In: Biology
Enzymes are kinetically important for many of the complex reactions for plant and animal life to exist. However, only a time amount of any particular enzyme is required for these complex reactions to occur. Explain.
In: Biology
Devise a restriction analysis method to confirm the desired recombinant; use a single most appropriate enzyme. Calculate the expected sizes of the restriction fragments from each, the vector and the desired recombinant
In: Biology
Know all of the hormones that have their effects on the kidneys, and what those specific effects are, and know about the hormones and enzyme (Renin) released by the kidneys and their target tissues and effects at the various tissues.
In: Anatomy and Physiology
1. Please explain why “noncompetitive inhibition”- where it is observed experimentally- is more likely to be a coincidence than a reveal of something profound in the mechanism of an enzyme (in contrast to competitive and uncompetitive inhibition).
In: Chemistry
Ocean pout, a fish found in cold waters, produce an antifreeze protein that allows them to survive at near freezing temperatures. Your company plans to genetically engineer bacteria to grow antifreeze proteins to improve the storage of ice cream products
a) The vector and PCR product that you will use to clone the antifreeze gene are shown above along with the locations of three different restriction enzyme cut sites Which restriction enzyme (Hindill, BamH1, or EcoR1) will allow you to successfully clone this transgeno? Brietly justify your answer.
b) Describe how you will select for bacteria that carry the recombinant DNA molecule once you have completed the transformation
In: Biology
Experiment 3: Cloning a DNA Fragment into a Bacterially-Derived Plasmid Vector
Foregin DNA = 720 Plasmid DNA 2804
Post-Lab Questions
1. What is the expected size of the plasmid plus the cut foreign DNA?
2524 base pairs
2. What type of ends do the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning?
3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does this enzyme work?
4. How would you clone a gene into a plasmid if there were no common restriction sites between the two DNA sequences?
In: Biology
DIPF and Sarin and "organophosphate" insecticides are all irreversible inhibitors of enzymes with a "special" -- highly reactive -- active-site Ser residue. Which of the following is true about DIPF (or Sarin or organophosphate insecticides)?
| A.) | DIPF forms a covalent phosphoester bond to the side chain of a Ser residue in enzymes that DIPF inhibits. |
| B.) DIPF (or Sarin or organophosphate insecticides) cannot kill humans (or any vertebrate). |
| C.) DIPF inhibition is reversible. Hence, an EI complex is analogous to an ES complex. |
| D.)After DIPF reacts with an enzyme (to inhibit it) the phosphate part of DIPF is released and the rest of the molecule, including F, remains attached to the enzyme. |
| E.) none of the above is a true statement. |
In: Chemistry
In: Biology
1. Because plant and animal life on earth is impossible without photosynthesis
A. environmental pollution is not a problem
B. there is concern for increasing numbers of plant species that face extinction
C. worldwide decline of insect diversity is of no concern
D. there is no need to consider conservation biology
2. Reactions with a positive ΔG
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can occur as part of coupled reaction |
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are reactions that can never happen |
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reactions that do not require activation energy |
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are exergonic |
3. The induced fit is an improved model for enzyme function than the lock and key model because it considers
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considers the Gibbs free energy of the reaction |
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considers specificity of the enzyme |
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considers the catalytic activity |
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considers the rate of reaction |
In: Biology