1.You have discovered a novel toxin-encoding gene in a bacterial pathogen. An adjacent gene encodes a putative transcriptional regulator protein. You decide to identify the promoter for the toxin gene and investigate whether the transcriptional regulator protein controls expression from this promoter. Describe your experimental approach to investigate these questions

2a.Describe how the lac repressor protein is involved in controlling expression of the lac operon.

2b.Explain the difference between homologous recombination and site-specific recombination.

3a.Most genome sequencing projects have used an approach known as "shotgun genome sequencing”. Briefly describe how shotgun sequencing works.

3b.DNA transposons and retrotransposons are both types of mobile elements. Describe these two mobile elements and explain how they differ from each other.

4a.For the following restriction enzymes: A) Xmal C|CCGGG (B) Smal CCC|GGG (C) Sdal CCTGCA|GG (D) Pstl CTGCA|G

(i) What is the length and nature (5’ or 3’) of the overhang generated by each restriction enzyme? (ii) Which pair of these enzymes generate sticky ends that are compatible with each other?

4b.The successful construction and transplantation into a cell of a synthetic Mycoplasma genome was an important milestone in synthetic biology. Briefly describe the approach used in this project

5a.What was different between the genome sequencing approaches used by the public human genome project and the private human genome project led by Craig Venter?

5b.What is systems biology? Give two examples of experimental approaches that have been used in systems biology research.

5c.Describe the principles behind and the applications of the following:

a) Reverse transcriptase-PCR b) Cloning DNA into a plasmid vector c) SDS-PAGE d) Restriction mapping e) Sanger Sequencing of DNA

Barney’s Bookstore

Barney’s Bookstore plans to order a popular new hardback novel. It can purchase any number of this book from the publisher, but the unit cost of the book depends on the number ordered as shown below:

Order Qty Cost/Unit

1000+ $15

2000+ $14

3000+ $13

4000+ $12

Barney’s believes that demand for the book will depend on price. The lower the price, the higher the demand. Based on past data it estimates the following demand:

Price Demand

$30 2000

$28 2400

$26 3000

$24 3800

$22 4800

Also, as with most hardback novels, this one will eventually come out in paperback. Therefore, if Barney’s has any hardbacks left when the paperback comes out, it will put them on sale for $8, at which price it believes all leftovers will be sold.   In this situation Barney’s has to decide on both the price to charge and the quantity to order from the publisher.

Build an Excel model to calculate total profit for any given price and order quantity.

In the above model create a 2-way data table of price versus order quantity. Price should vary as shown in the price-demand table. Order quantity should be the 5 values shown in the price-demand table. This table should give you total profit for any combination of price and order quantity.

Note: Use good structure and formatting in all your Excel models. Don’t display excessive number of decimal places. Anyone reading your Excel model should easily be able to figure out what you are doing.

1.The novel coronavirus, SARS-Cov2, which is responsible for the disease Covid-19 is a positive strand RNA ((+)ssRNA) virus. From what you learned in BIOL1020, suggest a model of how the SARS-Cov2 genome is replicated. The key enzyme for viral replication is unique to the virus and not found in the human host. What is the name of the enzyme, what is its enzymatic function, and how would you explain that a drug called Remdesivir (an adenosine analogue that causes premature termination of RNA synthesis) is currently the most promising drug against a range of RNA viruses, including Ebola ((-)ssRNA virus), MERS ( (+)ssRNA virus ) and SARS-Cov2 ((+)ssRNA virus)?

2.A mutant E. coli strain is found with a mutation affecting some of its tRNA(Cys). The wild type normally produces a tRNA that recognizes the codon 5’ UGC 3’, and is charged with the amino acid Cysteine (Cys) (its notation is tRNA(Cys)). The mutant tRNA is still charged with Cysteine, but the mutation is in its anticodon that now has the sequence 5’- UCA-3’. How will some of the proteins produced in these E. coli cells be different from the proteins produced in the wild type cells?

Your summer research project requires that you purify a novel protein expressed in bacteria. After centrifugation of your cell lysate, you find that there are 4 major proteins left in the supernatant fraction that have the following pI values: protein A = 8.0, protein B = 4.0, protein C = 6.8, and protein D = 5.3. If these proteins are passed over a cation exchange column in a solution buffered to pH 5.2, what will be the order in which they will pass through the column (from first to last to elute) if a linear gradient of increasing salt is applied? Why?

