The Embden-Meyerhof, the pentose phosphate, and the Entner-Doudoroff pathways are all examples of . . .  

Briefly explain how the enzyme urease is utilized by certain bacteria.

What role does diffusion play in enzyme catalyzed reactions?

How is enzyme activity (binding to substrate) detected in the ELISA test?

competitive and non-competitive inhibition of enzyme function, and why it is important.

please i want computer typing answer for A and B

The following techniques will be used for purification of an enzyme from cultured cells; salt fractionation, affinity chromatography, ion-exchange chromatography, homogenization and gel filtration chromatography. The protocol states that the techniques should be used in the order listed above.

a) What is wrong with the order of techniques as they are listed?

b) How does each technique contribute to the process of obtaining a pure enzyme?

pBR322 is a plasmid. It has two antibiotic resistance genes a the amp gene encoding the ampicillin resistance protein and the gene yet encoding the tetracycline resistance protein. In the plasmid, there is only one EcoRV restriction enzyme recognition sequence within the Tet gene. One EcoRI restriction enzyme recognition sequence between Tet gene and amp gene. Is amp gene a regulated gene or nonregulated gene. justify

2. a. explain how a mutation in pyruvate dehydrogenase kinase that increases the enzymes Km will affect the activity of the complex pyruvate dehydrogenase?

b. explain how pyruvate dehydrogenase complex is controlled during physical activity by PDP?

c. how can a mutation that affects the binding sight of calcium ions on the pdp enzyme affect the activity of phosphatase enzyme?

and how is it likely to affect pdc during physical activity?

There are hundreds of different restriction enzymes, each of which cuts a specific target DNA sequence.

a. What are the two types of cuts different restriction enzymes can make?

b. In a cloning procedure, why would you use the same restriction enzyme to excise the target DNA sequence and to cut the plasmid? (1 mark)

c. What enzyme would you use for the next step and what would it do?

You want a 50 µL reaction volume containing 1X reaction buffer, 1 µg DNA, and 10 Units of enzyme.You have a plasmid with a concentration of 250 ng/µL, 10X reaction buffer, and your enzyme concentration is 20,000 units/mL.

How many µL of water, reaction buffer, DNA and enzyme would you combine to set up that reaction? Please show work