put the following processes of protein synthesis translation steps in the correct order :

A- the next aa2-tRNA attach to the second codon on mRNA located in A site

B- then large ribosomal subunit binds the small ribosomal subunit forming an active ribosome

C- the ribosome breaks down and the mRNA dissociates and protein is released

D- the ribosome small subunit binding to the mRNA

F- the first amino acid moves to exit site and leaves the ribosome

G- ribosome moves on mRNA and by 1 codon this process is called translocation

H- the tRNA is charging with amino acid at 3' OH end

i - the aa1-tRNA carries the anticodon attaches directly with mRNA in the start codon located in P site

1-

2-

3-

4-

5-

6-

7-

8-

1.A single nucleotide mutation in the third position of a codon more often than in the second or first position

a) has no effect on the amino acid sequence of the encoded protein.

b) alters the reading frame of the mRNA.

c) changes an amino acid in the encoded protein.

d) introduces a stop codon into the mRNA.

2. The synthesis of new DNA requires the prior formation of RNA oligonucleotides to serve as primers. This latter observation is evidence in support of the hypothesized existence of

a) that Crick’s postulate of the flow of information is incorrect.

b) the endosymbiotic origin of mitochondria and chloroplasts.

c) an ancient RNA world

d) the abiotic synthesis of organic peptides

3.A frameshift mutation could not result from

a) a single nucleotide insertion

b) a dual nucleotide insertion

c) a single nucleotide substitution

d) a single nucleotide deletion

The article:

GLOBAL CARTELS REDUX: The Amino Acid Lysine Antitrust Litigation (1996) by John M. Connor

http://docplayer.net/amp/54191121-Global-cartels-redux-the-amino-acid-lysine-antitrust-litigation-1996-john-m-connor-1.html

Read the article, answer the question.

2. Give the history of lysine industry. a. What type of product is lysene? Where has its historical demand come from? How has this change over time? b. How did the early manufacturers of lysene price? How did technological change affect their pricing? c. How did ADM’s entry affect the market? d. How did a cartel emerge? How was it detected? What legal procedures occurred that ADM (and other firms) participated in? Which one of these is the article concerned with?

If I was culturing a bacteria in less than 20degC and raised the temperature to 37degC after a few hours, how will this temperature change affect the:

a) transition temperature of the lipid bilayer

b) activity of the enzyme membrane desaturase

In Enzyme kinetics can someone explain how color develops by explaining the chemical reaction that occurs with BAPNA.

also Draw the structure of benzamidine and comment on the type of inhibitor that benzamidine is and how this relates to its structure.

The data below were collected for an enzyme-catalyzed reaction.

[S] (mM)

0.25

0.50

1.00

2.00

V0 (mM*s-1)

2.00

3.33

5.00

6.67

Determine Vmax and Km for this reaction.

The physical structure of a protein often reflects and affects its function.

Discuss how the structure of a protein affects the function of TWO of the following.

a) Muscle contraction
b) Regulation of enzyme activity
c) Cell signaling

How is metal ion catalysis an effective catalytic strategy? Give two possible roles for a metal ion in enzyme catalysis and talk about how each role helps to lower the activation energy of the reaction.

What would change if the lungs did not produce Angiotensin-Converting Enzyme?

What is/are the differences between the two sphincters of the urinary bladder? (Can you make a chart for this one :) )

Thanks so much <3

What would be the predicted cutting frequency of a restriction enzyme that recognizes a 10-base recognition sequence?

a. 1048576

b. 10000

c. 0.00001

d. 4.0 E-10

e. 9.5 E-7