What are the human resources topics that are currently highlighted by the Society for Human Resource Management?

What are the some interesting developments noted in the HR industry?

What are some challenges that you can assess through the topics and communities?

In product design for human use and recommended guideline for the product’s human use, it is important to consider the weights of people so that airplanes or elevators aren’t overloaded. Based on data from the National Health Survey, the weight of adults in the United States has a mean of 181 pound (the average of 195.5 for males and 166.2 for females, assuming, arbitrarily, equal male and female population) with a standard deviation of 30 pounds. An airplane is designed to have a human carrying maximum-capacity 18,500 pounds. An Airline adopts the operation procedure of maximum passenger number (n), based on the concept of the probability of a randomly selected n passengers exceeding the maximum-capacity to be less than 0.01 (i.e., 1%). a) [3 pts] Determine n b) b) [2 pts] What will be the value of n if the probability of exceeding the maximum-capacity is no more than 0.001 (i.e., 0.1%)?

Q 6-14 Multiple Choice In the space provided after the question #, record A if 1, 2, 3 are all correct B if 1 & 3 are correct C if 2 & 4 are correct D if 4 is only correct E if all are correct 6 During glucose tolerance testing: 1 The patient starts in a fasting state 2 Collection of blood is done at timed intervals 3 Used to detect hypo and hyper glycemia 4 Glucose is administered at timed intervals 7 Blood glucose: 1 Is low in hyperglycemia 2 Is elevated by insulin 3 Is not influenced by diet or exercise 4 Determinations are demonstrated most specifically by enzyme methods 8 The glucose renal threshold 1 Is the blood glucose concentration above which glucose is excreted in the urine 2 May differ slightly amongst individuals 3 Is a regulating effect that the kidney controls 4 Is most commonly exceeded in diabetes mellitus 9 Glucose determinations can be done on 1 Whole blood 2 Serum 3 Plasma 4 Cerebrospinal fluid 10 Normally blood sugar not oxidized at once for energy is: 1 Stored in the liver as glycogen 2 Stored in the muscles as glycogen 3 Converted to fat and stored in the adipose tissue of the body 4 Excreted by the kidneys 11 Hormones that have the effect of raising blood glucose concentrations is(are) 1 Growth hormone (GH) 2 Adrenocorticotropic hormone (ACTH) 3 Thyroxin 4 Insulin 12 Capillary blood specimens for glucose determination 1 Are used for point of care (POCT) home monitoring 2 Is thought of as venous rather than arterial blood 3 Is 5 mg/dL higher than venipuncture glucose 4 Must have anticoagulant added prior to testing 13 Blood obtained by venipuncture may show falsely decreased glucose concentration 1 Due to glycolysis 2 Due to enzymes present in blood cells 3 And may be avoided by storing as cell-free plasma 4 By using sodium fluoride anticoagulant 14 Sources of error when using blood glucose reflectance meters are 1 Variations in the amount of blood used 2 Washing and wiping of strips 3 Calibration of the meter 4 Using capillary blood rather than venous blood

Match the following descriptions of enzymatic activity (found on the right) to the enzyme on the left (10 points). _____ DNA polymerase III _____ RNA primase _____ DNA ligase _____ Helicase _____ RNase H degrades RNA bound to a DNA template synthesizes new phosphodiester bonds between ribonucleotides to form an oligoribonucleotide used later in DNA replication synthesizes new phosphodiester bonds between deoxyribonucleotides catalyzes condensation reactions between phosphate and hydroxyl groups Causes the H-bonding in base pairing to be interrupted so that other enzymes may bind to the deoxynucleic acid strand.

Which of the following statements characterize a restriction enzyme?

Most restriction enzymes recognize a DNA sequences between

Agarose is used in gel electrophoresis instead of polyacrylamide to separate smaller, positively charged DNA.

Which characteristic of eukaryotic mRNA allows for its separation from other nuclec acid molecules?

A cDNA library is produced by copying mRNA which has been isolated from a eukaryotic cell. Why is mRNA used to create the cDNA instead of the eukaryote's DNA itself?

the process of transforming bacteria using a cDNA and then isolating the transformed cDNA. If the growing bacteria are meant for protein synthesis, what is essential?

If the nucleotide base pictured below were to be added to a growing DNA polynucleotide chain during the dideoxy method (Sanger Method), the polynucleotide chain would stop growing at this point.

The autoradiogram shown below represents the complement sequence of a cloned DNA fragment by dideoxy sequencing. The first five nucleotides present in the 3'-end of the fragment would be?

Trypsin is an enzyme that can digest the hydrophilic portions of membrane proteins, but it is unable to penetrate the lipid bilayer and enter a cell. Due to these properties, trypsin has been used in conjunction with SDS-PAGE to determine what proteins have an extracellular domain. Describe an experiment using trypsin to determine the sidedness of proteins of the erythrocyte membrane. BE SURE TO INCLUDE ANY RELEVANT CONTROLS

A protease is an enzyme that catalyzes the hydrolysis of the peptide bonds of target proteins. How might a protease bind a target protein so that its main chain becomes fully extended in the vicinity of the vulnerable peptide bond?

The hint that we got was this: look for a way the protease might stabilize the substrate during hydrolysis

I'm open to several possibilities at this point but please explain your answer in detail.

The PFK1 enzyme is regulated by a number of factors in the cell, including the level of ATP. ATP is not only

involved in the reaction the enzyme catalyses but also binds to an allosteric site leading to a reduction in

activity.

The binding of ATP can be measured using a fluorescently labelled derivative of ATP – TNP-ATP. The data

presented below represents the determination of the binding of ATP to PFK. The change in fluorescence (?

fluor.) represents the concentration of PFK1 with TNP-ATP bound. This value was determined at 5

different concentrations of TNP-ATP, and the experiment was carried out in triplicate.

a) Create a double reciprocal plot of this data (1/[TNP-ATP] vs. 1/ ? fluor.). Plot the data from

each experiment as a separate dataset on the same graph. Only plot the data points – do

not join data points with lines. Ensure that each dataset is distinct from the others (use

alternate colours and/or shapes for the data points).

b) Fit an equation to each dataset separately. Do not show these on your plots. For each

experiment give the fitted equation and the value of Kd. Show these in a table. Ensure you

include the units of this value. Show your working for the Experiment 1 data.

c) Determine the mean and standard deviation of the data for each concentration of TNPATP.

Show these in a table. Transform this data as you have done in (a). Fit an equation to

the transformed data. Give the fitted equation and the calculated Kd for the averaged

data. Add a trendline defined by this equation to your graph.

d) With reference the data you plotted and Kd values you determined, explain the positive

and negative aspects of using a double reciprocal plot.

The enzyme is studied in the presence of a different inhibitor (inhibitor B). In this case, two different concentrations of inhibitor are used. Data are as follows:

a) Determine the apparent Vmax at each inhibitor concentration.

b) Determine the apparent KM at each inhibitor concentration.

c) Estimate KI from these data. [Hint: Calculate the K_I at 3 mM and at 5 mM; and then take the average of the two K_{I }values.]

Urea was hydrolyzed with an enzyme at a fixed concentration of 50 μM and the following data were obtained. Using Lineweaver-Burk plot determine the kinetic parameters.