You are a researcher at a small biotech company and your company has just obtained the license for use of a human GENOMIC DNA fragment putatively encoding a potentially novel protein, which is thought to regulate p53, the known tumor supressor protein. The scientists who originally cloned this GENE fragment HDM5 "claim" that HDM5 shares 90% DNA sequence homology with one of the HDM2 genes (refer to the review Levine & Oren, 2009). They propose that HDM5 may have HDM2-like properties and may be involved in regulating cell proliferation, and thus a good target to potentially develop as a cancer therapy. Your company has asked you to characterize the gene and gene products, as well as to provide an opinion as to its potential human therapeutic uses.

1. Before proceeding, you wish to know whether this gene is actually expressed in humans (your target market for therapeutic development). ?Describe, in detail, 2 efficient experimental approaches to answer this question. ?Discuss whether your proposed methods measure synthesis or accumulation of the product

3. (10pt) Your colleague professor Stu Dentgenerated a genome-wide DNA methylation map for normal colon cells using MRE-seq (a restriction enzyme approach) and MeDIP-seq(an immunoprecipitation approach). In an intergenic region, he found an interesting locus. This locus is about 20kb. On one end of the locus, there is a 2kb CpG rich stretch that has both relatively lowMRE-seq and MeDIP-seq signals. The rest 18kb has high level of MeDIP-seq signals. (A) (3pt) Why might you suspect that this region encodes for a novel gene? (B) (4pt) You decide to look at histone modification patterns across this regionfor more evidence. There are several genome-wide datasets available for this cell type: H3K4me1, H3K4me3, H3K27me3, H3K9me3, H3K36me3, and H3K9Ac. Which histone mark would youinvestigatefor this locus and why? (C) (3pt) Suggestat least one other source of data (NOT annotation or bisulfide sequencing) that may help you confirm or refute your suspicion, and why you think it may help?

Larry and Moe operate a large restaurant in a busy downtown area of a large city. For breakfast and lunch, they generally have a line of customers waiting. Fire codes prevent them from adding additional seating. The contribution format income statement for the most recent week follows:

Sales (4,960 meals served) $123,008

VC 63, 984

CM $59,024

FC 25,000

NI $34,024

Larry wants to automate food delivery. There would be no more servers. Orders would be placed electronically on iPads located at each table. Food would arrive via an overhead “railroad”. Larry believes that the cost of this system would be $11,200 per week and it would reduce variable costs by $3.00 per person. He thinks that maybe prices could be increased as customers will enjoy the novel environment.

1. Prepare a contribution format income statement using Larry’s predictions. Assume the price and the number of meals served stays the same.

2. Compute the price increase that would be required to achieve a target NI of $40,000 per week.

Mitchell MD, Chivers DP, Brown GE, Ferrari MCO. 2016. Living on the edge: How does environmental risk affect the behavioural and cognitive ecology of prey? Anim Behav. 115: 185-192. Greig EI and Webster MS. 2014. How do novel signals originate? The evolution of fairy-wren songs from predator to display contexts. Anim Behav. 88: 57-65. Petrů M, Špinka M, Charvátová V, Lhota S. 2009. Revisiting play elements and self-handicapping in play: A comparative ethogram of five Old World monkey species. J Comp Psychol. 123(3): 250-263.

1. What two studies examined proximate causes of behaviour? What evidence do the authors provide to support your answer?

2. What two studies examined ultimate causes of behaviour? What evidence do the authors provide to support your answer?

3. Which of the above (1 only) studies examines the evolution of a behaviour? What evidence do the authors provide to support your answer?

Suppose you wish to be outstanding in your field by studying plant cell biology. You discover a new strain of wild lima bean plants from Costa Rica that is resistant to being killed by a hydrophobic pesticide. Which one of the following states the most reasonable hypothesis that could explain the nature of the pesticide resistance?

A) Many chloroplast proteins are encoded in the plant nuclear genome. A nuclear gene encoding an enzyme involved in the dark reactions might confer resistance by degrading the pesticide.

B) In the resistant strain, the plant cell wall might contain a novel phospholipid that binds to the pesticide and prevents its entry into the plant cells.

