Students collected Beta-fructosidase enzyme activity data in the presence and absence of an unknown inhibitor. P-nitrophenol was used as a read out for enzyme activity. Quantity of product was determined from the absorbance of each sample after 5 minutes of reaction. Using the information, complete the table below and create a properly labeled Lineweaver burke plot with the appropriate axes and best fit lines for the data, in excel (graph is worth 6pts). Using this plot, calculate the approximate Vmax and Km values for the reactions with and without the inhibitor. A picture of your graph can be inserted into this document as a picture.

Normal Reaction

            Vmax: _________________ (1pt)

            Km:     _________________(1pt)

Inhibited Reaction

            Vmax: _________________(1pt)

            Km:     _________________(1pt)

What type of inhibitor is this? Be sure to justify your answer by discussing the Km AND Vmax values you calculated (2pts).

The production of a nationally marketed detergent results in certain workers receiving prolonged exposures to a Bacillus subtilis enzyme. Nineteen workers were tested to determine the effects of those exposures, if any, on various respiratory functions. One such function, airflow rate, is measured by computing the ratio of a person’s forced expiratory volume (FEV) to his or her vital capacity (VC). (Vital capacity is the maximum volume of air a person can exhale after taking as deep a breath as possible; FEV is the maximum volume of air a person exhale in one second.) In persons with no lung dysfunction, the “norm” for FEV/VC ratios is 0.80. Assume that the FEV/VC ratios are known to be normally distributed. For the 19 workers in the study, the mean FEV/VC ratio was 0.766 with standard deviation 0.0859. a. Based on your result, is it believable that exposure to Bacillus subtilis enzyme has no effect on the FEV/VC ratio? Please compute the p-value for the test in two different ways: (i) assuming σ = 0.09 and (ii) assuming σ is unknown. b. Based on this data, is it believable that σ = 0.09 ? Conduct a hypothesis test to answer this question.

For the following procedure, write a balanced chemical equation for each reaction. Make sure you include the state of matter (s,aq,g,l) and the type of reaction (decomposition, synthesis, single displacement, double displacement, combustion).

Reaction 1
a. Dip one end of a Q-tip in CONCENTRATED hydrochloric acid.
b. Dip one end of a second Q-tip in CONCENTRAED ammonium hydroxide solution.
c. Record initial observations. Caution: Do not observe reaction close to your eyes.
d. Bring the Q-tips close to one another BUT do not let them touch.
e. After you record your observations, flush with water, wrap in a paper towel, and throw in the trash can.

Reaction 2
a. Place a small piece of potato to a small beaker. PLEASE DO NOT WRITE “POTATO” AS A
REACTANT! There is an enzyme in the potato that acts as a catalyst – do research at home to
determine what this enzyme is!
b. Cover the potato with 3% hydrogen peroxide in the beaker.
c. Clean up your station – decant the solution and throw solid contents of beaker into garbage can,
rinse the beaker. Be sure lab table is clean.

Doctor Amanda Abraham is an expert cancer biologist. She received a 50 mg of newly synthesised chemical compound from an international collaborator to test its in vitro anticancer activity. Collaborator provided following information about the compound:

Physical state: odourless white crystalline solid Molecular weight: 500 Da
Solubility: very little solubility in water , but the compound is highly soluble in Dimethyl Sulfoxide ( DMSO )

Please help Amanda in advising on following 7 aspects:

1. Suggest an assay to be used for assessment of cytotoxicity of compound and provide reason for this selection.
2. What cells/cell lines should be used?
3. What experimental controls should be used and why?
4. What treatments doses should be tested and why?
5. What exposure times should be tested and why?
6. How would you know if the compound possess any anticancer activity or not?
7. Please outline the proposed assay plan for vitro cytotoxicity/anticancer activity.

The marathon is a 26.2-mile-long, endurance running race. Successful marathon runners will benefit from having leg muscles with __________.

i. fibers with a high concentration of myoglobin

ii. fibers depending on glycolysis for generation of ATP

iii. fibers with relatively low mitochondria content

Which of the following is a correct pair telling WHERE and HOW sodium ions are reabsorbed in the loop of Henle?

How does the motor unit recruitment pattern described in explain the increase in blood lactate which occurs above 50-60% Vo2max? Blood lactate is the buffered form of lactic acid derived from cellular anaerobic glycolysis. (HINT: connect motor unit recruitment order with collective metabolism. And high/low intensity training

My answer so far: The motor unit recruitment pattern described in the lecture materials tells us that when there is low intensity activity (rest to about 50% of Vo2max), minute ventilation (VE) parallels Vo2. We also learn that onset blood lactate accumulation (OBLA) occurs at about 66% Vo2max, and occurs between 50% and 80% Vo2max.

Use the demand-supply model to explain market outcomes.

Is there currently a surplus or shortage of human organs available for transplant? Using the demand/supply framework, explain how legalizing the trade for human organs will help move the market towards an equilibrium.

select a human-caused environmental issue (for example, climate change, species extinction, etc.) and research it further. Find a reliable, useful website that discusses the human impacts to the environment caused by this issuE.

full paragraph summary (150 words minimum)

Describe the concept of human rights . In your answer refer to the foundation document developed by the united nations that outlines our basic rights and freedom

identify three ways in which a client in age care could have their human rights ignored