This is a question about the SDS - PAGE method, one of the protein analysis methods.

1. I would like to know the functions of resolving gel and stacking gel in SDS - PAGE analysis.

2. I would like to know the various reagents used in manufacturing resolving gel and stacking gel in SDS - PAGE analysis and their role. ( Please tell us in detail the role of each reagent. )

We would appreciate your detailed explanation of each question. 

(Please Typing)

The current price of a non-dividend paying stock is $35. Use a two-step tree to value an American put option on the stock with a strike price of $33 that expires in 12 months. Each step is 6 months, the risk free rate is 6% per annum (continuously compounding), and the volatility is 15%. What is the option price? Show work in detail and use a tree diagram (Use 4 decimal places).

Festive Floral is gearing up for online carnation sales this Mother’s Day. They specialize in selling organic extra-large red and pink carnations. The price is $0.7 per stem, which includes free one-day shipping to the customer.

Festive Floral pre-orders carnations directly from a certified organic farm at the cost of $0.15 per stem. The order is made prior to the growing season and flowers are grown accordingly, so the pre-order quantity cannot be changed. However, if Festive Floral runs out of pre-ordered carnations, they can always pick up more immediately from a local wholesaler that carries carnations of the same quality, at the price of $0.5 per stem.

(a) (5 points) Using exponential smoothing with parameter ?? = 0.9, what is your forecast for red carnations in 2018? What is the MAD of your forecast?

(b) (3 points) Based on the forecast above, what is the optimal number of red carnations to pre-order from the farm?

(c) (8 points) Repeat Parts (a) and (b) for pink carnations.

The carnations are sold in dozens, and each dozen comes with a designer gift tag that says “Happy Mother’s Day 2018 from Festive Floral!” The gift tags are identical for carnations of both colors.

(d) (5 points) Using exponential smoothing with parameter ?? = 0.9, what is your forecast for gift tags? What is the MAD of your forecast?

(e) (5 points) Using the same z-value as for carnations, what is the optimal number of gift tags to prepare? Is this more or less than the total dozens of carnations, and why?

(f) (4 points) Experiment with the exponential smoothing parameter ?? for the forecast of gift tags in part (d). What is the value of ?? that minimizes the MAD?

1. Which concept would you use to explain why investment spending did not grow strongly between 2011 and 2014 although interest rates were very low? Think about what drives investment spending and use a certain principle we covered in the chapter titled [Income and Expenditure] ( Around150 words)

Mike and Andy is a clothing store for teenagers. It is chronically short of cash, so arranges a 120-day commercial bill (BAB). According to the theory of management of assets, what use should it put this money to?

After thinking about how it could be done, explain the processes an entity might use to collect its debts.

Working parents with children see an increase in the price of babysitting. While they try to reduce their use of child care, they find that they have less money to spend on other goods with their budget after price increases. Explain what this says about the parents price elasticity of demand for babysitting. use charts to illustrate you answer.

Which concept would you use to explain why investment spending did not grow strongly between 2011 and 2014 although interest rates were very low? Think about what drives investment spending and use a certain principle we covered in the chapter titled [Income and Expenditure] ( Around150 words)

QUESTION 2

The following transactions were undertaken by ABC Pty Ltd during the financial year ended 30 June 2019.

1. Sold for $12,000 used machinery with a carrying amount of $75,000.
2. Issued ordinary shares for cash, $300,000.
3. Purchased land to be held for future expansion for $220,000.
4. Paid off a long-term $90,000 loan plus interest of $8,000.
5. Purchased machinery, giving $50,000 cash and signing a mortgage loan for $90,000.
6. Purchased shares in A Ltd to be held as an investment for $100,000 cash.
7. Sold a long-term government bond, with a carrying amount of $45,000, for $56,000, including $5,000 accrued interest.
8. Purchased shares in Rouge Ltd to be held as a long-term investment, paying $170,000 cash.
9. Issued 7% debentures for $500,000
10. Paid cash dividends of $60,000

Required:

1. Prepare the net cash flow used in investing activities section of the statement of cash flows. Must show your calculations
2. Prepare the net cash flow used in financing activities section on the statement of cash flows. Must show your calculations
3. What are the limitations of a statement of cash flows? Explain. You must disclose the source of your information by referencing and support your statements by readings and research.

Many medical professionals believe that eating too much red meat increases the risk of heart disease and cancer. Suppose you would like to conduct a survey to determine the annual consumption of beef by a typical American and want to use four pounds as the desired margin of error for a confidence interval of the population mean. Assume the population standard deviation is 20 pounds. Determine the sample size when:

a)A 90% confidence interval is desired for the mean amount of beef consumed.

b)A 99% confidence interval is desired for the mean amount of beef consumed.

Suppose you want to develop a confidence interval for the proportion of Americans who over-consume red meat with a desire margin of error of 0.1. Determine the sample size needed when:

     c)   A 90% confidence interval is desired for the proportion of Americans who over-consume red meat.

     d)   A 95% confidence interval is desired for the proportion of Americans who over-consume red meat.

Many medical professionals believe that eating too much red meat increases the risk of heart disease and cancer. Suppose you would like to conduct a survey to determine the annual consumption of beef by a typical American and want to use four pounds as the desired margin of error for a confidence interval of the population mean. Assume the population standard deviation is 20 pounds. Determine the sample size when:

a)A 90% confidence interval is desired for the mean amount of beef consumed.

b)A 99% confidence interval is desired for the mean amount of beef consumed.

Suppose you want to develop a confidence interval for the proportion of Americans who over-consume red meat with a desire margin of error of 0.1. Determine the sample size needed when:

     c)   A 90% confidence interval is desired for the proportion of Americans who over-consume red meat.

     d)   A 95% confidence interval is desired for the proportion of Americans who over-consume red meat