C) The endoplasmic reticulum in the leaf cells of the resistant strain might contain a mutant version of a cytochrome P450 enzyme that helps to remove the pesticide from the membrane.

D) The resistant strain has mutations in actin that inhibit the development of pluripotent cells in the stem, re. This results in active transport of the pesticide via LDL particles interacting with coated pits on the mitochondrial thylakoid membranes.

E) None of the above

The world post Covid 19 has created the concept of social distancing which will impact on the way we do business. Online purchasing is expected to increase because it will reduce human interaction and contribute to the reduction of Covid 19 related illnesses. However, online purchases have created contemporary legal issues which may be a novel for most legal systems in several Caribbean Countries.

You are required to critically discuss the concept of online purchases and the various methods used in online transactions with supporting case laws.

10 marks

Secondly, critically discuss FIVE (5) CONTEMPORY CASE LAWS related to online purchasing at the International Level, focusing on the judgements handed down in those cases and most importantly how do you think that will impact on the Caribbean online purchaser.

20 marks

Finally, bearing in mind your answers above; what do you think (your opinion) that Caribbean Governments can initiate via its legal systems to enhance and protect online purchasers legal rights?

10 marks

1. In terms of word learning, what is fast mapping?

2. Which of the following is NOT true regarding co-sleeping?​

3. A classic experiment using the three mountains task with 5- and 6-year-old children shows that they

Subject- ( App Development for Web) ( language C#, software -visual studio)

I have created a 'Calculator' named program in visual studio. This is the main console program(Program.cs) shown below. Then i have created a "CaculatorLibrary" named project by adding the project from FILE--->ADD--->NEW PROJECT. Then i selected library and selected classLibrary(.NETcore). The programs for both are given below. There are no errors in the program. Now the requirement is to build unit test project within this project that will perform atleast 10 tests.The tests must be like add rwo positive numbers,multiply two positive numbers and so on ... . This test project will also be created by FILE--->ADD--->NEW PROJECT.But here instead of library select test .

Program.cs
using CalculatorLibrary;
using System;

namespace CalculatorProgram
{
  

class Program
{
static void Main(string[] args)
{
bool endApp = false;
// Display title as the C# console calculator app.
Console.WriteLine("Console CalculatorProgram in C#\r");
Console.WriteLine("------------------------\n");
Calculator Calculator = new Calculator();
while (!endApp)
{
// Declare variables and set to empty.
string numInput1 = "";
string numInput2 = "";
double result = 0;

// Ask the user to type the first number.
Console.Write("Type a number, and then press Enter: ");
numInput1 = Console.ReadLine();

double cleanNum1 = 0;
while (!double.TryParse(numInput1, out cleanNum1))
{
Console.Write("This is not valid input. Please enter an integer value: ");
numInput1 = Console.ReadLine();
}

// Ask the user to type the second number.
Console.Write("Type another number, and then press Enter: ");
numInput2 = Console.ReadLine();

double cleanNum2 = 0;
while (!double.TryParse(numInput2, out cleanNum2))
{
Console.Write("This is not valid input. Please enter an integer value: ");
numInput2 = Console.ReadLine();
}

// Ask the user to choose an operator.
Console.WriteLine("Choose an operator from the following list:");
Console.WriteLine("\ta - Add");
Console.WriteLine("\ts - Subtract");
Console.WriteLine("\tm - Multiply");
Console.WriteLine("\td - Divide");
Console.Write("Your option? ");

string op = Console.ReadLine();

try
{
result = Calculator.DoOperation(cleanNum1, cleanNum2, op);
if (double.IsNaN(result))
{
Console.WriteLine("This operation will result in a mathematical error.\n");
}
else Console.WriteLine("Your result: {0:0.##}\n", result);
}
catch (Exception e)
{
Console.WriteLine("Oh no! An exception occurred trying to do the math.\n - Details: " + e.Message);
}

Console.WriteLine("------------------------\n");

// Wait for the user to respond before closing.
Console.Write("Press 'n' and Enter to close the app, or press any other key and Enter to continue: ");
if (Console.ReadLine() == "n") endApp = true;