A, B, C, D

C, A, B, D

B, D, C, A

D, B, C, A

D, B, A, C

b) In John Grisham’s novel, “A Painted House”, the 7-year old Luke has the following thoughts: ..”If I told him about Hank, I knew exactly what would happen. He’d march me down the front yard and we’d have a confrontation… Hank would misjudge his opponent, and before long the stick would come into play….And we’d be left short-handed. I’d be expected to pick even more cotton. So I didn’t say a word.” Luke understands the need to choose the best strategy (in our class context, Game Theory), since he’d like to avoid doing more arduous labor than would otherwise be required of him. As a young child, you probably went through a similar train of thought that led you not to do something that would have been the morally correct thing to do. Give one example.

250 words required

A publisher for a promising new novel figures fixed costs​ (overhead, advances,​ promotion, copy​ editing, typesetting, and so​ on) at $51,000​, and variable costs​ (printing, paper,​ binding, shipping) at ​$1.70 for each book produced. With this​ pricing, 4147 books need to be produced and sold at $ 14.00 each for the publisher to break even. ​ However, rising prices for paper require an increase in variable costs to ​$2.20 for each book produced.

Use this information to complete parts a. through c.

a.  What strategies might the company use to deal with this increase in​ costs? Choose all that are reasonable.

A. Find different suppliers to try and lower the variable costs.

B. Increase the selling price of the book.

C. Decrease the fixed costs.

D. Increase the font size of the print in the book.

b.  If the company continues to sell books at ​$14, how many books must they now sell to make a​ profit?The publisher must produce and sell at least ___ books to make a profit.

​(Round up to the nearest whole​ book.)

Engaging in regular exercise has both psychological and physical benefits. You believe you have a novel way to help obese adults start and maintain a regular exercise program. Your idea is to have them visualizing themselves engaging in exercise (walking) via virtual reality for two weeks before having them actually go out and walk. From data compiled by the National Institutes of Health, you know that obese adults in the United States walk, on average, 0.40 miles per day, with a standard deviation equal to 0.16 miles, and that the distance walked per day by obese adults is normally distributed. You obtain a sample of 49 obese adults for your study, apply the exercise program, and determine that they walked, on average, 0.45 miles per day following the virtual reality intervention. You want to assess whether this intervention has been successful. So to get started on this study…

1. State a set of appropriate hypotheses for this study using statistical language and notation.

2. Are your hypotheses directional or non-directional?

3. Is your statistical test going to be a one-tailed or two-tailed test?

4. State an appropriate alpha level.

Case study: The recent outbreak of novel coronavirus (COVID-19) has introduced new challenges to the business environment. It is also having an impact on the global economy with tourism, aviation, education, and hospitality the initially hardest-hit industries. Almost all global supply-chains are affected at some level. Realistically, many sectors will be affected to different degrees, with many organizations implementing policies to limit employee travel and to prepare employees to work from home if necessary and if possible, to ensure the safety of their employees. The outbreak is moving quickly, and most countries are trying to respond quickly to contain the impact. However, the spread of the virus may continue through 2020 and impact the operations of many industries for months to come.

taking any university as an example

Q. Critically analyze the required types of change to minimize the impact of the COVID 19 virus on the university. Then analyze which images of change management could be adopted by the change agent to manage such a situation. (maximum 800 words )

Please search online business press and find a recent article related to “Accrual versus Cash Accounting,” “Revenue Recognition,” or “Accounting Fraud.” Then please provide the webpage link of the article together with the answers to the following questions.

Question 1: How is your article of choice related to topics above and why you think it is interesting?

Question 2: What are the practical implications of this article?

In an essay, define and describe the federal-default exchange program and indicate if any of the states listed in Exhibit 22.1on page 395 have become or anticipate becoming fully self sustaining by the end of 2015 as dictated in the Affordable Care Act (ACA).

Please use the reference below for exhibit 22.1

Morrisey, M. A. (2014). Health insurance. Chicago, IL: Health Administration Press.