: Sanger sequencing is the method of dideoxy sequencing that we use in Bio 2450. It is a modified PCR reaction that has the dNTPS replaced with a mixture of dNTPs:ddNTPs. In this section, go into detail on how the addition of ddNTP alters the produced PCR fragments and describe how we ultimately get the sequence.

In your own words, in detail explain the purpose of flexible budgets and the flexible budget performance report. Additionally, explain in detail what the purpose of computing the materials, labor and overhead cost variances.

a) Sketch and describe in detail a simple single pass composite boiler.

b) Explain its operation and state two advantages.

c) Sketch a biological sewage treatment plant, label it and explain its operation.

d) State five conditions under which oily water can be discharged overboard

You have one protein to mutate one specific residue to another. For example, the residue 50 of glutamate is designed to be substituted with alanine. What kind mutation technique will you use for this purpose. Please explain your chosen mutation method in detail.

Explain how to build a Short Straddle, what are the purposes of a Short Straddle strategy? Build a real life Straddle for a stock of your choice, pull the options contracts and paste them on the answer. Please explain each part of it, what the credit or debit will be for the transaction, include every detail of each option contract you will use to build the Straddle trade.

Chapter 9, Section 1, Exercise 005

Computer output for fitting a simple linear model is given below. State the value of the sample slope for the given model.

In testing if the slope in the population is different from zero, identify the p-value and use it (and a 5 % significance level) to make a clear conclusion about the effectiveness of the model.

The regression equation is Y ⁢=87.0 -6.9 X

. Predictor Coef SE Coef T P

Constant 87.013 4.415 19.71 0.000

X -6.9023 0.8054 -8.57 0.000

what is the sample slope?

what is the p- value?

is the model effective?

Patterns and structure of revenue for state and local government are important policy concerns because they establish who will pay for public services. Revenue revision can begin only with a clear understanding of where revenue policy leaves the state and its localities now and what available options have not been selected. Furthermore, it is useful to understand what conditions are like in surrounding areas. Evidence for such discussions can be drawn from sources such as the Department of Commerce’s Survey of Current Business (monthly); the Bureau of Economic Analysis; the Census Bureau’s Census of Governments (quinquennially), Governmental Finances (annually), and State Tax Collections (annually); and state tax handbooks published annually by Research Institute of America (RIA) and Commerce Clearing House (CCH). You can find valuable data at the Bureau of Economic Analysis (www.bea.gov) and Bureau of Census (www.census.gov) websites. From those and similar sources, prepare answers to these questions about the revenue system in your state:

a. How does the burden of state, local, and state-local taxation in your state compare with that of the nation and region? (Comparisons are often made as percentages of state personal income and per capita income. What is the logical difference between the two measures?) State and local tax collections for each state are available at the website of the U.S. Bureau of Census (www. census.gov); look under the section labeled “Public Sector.” How does the local share of state and local taxes compare?

b. Prepare an estimate of the relationship between business and individual tax shares for your state. How do you decide what is a business tax and what is an individual tax?

c. How rapidly have state and local taxes grown in your state during the past five years? Is that faster or slower than growth in state personal income and the rate of inflation? Have there been tax increases (decreases) affecting that growth?

d. What are the major revenue sources used by governments in your state? How does relative use of those sources compare with the nation and the region? Does your state have any major taxes not common to other states (severance, business and occupation, local income, etc.)? Are some typical taxes not used?

My state is Arkansas. Please provide the website address for each question.

Your client asks your opinion about different weight loss options beyond diet and exercise. Her BMI is 37 and she has type 2 diabetes, high blood pressure and high cholesterol. What clinical weight loss procedures is she eligible for based on the clinical continuum or care for obesity? How did you determine this?

*Please explain in most detail possible*

Write about 300 words:

While over the long run, the economy grows about 2 to 3% per year on average, over the shorter term, the economy goes through business cycles. Think about the growth rate of GDP, the inflation rate, and the unemployment rate over the last 12 months. What conclusions about the state of the economy? Would you describe the economy as booming, recovering, or in recession during the last few years? Why? Use the AD-AS model to describe the economy. Which curve do you think caused the change? Explain your reasoning.

An urn contains 5 red balls and 5 blue balls.

​(a) If 3 balls are selected all at​ once, what is the probability that 2 are blue and 1 is​ red?

​(b) If 3 balls are selected by pulling out a​ ball, noting its​ color, and putting it back in the urn before the next​ section, what is the probability that only the first and third balls drawn are​ blue? ​

(c) If 3 balls are selected one at a time without putting them back in the​ urn, what is the probability that only the first and third balls are​ blue? ​

(a) The probability that 2 balls are blue and 1 is red is__? ​

(b) The probability is __? ​

(c) The probability is __? ​