Console.WriteLine("\n"); // Friendly linespacing.
}
return;
}
}
}

CalculatorLibrary.cs

using System.Diagnostics;
using System;
using System.IO;

namespace CalculatorLibrary
{
public class Calculator
{
public Calculator()
{
StreamWriter logFile = File.CreateText("calculator.log");
Trace.Listeners.Add(new TextWriterTraceListener(logFile));
Trace.AutoFlush = true;
Trace.WriteLine("Starting Calculator Log");
Trace.WriteLine(String.Format("Started {0}", System.DateTime.Now.ToString()));
}
public double DoOperation(double num1, double num2, string op)
{
double result = double.NaN; // Default value is "not-a-number" which we use if an operation, such as division, could result in an error.

// Use a switch statement to do the math.
switch (op)
{
case "a":
result = num1 + num2;
Trace.WriteLine(String.Format("{0} + {1} = {2}", num1, num2, result));
break;
case "s":
result = num1 - num2;
Trace.WriteLine(String.Format("{0} - {1} = {2}", num1, num2, result));
break;
case "m":
result = num1 * num2;
Trace.WriteLine(String.Format("{0} * {1} = {2}", num1, num2, result));
break;
case "d":
// Ask the user to enter a non-zero divisor.
if (num2 != 0)
{
result = num1 / num2;
Trace.WriteLine(String.Format("{0} / {1} = {2}", num1, num2, result));
}
break;
// Return text for an incorrect option entry.
default:
break;
}
return result;
}
  
}
}

Scenario

Mary Ann, a thirty-two-year-old woman, lives in Clinton, Connecticut with her son, Tony, who is eight-years-old, and her boyfriend Jack. Mary Ann also takes care of her mother, Elma, who is elderly and lives with them in their home. Because Jack has trouble holding a job and rarely is employed for more than a few weeks at a time, Mary Ann works as a waitress during the day and as a bartender at night. It is common for her to be gone to work from 5:00 am to midnight. When Mary Ann is home, Jack is generally a decent fellow. He helps around the house, runs the occasional errand and watches a lot of TV.

However, when Mary Ann is at work, Jack behaves quite differently. He drinks most of the time and sits in front of the TV watching baseball. He doesn't care much for Tony or Elma, and he refuses to cook them food so they eat what they can find around the house. As a result, both are very thin and slightly malnourished. When Jack drinks a little too much and his favorite baseball team loses, he gets very angry. Dealing with an eight-year-old boy is the last thing he wants to do, so he sometimes locks Tony in the hall closet to punish him for misbehaving. On a several occasions, Jack has hit Tony when he is angry and Tony regularly has bruises on his arms and legs from the rough way that Jack handles him. Mary Ann notices the bruises, but Jack always explains that Tony is a boy and boys play rough, so the bruises are just from sports or kidding around. On a few occasions, Jack has also hit Elma, but he explains these marks to Mary Ann by saying that Elma is just old and ran into a wall or tripped and fell. He also regularly steals money from Elma’s purse, but Elma can never remember how much money she had so she never tells Mary Ann.

            Tony’s teacher, Miss McKay, has her suspicions that Tony is being abused, but Tony denies that there are any problems at home because he is afraid that his mom will get into trouble. Although Miss McKay is concerned, she decides that Tony is a good kid and she will believe him that there are no problems at home. When Tony is sent to the school nurse because he seems to have gotten very thin, the nurse gives him a cookie and tells him to eat all his meals like a good boy, then sends him back to class with a smile.

            One day, Elma is walking Tony to school, when she trips on a curb and falls down. A neighbor hurries out to help her up, and he notices the bruises on both Elma and Tony. He also notices that they both seem thin and pale. The neighbor asks Elma if everything is okay and Elma tells him about Jack. The neighbor then calls the police and reports the situation.  

Questions  

1) a) Identify any abusive situations in this home.

b) What type of abuse did occur or is occurring?

c) Who is responsible for the abuse?

2) a) Identify who, if anyone, had a responsibility to report an abusive situation in this case.

b) Did the individual(s) you identified in “2 a” meet that responsibility?

c) What information needed to be reported?

d) To whom should this information have been reported?