of this enzyme is the same at pH = 5 and at pH = 8.

Assuming activities in µM/min are normally distributed, determine if the mean activities of TPI are significantly different at these two pH levels.

Suppose that you mistakenly believed that the data sets were independent samples taken from two different populations, that is, two different species of Drosophila.

Carry out the t test and determine whether you would have found that female dementia patients who were given bright light and melatonin would have a significantly higher subjective scores for well-being.

Explain the cause of any differences in your results when compared to the previous problem.

(1) Answer the three questions for each of the following transcription factors:

LacR

TrpR

CAP

GAL4

GR (glucocorticoid receptor)

(a) What is the small molecule that controls its’ activity?

(b) In the presence of this molecule, is the protein on the DNA or off the DNA?

(c) What is the consequence for transcription?

(2) Fill out the table with the words ON or OFF:

(3) Answer the three questions for each of the following enzymes:

Swi/Snf

HAT

HDAC

DNMT

(a) Describe the activity of each of the enzymes.

(b) What is the consequence of this enzyme activity?

(c) How does this enzyme know what genes to act on?

1. An isolated population of chimpanzees was discovered on an island. Biochemical studies show that 1 out of 9 chimpanzees in this population are homozygous for a mutation in the gene encoding the enzyme, hexokinase. This mutation completely inactivates the enzyme so that no activity is detected in the blood. A female who exhibits hexokinase activity mates with a male who is a carrier of the hexokinase mutation. What is the probability that their first baby chimpanzee will be homozygous for the hexokinase mutation? Assume that the population is in Hardy-Weinberg equilibrium.

2. Cystic fibrosis is an autosomal recessive, fully penetrant trait in humans.    A man who is unaffected by cystic fibrosis has two unaffected parents and a sister with cystic fibrosis.  The man plans to marry a healthy woman, who was adopted and has no knowledge about her biological parents except that they are both Caucasian. The frequency of cystic fibrosis in the Caucasian population is 1/2500. Assuming that this population is in Hardy-Weinberg equilibrium for the CF gene, what is the probability that this couple's first child will have cystic fibrosis?

14. How does Coronavirus infect the lung tissue?

A. The S-spike protein binds to Angiotensin Converting Enzyme 2 on Type 2 alveolar cells, leading to its destruction, a reduction in surfactant and Coronavirus replication .

B. The S-spike protein binds to Angiotensinogen on Type 2 alveolar cells, leading to its destruction, a reduction in surfactant and Coronavirus replication .

C. The S-spike protein binds to Angiotensin Converting Enzyme 2 on Type 1 alveolar cells, leading to its destruction, a reduction in surfactant and Coronavirus replication .

D. The S-spike protein binds to Angiotensinogen on Type 1 alveolar cells, leading to its destruction, a reduction in surfactant and Coronavirus replication .

17. Bacteria found in our colon has the following roles EXCEPT:

A. Metaboloze undigested food, causing Gas

B. Production of Vitamin K

C. Production of Bile

D. Microbial Antagonism

A mouse gene was identified and determined to be required for formation of heart muscle. A gene with a similar sequence was identified in the human genome. What experiment could scientists do to determine if the mouse and human genes have similar functions?

The scientist could place the normal human gene into normal mice and see if the resulting mice are viable.

The scientist could search the human genome for genes that encode proteins that are identical to the protein encoded by the mouse gene.

The scientist could place the normal human gene into mutant mice to see if heart muscle forms in the mouse.

The scientist could place the mutant mouse gene into humans to see if humans develop without heart muscle.

This week you learned about the evolution of human resource management, the importance of human resources to an organization, and some of the emerging issues facing human resource professionals today. Your goal is to convince the key decision makers at the company to hire you to work on a human resource project.

Your assignment this week is to prepare your credentials for the proposal. Include the following in your paper:

1-2 paragraphs outlining why you think human resource management is important.

1-2 paragraphs highlighting your accomplishments, the unique skills you bring to the project, and what makes you stand out from the other consultants applying to work on the project.

Code the game of Rock, Paper, Scissors between a human player and the computer. You can check out the game on Wikipedia if you are not familiar with it. Create a 4 option menu with the human player choices, plus the option of exiting the game. Randomly determine the computer’s choice (although a great deal of AI research has gone in to determining the best computer move).

• Loop the game until the human player exits.

• Count the number of times the computer and the human each won.

When you’re done, display the total number of hands played, the number of times the human won and the number of times the computer won. Declare the highest total of winning hands the game winner.

1. Evaluate the double integral for the function f(x,y) and the given region R.

R is the rectangle defined by -2  x  3 and 1   y  e⁴

2. Evaluate the double integral

for the function f(x, y) and the region R.

f(x, y) =

; R is bounded by the lines

x = 1, y = 0, and y = x.

3. Find the average value of the function f(x,y) over the plane region R.

f(x, y) = xy; R is the triangle bounded by y = x, y = 2 - x, and y = 0

4. Verify that y is a general solution of the differential equation and find a particular solution of the differential equation satisfying the initial condition.

y =

;  

= −2xy²;  y(0) = 7