5. In cattle, polled (absence of horns) expresses complete dominance over horned and roan is the result of the heterozygous condition of the genes for red and white coat color. (Red and white alleles express co-dominance) Assume both traits are on separate pairs of autosomes.

a) What proportion of the offspring of a roan heterozygous polled bull and a roan horned cow would be predicted to be roan and horned?

Capillary number is proportional to { (viscous force) / (surface tension force) } and is used in momentum transfer in general and atomization and 2-phase flow in beds of solids calculations in particular. It is equivalent to (We/Re).

___________ Which is the friction factor for ReD = 600 and e/D = 0.002 in pipe flow?

___________ What is the friction factor for ReD= 3 x 105 and D/e = 5000 in pipe flow?

There is a group of kindergarten children observed who have two different shirt colors, red and blue. Red = 1 and Blue = 0. The sample size of 8 can be formed as 1 1 0 0 0 0 1 0. What are the lower and upper bounds of the 95 percent confidence interval for this sample if we believe that the sample is drawn from a binomial distribution?

Conditional Probability Problem: An urn contains 5 red balls, 4 green balls, and 4 yellow balls for a total of 13 balls. If 5 balls are randomly selected without replacement what is the probability of selecting at least two red balls given that at least one yellow ball is selected?

a) 0.59

b) 0.61

c) 0.63

d) 0.65

e) 0.67

1. What are necessary buffer components?

2. Write reaction equations to explain how your acetic acid/sodium acetate buffer reacts with an acid and a base. Why pH does not change? Explain.

3. At which point does a buffer lose its effectiveness? Use your data to determine the buffer capacity of Buffer A and Buffer B. Explain.

1.What is management's responsibilities for reporting internal control under Section 404 Sarbanes Oxley Act ?

2.What is auditor's responsibilities for reporting internal control under Section 404 Sarbanes Oxley Act?

1. What (if anything) is wrong with the following code:

   int redCount = 0, blueCount = 0; 
   Scanner scan = new Scanner(System.in); 
   System.out.println("Please make a selection: ");
   String option = scan.next(); 
   
   while(!option.startsWith("q") || !option.startsWith("Q"))
   {
       if(option.equals("red"))
       {
            redCount++;
       }
       else
       {
            blueCount++;
       }
       System.out.println("Please enter another value: ");
       option = scan.next(); 
   }
   System.out.println("Red: " + redCount + ", Blue: " + blueCount); 

   		The condition is not a boolean expression.
   		A variable is out of scope.
   		There is nothing wrong with this code.
   		It will result in an infinte loop.
   		It contains a logic error that will cause it to function incorrectly.

2. What (if anything) is wrong with the following code:

   int redCount = 0, int blueCount = 0; 
   Scanner scan = new Scanner(System.in); 
   System.out.println("Please make a selection: ");
   String option = scan.next(); 
   
   while(!option.toLowerCase().startsWith("q"))
   {
       if(option.equals("red"))
       {
            redCount += 1;
       }
       else
       {
            blueCount += 1;
       }
   
   

       System.out.println("Please enter another value: ");
       option = scan.next(); 

   }
   System.out.println("Red: " + redCount + ", Blue: " + blueCount); 

   		A variable is out of scope.
   		The condition is not a boolean expression.
   		It will result in an infinte loop.
   		There is nothing wrong with this code.
   		It contains a logic error that will cause it to function incorrectly.

Have a standard curve and determine the concentration of the samples. Get the mean, standard deviation for the two samples tested in triplicate and run a t.test. Is there a difference between the two samples? These were two different sets of serum (two different pigs). I would think they will be different but let's see.

Questions

As you should recall, glucose is a reducing sugar. There are many chemical assays for reducing sugars such as using Nelson's alkaline copper reagent or 3,5 dinitrosalicylic acid (DNS). Why is an enzymatic assay the preferred method for blood glucose?

Convert a typical measurement of 95 mg/dL blood glucose into a mM amount.

The concentration of glucose inside a typical cell is about 1.0 mM. Why is the concentration of glucose inside the cell less than in the blood?

A typical red blood cell has a diameter of about 7 m. Although they are disk-shaped, let's assume they are a sphere and determine the number of glucose molecules in the cell?

Red blood cells are one of the few tissues that rely solely on glycolysis. How much ATP can be made from the amount of glucose in the red blood cell?

Based on questions 2-5, why is it important that the blood glucose concentration remain constant?

Maltose is a disaccharide of glucose (two glucose molecules linked by an alpha 1-4 glycosidic bond). A solution of maltose is tested with the DNS-reagent reducing sugar assay and found to be 50 mM. What would the concentration be measured as if this maltose solution is tested with the glucose-oxidase assay? Explain.

Search the literature, web-sites, wikipedia etc. and find an example of a clinical assay that uses coupled reactions involving an oxidase/peroxidase system. Reference you source and write down the reaction sequence.

Background: Determination of blood glucose is a fundamental test performed in a clinical laboratory. Normal fasting blood glucose levels range from 70-99 mg/dL in healthy adults and hyperglycemia is of course an indication of diabetes mellitus or other medical conditions. One of the more common methods for this determination is to use a glucose oxidase/peroxidase system.
The assay that will be used is called an Endpoint Enzymatic Spectrophotometric assay . Glucose oxidase (GOX) is found in various insects and fungi where it is used as an anti-bacterial agent [4]. GOX oxidizes -D-glucose in into D-gluconolactone with the subsequent production of hydrogen peroxide (eq. 1). Hydrogen peroxide is potent oxidizing agent used by many types of cells to kill pathogens. In the glucose assay the hydrogen peroxide that is released, combined with horseradish peroxidase (HPR), is used to oxidize a dye molecule that is monitored spectrophotometrically (eq. 2).
Many dyes have been used for this assay, but today, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS, figure 1) will be used since it is stable, readily soluble in water and non-toxc

data given
standards 0.726 0.479 0.235 0.139 0.094 0.037 blank
sample 1 0.509 0.48 0.454
sample 2 0.462 0.497 0.539


mg/dL
200
100
50
25
12.5