The enzymes involved in glycolysis are.....

• A.

  constitutive proteins

• B.

  inducible proteins

• C.

  repressed proteins

• D.

  regulated proteins

Briefly describe how protein and lipid metabolism are linked to glycolysis and the Krebs cycle, and determine the ATP yield of each.

1. NADH and FADH2 deliver their high energy electrons to

A. glycolysis

b. the electron transport chain

c. oxygen

2. In fermentation, which set of pathways is running, or operating?

A. glycolysis

b. transition

c. Kreb's cycle (also called TCA cycle or citric acid cycle)

d. electron transport chain

e. all of the above

3. As electrons are passed down the electron transport chain, the energy is used to pump ______ across the membrane.

A. B.H+, or hydrogen ions

b. NADH

c. H20

For BIOCHEM

1. When we refer to "anabolic" biochemical reactions, what do we mean? Give an example of an anabolic reaction. (You can describe in words, you dont need to show a balanced reaction).

2. Stage 1 of metabolism refers to the beginning of deigestion of carbohydrates, fats and proteins. Where do proteins begin stage 1 digestion? What happens at the beginning of Stage 1 digestion of proteins?

3. Which coenzymes serves as an oxidizing agent in glycolysis?

4. Write the net reaction for glycolysis.

What is the numbering scheme for naming fatty acids and what is the difference between cis and trans fatty acids?

What are the general properities of glycosidic linkages?

What would happen if we cannot process an amino acid that is needed by our body? Can you describe a disease associated with defects in the amino acid biosynthetic pathway?

Could the potato enzyme extract prepared in this exercise (phenoloxidase) be used as a source of another type of plant enzyme such as peroxidase? Why or why not?

Why are mycobacteria (Mycobacterium)harder to control than other bacteria?

What makes an enzyme specific(what causes an enzyme to be specific)?

Regarding the LDH purification lab, what characteristics of a protein/enzyme allow us to quantify total protein versus enzyme activity?

In the Anfinsen protein folding experiment the sequence of removing urea or BME makes a difference for functional refolding of the protein. Explain why removing BME first leads to a nonfunctional enzyme, but removing urea first leads to a functional enzyme. Why does adding small amounts of BME alone to the solution with the nonfunctional enzyme "rescue" the function

biochemistry